Accumulation Pattern of Flavonoids during Fruit Development of Lonicera maackii Determined by Metabolomics
Abstract
:1. Introduction
2. Results and Discussion
2.1. Overview of Metabolomics Analysis during L. maackii Fruit Development
2.2. Analysis of Differential Metabolites during Fruit Development
2.3. KEGG Annotation and Enrichment Analysis of Differential Metabolites
2.4. Accumulation Profiles of Flavonoids during Fruit Development
3. Materials and Methods
3.1. Plant Materials
3.2. Sample Extraction and UPLC–MS/MS System-Based Metabolomics Analysis
3.2.1. Sample Preparation and Extraction
3.2.2. Acquisition Conditions of Chromatography-Mass Spectrometry
3.3. Metabolomics Data Analysis
3.3.1. LC-MS Processing Data
3.3.2. Principal Component Analysis
3.3.3. Clustering Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Metabolite Name | T1 vs. T2 | T2 vs. T3 | T3 vs. T4 | T4 vs. T5 | ||||
---|---|---|---|---|---|---|---|---|
Flavone | Log2FC | VIP | Log2FC | VIP | Log2FC | VIP | Log2FC | VIP |
Isorhamnetin-3-O-rhamnoside | −12.97 | 1.14 | - | - | - | - | - | - |
Myricetin-3-O-rhamnoside | - | - | - | - | - | - | - | - |
Luteolin | - | - | - | - | −0.88 | 1.10 | - | - |
Luteolin-7-O-glucoside | −3.56 | 1.12 | 2.26 | 1.25 | - | - | −1.13 | 1.14 |
6-Hydroxyluteolin 5-glucoside | - | - | 1.89 | 1.22 | −0.95 | 1.06 | 1.42 | 1.18 |
Luteolin-di-O-glucoside | - | - | 2.13 | 1.14 | - | - | −14.79 | 1.15 |
Luteolin-O-Malonyl-O-Hexoside-O-Pentoside | - | - | 3.72 | 1.20 | - | - | - | - |
Luteolin-O-Hexoside-O-Hexoside-O-Pentoside | −1.57 | 1.09 | 1.56 | 1.22 | −0.85 | 1.06 | - | - |
Apigenin | - | - | - | - | −2.14 | 1.17 | - | - |
Apigenin 7-O-glucoside | 1.50 | 1.13 | - | - | −1.48 | 1.18 | 0.86 | 1.09 |
Apigenin 7-rutinoside | −0.80 | 1.09 | - | - | - | - | - | - |
Apigenin 6,8-C-diglucoside | - | - | - | - | - | - | −1.30 | 1.19 |
Apigenin O-hexosyl-O-rutinoside | 2.30 | 1.07 | - | - | −1.33 | 1.17 | - | - |
6-C-Hexosyl-apigenin O-feruloylhexoside | - | - | 13.58 | 1.26 | 5.90 | 1.17 | −4.69 | 1.19 |
Butin | 1.83 | 1.13 | 0.45 | 1.18 | −1.30 | 1.18 | −0.70 | 1.18 |
Chrysoeriol-7-O-rutinoside | - | - | - | - | 14.64 | 1.17 | −14.64 | 1.19 |
Chrysoeriol-di-O-glucoside | 14.65 | 1.13 | 2.41 | 1.17 | −1.53 | 1.04 | 1.48 | 1.16 |
Chrysoeriol-O-hexosyl-O-rutinoside | - | - | - | - | - | - | - | - |
Luteolin-7-O-rutinoside | −3.27 | 1.14 | 2.30 | 1.20 | 0.79 | 1.09 | −2.07 | 1.19 |
Diosmetin-7-O-Neohesperidoside | 1.95 | 1.14 | 1.13 | 1.24 | 1.06 | 1.16 | −2.60 | 1.20 |
Epicatechin | 4.34 | 1.14 | - | - | −1.83 | 1.18 | 1.77 | 1.20 |
Catechin | 4.33 | 1.14 | −0.33 | 1.10 | −2.05 | 1.17 | 1.91 | 1.19 |
Lonicerin | −3.14 | 1.14 | 2.27 | 1.23 | 0.72 | 1.14 | −2.34 | 1.20 |
Catechin gallate | - | - | 1.28 | 1.19 | - | - | - | - |
Gallocatechin-catechin | −2.70 | 1.14 | 2.26 | 1.26 | - | - | −1.30 | 1.16 |
Flavonol | Log2FC | VIP | Log2FC | VIP | Log2FC | VIP | Log2FC | VIP |
Kaempferol | - | - | - | - | 0.59 | 1.15 | −11.28 | 1.13 |
Dihydrokaempferol | 0.76 | 1.06 | 1.68 | 1.26 | - | - | −2.92 | 1.18 |
Kaempferol-3-O-arabinoside | - | - | - | - | 0.44 | 1.14 | −1.13 | 1.20 |
Kaempferol-7-O-glucosdie | −1.97 | 1.12 | 4.51 | 1.26 | 2.58 | 1.18 | −1.40 | 1.20 |
Kaempferol-3-O-glucoside | −1.66 | 1.11 | 4.24 | 1.25 | 2.63 | 1.18 | −1.34 | 1.19 |
Kaempferol-3-O-galactoside | −3.72 | 1.14 | 2.16 | 1.25 | - | - | −1.25 | 1.17 |
6-Hydroxykaempferol-7-O-glucoside | −0.36 | 1.04 | - | - | - | - | - | - |
Kaempferol-3-O-(6″-acetyl)-glucoside | 1.69 | 1.07 | - | - | - | - | 0.80 | 1.11 |
Kaempferol-3-O-(2″-trans-p-Coumaroyl)-β-d-galactopyranoside | - | - | - | - | - | - | - | - |
Kaempferol-3-O-robinobioside | −2.90 | 1.13 | 2.33 | 1.26 | - | - | −1.75 | 1.19 |
Kaempferol-3-O-rutinoside | −2.81 | 1.14 | 2.23 | 1.26 | - | - | −1.80 | 1.18 |
Kaempferol-3-O-neohesperidoside | - | - | 1.53 | 1.26 | 0.71 | 1.18 | −1.91 | 1.20 |
Kaempferol-3-O-glucoside-7-O-rhamnoside | −3.20 | 1.14 | 2.35 | 1.25 | 0.81 | 1.12 | −2.14 | 1.18 |
6-Hydroxykaempferol-3,6-O-Diglucoside | - | - | 2.82 | 1.21 | - | - | 1.19 | 1.17 |
7-O-Methxyl Quercetin | - | - | - | - | 11.96 | 1.18 | −11.96 | 1.20 |
Di-O-methylquercetin | −3.84 | 1.14 | 0.33 | 1.19 | 1.58 | 1.18 | −2.63 | 1.20 |
Quercetin-3-O-α-l-rhamnoside | - | - | - | - | - | - | - | - |
Quercetin-7-O-Glucoside | - | - | 1.01 | 1.22 | - | - | 0.16 | 1.05 |
Quercetin-3-O-glucuronide | - | - | - | - | - | - | - | - |
Quercetin-O-acetylhexoside | - | - | - | - | - | - | 1.42 | 1.15 |
Quercetin-3-O-(2″-acetyl)-β-d-glucuronide | - | - | - | - | - | - | 0.81 | 1.11 |
Quercetin-3-O-(6″-O-malonyl)-galactoside | - | - | 1.12 | 1.25 | −0.73 | 1.18 | 1.25 | 1.17 |
Quercetin-7-O-(6′-O-malonyl)-β-d-glucoside | - | - | 1.27 | 1.16 | −1.17 | 1.17 | 1.20 | 1.19 |
Quercetin-3-O-(6″-trans-p-Coumaroyl)-β-d-galactopyranoside | −13.27 | 1.14 | 15.28 | 1.26 | 4.16 | 1.18 | −3.40 | 1.20 |
Quercetin-O-feruloyl-Pentoside | −0.95 | 1.14 | 0.85 | 1.22 | 0.61 | 1.14 | −0.80 | 1.19 |
Quercetin-3-O-rutinoside (Rutin) | −0.67 | 1.01 | 0.80 | 1.09 | - | - | - | - |
Quercetin-3-O-robinobioside | −0.72 | 1.07 | 0.68 | 1.19 | - | - | - | - |
Quercetin-3-O-(2-O-α-l-rhamnopyranosyl)-β-d-galactopyranoside | −13.03 | 1.10 | 11.00 | 1.26 | - | - | - | - |
Quercetin-3-O-neohesperidoside | −1.04 | 1.06 | 0.88 | 1.13 | 0.72 | 1.10 | −0.76 | 1.15 |
Quercetin-glucoside-malonyl-glucoside | - | - | - | - | - | - | - | - |
Quercetin-O-rhamnoside-O-Hexoside-O-rhamnoside | - | - | 11.52 | 1.23 | 2.63 | 1.08 | −2.04 | 1.08 |
Quercetin-O-rutinoside-hexose | −3.62 | 1.14 | 0.96 | 1.17 | 0.70 | 1.12 | - | - |
Quercetin-glucoside-glucoside-rhamnoside | −2.70 | 1.14 | 1.14 | 1.07 | - | - | - | - |
Syringetin 3-O-hexoside | - | - | −11.92 | 1.24 | - | - | - | - |
Isorhamnetin-3-O-rutinoside | −3.25 | 1.14 | - | - | - | - | - | - |
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Qi, Z.; Zhao, R.; Xu, J.; Ge, Y.; Li, R.; Li, R. Accumulation Pattern of Flavonoids during Fruit Development of Lonicera maackii Determined by Metabolomics. Molecules 2021, 26, 6913. https://doi.org/10.3390/molecules26226913
Qi Z, Zhao R, Xu J, Ge Y, Li R, Li R. Accumulation Pattern of Flavonoids during Fruit Development of Lonicera maackii Determined by Metabolomics. Molecules. 2021; 26(22):6913. https://doi.org/10.3390/molecules26226913
Chicago/Turabian StyleQi, Zengxing, Ran Zhao, Jing Xu, Yanrui Ge, Ruofan Li, and Ruili Li. 2021. "Accumulation Pattern of Flavonoids during Fruit Development of Lonicera maackii Determined by Metabolomics" Molecules 26, no. 22: 6913. https://doi.org/10.3390/molecules26226913