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HR-MAS NMR Applications in Plant Metabolomics

Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands
Institute of Medical Physics and Biophysics, University of Leipzig, Härtelstr. 16–17, D-04107 Leipzig, Germany
Authors to whom correspondence should be addressed.
Academic Editor: Alan Wong
Molecules 2021, 26(4), 931;
Received: 22 December 2020 / Revised: 5 February 2021 / Accepted: 6 February 2021 / Published: 10 February 2021
(This article belongs to the Special Issue The NMR-Based Metabolomics)
Metabolomics is used to reduce the complexity of plants and to understand the underlying pathways of the plant phenotype. The metabolic profile of plants can be obtained by mass spectrometry or liquid-state NMR. The extraction of metabolites from the sample is necessary for both techniques to obtain the metabolic profile. This extraction step can be eliminated by making use of high-resolution magic angle spinning (HR-MAS) NMR. In this review, an HR-MAS NMR-based workflow is described in more detail, including used pulse sequences in metabolomics. The pre-processing steps of one-dimensional HR-MAS NMR spectra are presented, including spectral alignment, baseline correction, bucketing, normalisation and scaling procedures. We also highlight some of the models which can be used to perform multivariate analysis on the HR-MAS NMR spectra. Finally, applications of HR-MAS NMR in plant metabolomics are described and show that HR-MAS NMR is a powerful tool for plant metabolomics studies. View Full-Text
Keywords: metabolomics; plants; HR-MAS NMR; multivariate analysis metabolomics; plants; HR-MAS NMR; multivariate analysis
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MDPI and ACS Style

Augustijn, D.; de Groot, H.J.M.; Alia, A. HR-MAS NMR Applications in Plant Metabolomics. Molecules 2021, 26, 931.

AMA Style

Augustijn D, de Groot HJM, Alia A. HR-MAS NMR Applications in Plant Metabolomics. Molecules. 2021; 26(4):931.

Chicago/Turabian Style

Augustijn, Dieuwertje, Huub J.M. de Groot, and A. Alia 2021. "HR-MAS NMR Applications in Plant Metabolomics" Molecules 26, no. 4: 931.

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