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Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS

Mosaiques Diagnostics GmbH, 30659 Hannover, Germany
Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University Hospital, 52074 Aachen, Germany
Experimental Vascular Pathology, Cardiovascular Research Institute Maastricht (CARIM), University of Maastricht, 6229 Maastricht, The Netherlands
Author to whom correspondence should be addressed.
Academic Editor: Daniela Rigano
Molecules 2021, 26(23), 7260;
Received: 29 October 2021 / Revised: 19 November 2021 / Accepted: 28 November 2021 / Published: 30 November 2021
In recent years, capillary electrophoresis coupled to mass spectrometry (CE-MS) has been increasingly applied in clinical research especially in the context of chronic and age-associated diseases, such as chronic kidney disease, heart failure and cancer. Biomarkers identified using this technique are already used for diagnosis, prognosis and monitoring of these complex diseases, as well as patient stratification in clinical trials. CE-MS allows for a comprehensive assessment of small molecular weight proteins and peptides (<20 kDa) through the combination of the high resolution and reproducibility of CE and the distinct sensitivity of MS, in a high-throughput system. In this study we assessed CE-MS analytical performance with regards to its inter- and intra-day reproducibility, variability and efficiency in peptide detection, along with a characterization of the urinary peptidome content. To this end, CE-MS performance was evaluated based on 72 measurements of a standard urine sample (60 for inter- and 12 for intra-day assessment) analyzed during the second quarter of 2021. Analysis was performed per run, per peptide, as well as at the level of biomarker panels. The obtained datasets showed high correlation between the different runs, low variation of the ten highest average individual log2 signal intensities (coefficient of variation, CV < 10%) and very low variation of biomarker panels applied (CV close to 1%). The findings of the study support the analytical performance of CE-MS, underlining its value for clinical application. View Full-Text
Keywords: biomarker; capillary electrophoresis; mass spectrometry; peptides; proteomics; urine biomarker; capillary electrophoresis; mass spectrometry; peptides; proteomics; urine
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MDPI and ACS Style

Mavrogeorgis, E.; Mischak, H.; Latosinska, A.; Siwy, J.; Jankowski, V.; Jankowski, J. Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS. Molecules 2021, 26, 7260.

AMA Style

Mavrogeorgis E, Mischak H, Latosinska A, Siwy J, Jankowski V, Jankowski J. Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS. Molecules. 2021; 26(23):7260.

Chicago/Turabian Style

Mavrogeorgis, Emmanouil, Harald Mischak, Agnieszka Latosinska, Justyna Siwy, Vera Jankowski, and Joachim Jankowski. 2021. "Reproducibility Evaluation of Urinary Peptide Detection Using CE-MS" Molecules 26, no. 23: 7260.

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