Next Article in Journal
Assessing the Functional Properties of TiZr Nanotubular Structures for Biomedical Applications, through Nano-Scratch Tests and Adhesion Force Maps
Next Article in Special Issue
Photosynthetic Light-Harvesting (Antenna) Complexes—Structures and Functions
Previous Article in Journal
Synthesis, Antitumor and Antibacterial Studies of New Shortened Analogues of (KLAKLAK)2-NH2 and Their Conjugates Containing Unnatural Amino Acids
Previous Article in Special Issue
Zeaxanthin, a Molecule for Photoprotection in Many Different Environments
 
 
Article

Limitations of Linear Dichroism Spectroscopy for Elucidating Structural Issues of Light-Harvesting Aggregates in Chlorosomes

1
Spectroscopy of Soft Matter, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany
2
University of Groningen, Zernike Institute for Advanced Materials, Nijenborgh 4, 9747 AG Groningen, The Netherlands
3
Bayreuth Institute for Macromolecular Research (BIMF), University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany
4
Bavarian Polymer Institute, University of Bayreuth, Universitätsstr. 30, 95440 Bayreuth, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Leszek Fiedor
Molecules 2021, 26(4), 899; https://doi.org/10.3390/molecules26040899
Received: 22 December 2020 / Revised: 26 January 2021 / Accepted: 2 February 2021 / Published: 9 February 2021
Linear dichroism (LD) spectroscopy is a widely used technique for studying the mutual orientation of the transition-dipole moments of the electronically excited states of molecular aggregates. Often the method is applied to aggregates where detailed information about the geometrical arrangement of the monomers is lacking. However, for complex molecular assemblies where the monomers are assembled hierarchically in tiers of supramolecular structural elements, the method cannot extract well-founded information about the monomer arrangement. Here we discuss this difficulty on the example of chlorosomes, which are the light-harvesting aggregates of photosynthetic green-(non) sulfur bacteria. Chlorosomes consist of hundreds of thousands of bacteriochlorophyll molecules that self-assemble into secondary structural elements of curved lamellar or cylindrical morphology. We exploit data from polarization-resolved fluorescence-excitation spectroscopy performed on single chlorosomes for reconstructing the corresponding LD spectra. This reveals that LD spectroscopy is not suited for benchmarking structural models in particular for complex hierarchically organized molecular supramolecular assemblies. View Full-Text
Keywords: linear dichroism; molecular aggregates; light harvesting; chlorosomes; photosynthesis linear dichroism; molecular aggregates; light harvesting; chlorosomes; photosynthesis
Show Figures

Figure 1

MDPI and ACS Style

Günther, L.M.; Knoester, J.; Köhler, J. Limitations of Linear Dichroism Spectroscopy for Elucidating Structural Issues of Light-Harvesting Aggregates in Chlorosomes. Molecules 2021, 26, 899. https://doi.org/10.3390/molecules26040899

AMA Style

Günther LM, Knoester J, Köhler J. Limitations of Linear Dichroism Spectroscopy for Elucidating Structural Issues of Light-Harvesting Aggregates in Chlorosomes. Molecules. 2021; 26(4):899. https://doi.org/10.3390/molecules26040899

Chicago/Turabian Style

Günther, Lisa M., Jasper Knoester, and Jürgen Köhler. 2021. "Limitations of Linear Dichroism Spectroscopy for Elucidating Structural Issues of Light-Harvesting Aggregates in Chlorosomes" Molecules 26, no. 4: 899. https://doi.org/10.3390/molecules26040899

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop