Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases
Abstract
:1. Introduction
2. Results and Discussions
2.1. The Solubility of Oviductus Ranae Protein
2.2. DH of Oviductus Ranae Protein
2.3. 1,1-Diphenyl-2-picrylhydrazyl (DPPH) Radical Scavenging Activity
2.4. Reducing Power
2.5. Hydroxyl Radical Scavenging Activity
2.6. Comprehensive Antioxidant Activity Analysis
2.7. Correlation Analysis
3. Materials and Methods
3.1. Chemicals and Samples
3.2. Oviductus Ranae Protein Extraction
3.3. Determination of Solubility
3.4. Enzymatic Hydrolysis
3.5. Determination of DH
3.6. Determination of Antioxidant Activity
3.6.1. DPPH Radical Scavenging Activity
3.6.2. Determination of Reducing Power
3.6.3. Hydroxyl Radical Scavenging Activity
3.6.4. Comprehensive Analysis of Antioxidant Activity
3.7. Correlation Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Protease | Time | DH | DPPH | Reducing Power | OH | CAA | |
---|---|---|---|---|---|---|---|
Pepsin | Time | 1.000 | 0.831 ** | 0.864 ** | 0.900 ** | 0.679 * | 0.889 ** |
DH | 0.831 ** | 1.000 | 0.952 ** | 0.945 ** | 0.934 ** | 0.973 ** | |
Trypsin | Time | 1.000 | 0.892 ** | 0.717 ** | 0.954 ** | 0.772 ** | 0.903 ** |
DH | 0.892 ** | 1.000 | 0.902 ** | 0.945 ** | 0.902 ** | 0.965 ** | |
Papain | Time | 1.000 | 0.916 ** | 0.795 ** | 0.829 ** | 0.563 * | 0.809 ** |
DH | 0.916 ** | 1.000 | 0.947 ** | 0.945 ** | 0.714 ** | 0.942 ** | |
Flavourzyme | Time | 1.000 | 0.873 ** | 0.875 ** | 0.918 ** | 0.876 ** | 0.917 ** |
DH | 0.873 ** | 1.000 | 0.976 ** | 0.902 ** | 0.936 ** | 0.946 ** | |
Neutral protease | Time | 1.000 | 0.864 ** | 0.745 ** | 0.867 ** | 0.572 * | 0.815 ** |
DH | 0.864 ** | 1.000 | 0.914 ** | 0.911 ** | 0.711 ** | 0.905 ** | |
Alcalase | Time | 1.000 | 0.952 ** | 0.689 ** | 0.808 ** | 0.929 ** | 0.839 ** |
DH | 0.952 ** | 1.000 | 0.854 ** | 0.919 ** | 0.988 ** | 0.949 ** |
Protease | Origin | Optimal pH | Optimal Temperature (°C) |
---|---|---|---|
Pepsin | Porcine gastric mucosa | 2.0 | 37 |
Trypsin | Porcine pancreas | 8.0 | 37 |
Papain | Papaya plant | 6.5 | 55 |
Flavourzyme | Aspergillus oryzae | 6.5 | 50 |
Neutral protease | Bacillus subtilis | 7.0 | 55 |
Alcalase | Bacillus licheniformis | 10.0 | 45 |
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Wang, S.; Gan, Y.; Mao, X.; Kan, H.; Li, N.; Zhang, C.; Wang, Z.; Wang, Y. Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases. Molecules 2021, 26, 1625. https://doi.org/10.3390/molecules26061625
Wang S, Gan Y, Mao X, Kan H, Li N, Zhang C, Wang Z, Wang Y. Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases. Molecules. 2021; 26(6):1625. https://doi.org/10.3390/molecules26061625
Chicago/Turabian StyleWang, Shihan, Yuanshuai Gan, Xinxin Mao, Hong Kan, Nan Li, Changli Zhang, Zhihan Wang, and Yongsheng Wang. 2021. "Antioxidant Activity Evaluation of Oviductus Ranae Protein Hydrolyzed by Different Proteases" Molecules 26, no. 6: 1625. https://doi.org/10.3390/molecules26061625