Nanoparticle-Based Rifampicin Delivery System Development
Abstract
:1. Introduction
2. Results and Discussion
2.1. Evaluation of Size, Morphology, and ζ-Potential of PENs
2.2. Evaluation of Colloidal Stability of PENs
2.3. HNMR, Elemental Analysis and Infrared Spectrophotometry Analysis
2.4. XRD Analysis
2.5. Calorimetric Analysis
2.6. In Vitro Release Studies
2.7. Degradation Studies
3. Materials and Methods
3.1. Materials
3.2. Synthesizing and Characterization of CS Derivatives
3.3. Synthesizing and Characterization of PENs
3.4. Drug Loading and in Vitro Release Assays of PENs
3.5. RIF Degradation Assays
3.6. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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APENs | RIF/APENs | WPENs | RIF/WPENs | ||
---|---|---|---|---|---|
SEM | Size | 296.7 ± 28.2 a | 224.1 ± 50.9 b | 235.0 ± 46.5 b | 226.4 ± 50.7 b |
TEM | Size | 271.9 ± 38.8 a | 215.0 ± 30.6 b | 220.0 ± 40.4 b | 184.9 ± 34.4 b |
DLS | size | 313.8 ± 5.2 a | 241.9 ± 4.0 b | 249.1 ± 0.8 b | 224.7 ± 1.8 c |
PDI | 0.31 ± 0.01 a | 0.31 ± 0.00 a | 0.28 ± 0.01 a | 0.29 ± 0.01 a | |
ζ-potential | 29.0 ± 0.7 a | 42.0 ± 4.2 b | 26.3 ± 0.8 ac | 38.5 ± 2.8 b |
Time (d) | Z-Average Size (nm) | PDI | Z-Average Size (nm) | PDI | |
---|---|---|---|---|---|
1 | 302.1 ± 5.2 | 0.37 ± 0.03 | 233.5 ± 9.4 | 0.19 ± 0.02 | |
H2O | 7 | 321.8 ± 13.3 | 0.27 ± 0.00 | 243.9 ± 2.0 | 0.25 ± 0.00 |
14 | 304.2 ± 7.4 | 0.25 ± 0.00 | 249.5 ± 5.2 | 0.18 ± 0.01 | |
PBS 10mM | 7 | 311.4 ± 19.4 | 0.22 ± 0.01 | 240.0 ± 5.7 | 0.25 ± 0.00 |
14 | 294.1 ± 10.4 | 0.18 ± 0.01 | 239.1 ± 3.5 | 0.20 ± 0.01 |
A-PENs | W-PENs | ||
---|---|---|---|
pH 7.4 | No ASC | 72.8 ± 1.1 a | 68.3 ± 3.0 a |
ASC | 75.5 ± 2.4 a | 80.8 ± 13.0 a | |
pH 8.5 | No ASC | 31.8 ± 2.1 b | 32.1 ± 2.8 b |
ASC | 113.5 ± 6.0 c | 112.3 ± 4.7 c |
0 | 3 | 6 | 24 | 48 | 72 | |||
---|---|---|---|---|---|---|---|---|
ASC | pH 7.4 | 0.0 ± 0.0 | 1.1 ± 0.0 a | 2.4 ± 0.2 a | 8.9 ± 0.2 a | 28.8 ± 0.1 a | 58.3 ± 0.0 a | |
pH 8.5 | 0.1 ± 0.0 | 0.1 ± 0.2 b | 1.7 ± 0.1 b | 14.1 ± 0.1 b | 46.8 ± 0.1 b | 72.2 ± 0.1 b | ||
RIF | pH 7.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 5.1 ± 0.1 a | 20.7 ± 0.1 a | 37.1 ± 0.2 a | 50.6 ± 0.4 a | |
pH 8.5 | 0.0 ± 0.0 | 0.1 ± 0.0 | 7.0 ± 0.2 b | 23.7 ± 0.2 b | 50.3 ± 0.4 b | 70.4 ± 0.1 b | ||
ASC, pH 7.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 c | 0.0 ± 0.0 c | 3.1 ± 0.3 c | 9.2 ± 0.4 c | ||
ASC, pH 8.5 | 0.0 ± 0.0 | 0.1 ± 0.0 | 0.9 ± 0.1 d | 5.2 ± 0.1 d | 8.8 ± 0.1 d | 12.6 ± 0.1 d | ||
RIF/PENs | pH 7.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 4.1 ± 0.2 e | 4.1 ± 1.2 c | 3.7 ± 0.3 e | |
pH 8.5 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 5.4 ± 0.5 f | 20.4 ± 0.2 e | 42.4 ± 1.7 f | ||
ASC, pH 7.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 12.5 ± 1.1 b | 24.1 ± 2.7 e | 44.6 ± 4.6 a | ||
ASC, pH 8.5 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 1.0 ± 0.0 g | 3.2 ± 0.1 c | 21.6 ± 3.0 g |
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Motiei, M.; Pleno de Gouveia, L.; Šopík, T.; Vícha, R.; Škoda, D.; Císař, J.; Khalili, R.; Domincová Bergerová, E.; Münster, L.; Fei, H.; et al. Nanoparticle-Based Rifampicin Delivery System Development. Molecules 2021, 26, 2067. https://doi.org/10.3390/molecules26072067
Motiei M, Pleno de Gouveia L, Šopík T, Vícha R, Škoda D, Císař J, Khalili R, Domincová Bergerová E, Münster L, Fei H, et al. Nanoparticle-Based Rifampicin Delivery System Development. Molecules. 2021; 26(7):2067. https://doi.org/10.3390/molecules26072067
Chicago/Turabian StyleMotiei, Marjan, Luis Pleno de Gouveia, Tomáš Šopík, Robert Vícha, David Škoda, Jaroslav Císař, Reza Khalili, Eva Domincová Bergerová, Lukáš Münster, Haojie Fei, and et al. 2021. "Nanoparticle-Based Rifampicin Delivery System Development" Molecules 26, no. 7: 2067. https://doi.org/10.3390/molecules26072067