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Article

LINC02532 Contributes to Radiosensitivity in Clear Cell Renal Cell Carcinoma through the miR-654-5p/YY1 Axis

1
Department of Urology, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China
2
Department of Urology, Affiliated Hospital of Sergeant School of Army Medical University, Shijiazhuang 050044, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Wenhu Zhou, Biwu Liu, Yanjing Yang and Roger Strömberg
Molecules 2021, 26(22), 7040; https://doi.org/10.3390/molecules26227040
Received: 8 September 2021 / Revised: 10 November 2021 / Accepted: 12 November 2021 / Published: 22 November 2021
(This article belongs to the Special Issue Theranostic Applications of Functional Nucleic Acids)
Background: Studies have shown that long non-coding RNAs (lncRNAs) play essential roles in tumor progression and can affect the response to radiotherapy, including in clear cell renal cell carcinoma (ccRCC). LINC02532 has been found to be upregulated in ccRCC. However, not much is known about this lncRNA. Hence, this study aimed to investigate the role of LINC02532 in ccRCC, especially in terms of radioresistance. Methods: Quantitative real-time PCR was used to detect the expression of LINC02532, miR-654-5p, and YY1 in ccRCC cells. Protein levels of YY1, cleaved PARP, and cleaved-Caspase-3 were detected by Western blotting. Cell survival fractions, viability, and apoptosis were determined by clonogenic survival assays, CCK-8 assays, and flow cytometry, respectively. The interplay among LINC02532, miR-654-5p, and YY1 was detected by chromatin immunoprecipitation and dual-luciferase reporter assays. In addition, in vivo xenograft models were established to investigate the effect of LINC02532 on ccRCC radioresistance in 10 nude mice. Results: LINC02532 was highly expressed in ccRCC cells and was upregulated in the cells after irradiation. Moreover, LINC02532 knockdown enhanced cell radiosensitivity both in vitro and in vivo. Furthermore, YY1 activated LINC02532 in ccRCC cells, and LINC02532 acted as a competing endogenous RNA that sponged miR-654-5p to regulate YY1 expression. Rescue experiments indicated that miR-654-5p overexpression or YY1 inhibition recovered ccRCC cell functions that had been previously impaired by LINC02532 overexpression. Conclusions: Our results revealed a positive feedback loop of LINC02532/miR-654-5p/YY1 in regulating the radiosensitivity of ccRCC, suggesting that LINC02532 might be a potential target for ccRCC radiotherapy. This study could serve as a foundation for further research on the role of LINC02532 in ccRCC and other cancers. View Full-Text
Keywords: clear cell renal cell carcinoma; radioresistance; LINC02532; miR-654-5p; YY1 clear cell renal cell carcinoma; radioresistance; LINC02532; miR-654-5p; YY1
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MDPI and ACS Style

Zhou, X.; Zeng, B.; Li, Y.; Wang, H.; Zhang, X. LINC02532 Contributes to Radiosensitivity in Clear Cell Renal Cell Carcinoma through the miR-654-5p/YY1 Axis. Molecules 2021, 26, 7040. https://doi.org/10.3390/molecules26227040

AMA Style

Zhou X, Zeng B, Li Y, Wang H, Zhang X. LINC02532 Contributes to Radiosensitivity in Clear Cell Renal Cell Carcinoma through the miR-654-5p/YY1 Axis. Molecules. 2021; 26(22):7040. https://doi.org/10.3390/molecules26227040

Chicago/Turabian Style

Zhou, Xiaoguang, Bowen Zeng, Yansheng Li, Haozhou Wang, and Xiaodong Zhang. 2021. "LINC02532 Contributes to Radiosensitivity in Clear Cell Renal Cell Carcinoma through the miR-654-5p/YY1 Axis" Molecules 26, no. 22: 7040. https://doi.org/10.3390/molecules26227040

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