Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes
Abstract
:1. Introduction
2. Results
2.1. Phytochemical Analysis of H. procumbens Cell Suspension Extract
2.2. In Silico Docking Simulation
2.3. H. procumbens Extract and Its Constituents VER and LEU Modulate the Gene Expression Profile of IFN-γ/IL-17A/IL-22-Stimulated Keratinocytes
2.4. Effect of H. procumbens Extract and Pure VER and LEU on Expression of Key Psoriasis-Related Proteins
2.5. Proposed Mechanism of the Anti-Psoriatic Action of H. procumbens Extract, VER and LEU in Keratinocytes
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cultivation and Extraction of H. procumbens Cell Suspension
4.3. Nuclear Magnetic Resonance (NMR)-Based Metabolite Profiling and Chromatographic Analysis
4.4. Cell Culture and Treatment
4.5. In Silico Molecular Docking
4.6. Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR)
4.7. Western Blot Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein Target | Verbascoside | Leucosceptoside A |
---|---|---|
AKT | −7.5 kcal/M; 3.2 µM | −7.2 kcal/M; 5.4 µM |
PI3K | −9.7 kcal/M; 0.08 µM | −8.8 kcal/M; 0.4 µM |
pSTAT1 | −8.5 kcal/M; 0.6 µM | −8.2 kcal/M; 1.0 µM |
JAK2 | −7.5 kcal/M; 3.2 µM | −7.2 kcal/M; 5.4 µM |
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Koycheva, I.K.; Mihaylova, L.V.; Todorova, M.N.; Balcheva-Sivenova, Z.P.; Alipieva, K.; Ferrante, C.; Orlando, G.; Georgiev, M.I. Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes. Molecules 2021, 26, 7014. https://doi.org/10.3390/molecules26227014
Koycheva IK, Mihaylova LV, Todorova MN, Balcheva-Sivenova ZP, Alipieva K, Ferrante C, Orlando G, Georgiev MI. Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes. Molecules. 2021; 26(22):7014. https://doi.org/10.3390/molecules26227014
Chicago/Turabian StyleKoycheva, Ivanka K., Liliya V. Mihaylova, Monika N. Todorova, Zhivka P. Balcheva-Sivenova, Kalina Alipieva, Claudio Ferrante, Giustino Orlando, and Milen I. Georgiev. 2021. "Leucosceptoside A from Devil’s Claw Modulates Psoriasis-like Inflammation via Suppression of the PI3K/AKT Signaling Pathway in Keratinocytes" Molecules 26, no. 22: 7014. https://doi.org/10.3390/molecules26227014