Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS
Abstract
:1. Introduction
2. Results
2.1. Detection of Naturally Occurring Peptides in the Standard Urine Sample
2.2. Variability of Signal Intensities of Individual Peptides
2.3. Variability of Biomarker Panels
2.4. Characterization of the Urinary Peptidome Representative of a Healthy Individual
3. Discussion
4. Conclusions
5. Methods
5.1. Urine Sample
5.2. Capillary Electrophoresis Mass Spectrometry
5.3. MS Data Evaluation
5.4. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Inter-Day | Intra-Day | ||||||||
---|---|---|---|---|---|---|---|---|---|
Mass [Da] | CE Time [Min] | Sequence | Gene Symbol | Mean log2 Int | SD | CV [%] | Mean log2 Int | SD | CV [%] |
1754.92 | 31.39 | SGSVIDQSRVLNLGPIT | UMOD | 15.56 | 0.33 | 2.12 | 15.48 | 0.17 | 1.07 |
3457.61 | 31.46 | NTGAPGSpGVSGpKGDAGQpGEKGSpGAQGppGAPGPLG | COL3A1 | 15.05 | 0.42 | 2.76 | 15.29 | 0.18 | 1.18 |
2248.99 | 26.16 | GGpGSDGKPGppGSQGESGRPGPpG | COL3A1 | 14.94 | 0.20 | 1.33 | 14.83 | 0.14 | 0.98 |
1882.80 | 20.24 | DEAGSEADHEGTHSTKRG | FGA | 14.87 | 0.37 | 2.49 | 14.64 | 0.13 | 0.91 |
2825.28 | 24.45 | ERGEAGIpGVpGAKGEDGKDGSpGEpGANG | COL3A1 | 14.76 | 0.28 | 1.92 | 14.72 | 0.13 | 0.88 |
1250.56 | 28.00 | ApGDRGEpGPPGp | COL1A1 | 14.65 | 0.18 | 1.26 | 14.34 | 0.11 | 0.75 |
2169.97 | 26.10 | NSGEpGApGSKGDTGAKGEPGpVG | COL1A1 | 14.61 | 0.21 | 1.46 | 14.45 | 0.16 | 1.10 |
2047.92 | 21.93 | NGDDGEAGKpGRpGERGPPGP | COL1A1 | 14.37 | 0.62 | 4.32 | 14.33 | 0.23 | 1.62 |
1911.05 | 25.23 | SGSVIDQSRVLNLGPITR | UMOD | 14.34 | 0.82 | 5.75 | 11.29 | 0.18 | 1.61 |
3441.61 | 31.36 | DGAPGQKGEMGPAGPTGPRGFpGppGPDGLPGSMGPP | COL4A1 | 14.04 | 0.33 | 2.36 | 14.49 | 0.23 | 1.57 |
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Mavrogeorgis, E.; Mischak, H.; Latosinska, A.; Siwy, J.; Jankowski, V.; Jankowski, J. Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS. Molecules 2021, 26, 7260. https://doi.org/10.3390/molecules26237260
Mavrogeorgis E, Mischak H, Latosinska A, Siwy J, Jankowski V, Jankowski J. Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS. Molecules. 2021; 26(23):7260. https://doi.org/10.3390/molecules26237260
Chicago/Turabian StyleMavrogeorgis, Emmanouil, Harald Mischak, Agnieszka Latosinska, Justyna Siwy, Vera Jankowski, and Joachim Jankowski. 2021. "Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS" Molecules 26, no. 23: 7260. https://doi.org/10.3390/molecules26237260