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Article

Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays

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ZIEL-Institute for Food & Health, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Weihenstephaner Berg 1, 85354 Freising, Germany
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Fraunhofer Institue for Process Engineering and Packaging IVV, Giggenhauser Str. 35, 85354 Freising, Germany
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Chair of Food Packaging Technology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Weihenstephaner Steig 22, 85354 Freising, Germany
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Chair of Food Science, Institute for Nutritional and Food Sciences, University of Bonn, Meckenheimer Allee 166a, 53113 Bonn, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Renata Bilewicz
Molecules 2021, 26(5), 1244; https://doi.org/10.3390/molecules26051244
Received: 23 January 2021 / Revised: 19 February 2021 / Accepted: 23 February 2021 / Published: 25 February 2021
Numerous assays were developed to measure the antioxidant activity, but each has limitations and the results obtained by different methods are not always comparable. Popular examples are the DPPH and ABTS assay. Our aim was to study similarities and differences of these two assay regarding the measured antioxidant potentials of 24 phenolic compounds using the same measurement and evaluation methods. This should allow conclusions to be drawn as to whether one of the assays is more suitable for measuring specific subgroups like phenolic acids, flavonols, flavanones, dihydrochalcones or flavanols. The assays showed common trends for the mean values of most of the subgroups. Some dihydrochalcones and flavanones did not react with the DPPH radical in contrast to the ABTS radical, leading to significant differences. Therefore, to determine the antioxidant potential of dihydrochalcone or flavanone-rich extracts, the ABTS assay should be preferred. We found that the results of the flavonoids in the DPPH assay were dependent on the Bors criteria, whereas the structure–activity relationship in the ABTS assay was not clear. For the phenolic acids, the results in the ABTS assay were only high for pyrogallol structures, while the DPPH assay was mainly determined by the number of OH groups. View Full-Text
Keywords: antioxidant capacity; flavonoids; phenolic acids; reaction kinetics; stoichiometry antioxidant capacity; flavonoids; phenolic acids; reaction kinetics; stoichiometry
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MDPI and ACS Style

Platzer, M.; Kiese, S.; Herfellner, T.; Schweiggert-Weisz, U.; Miesbauer, O.; Eisner, P. Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays. Molecules 2021, 26, 1244. https://doi.org/10.3390/molecules26051244

AMA Style

Platzer M, Kiese S, Herfellner T, Schweiggert-Weisz U, Miesbauer O, Eisner P. Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays. Molecules. 2021; 26(5):1244. https://doi.org/10.3390/molecules26051244

Chicago/Turabian Style

Platzer, Melanie, Sandra Kiese, Thomas Herfellner, Ute Schweiggert-Weisz, Oliver Miesbauer, and Peter Eisner. 2021. "Common Trends and Differences in Antioxidant Activity Analysis of Phenolic Substances Using Single Electron Transfer Based Assays" Molecules 26, no. 5: 1244. https://doi.org/10.3390/molecules26051244

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