Antiviral Potential of Naphthoquinones Derivatives Encapsulated within Liposomes
Abstract
:1. Introduction
2. Results
3. Discussion and Conclusions
4. Materials and Methods
4.1. Compounds
4.2. Cell Culture and Virus
4.3. Liposome Preparation
4.4. In Vitro Drug Release
4.5. Cytotoxicity Assay
4.6. Antiviral Assays
4.6.1. Yield Reduction Assay
4.6.2. Attachment Assay
4.6.3. Time-of-addition Assay
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Compound | Drug (radical) | Size (nm) | Polydispersity Index | Zeta Potential (mV) |
---|---|---|---|---|
1 | n butyl | 102.1 ± 1.1 | 0.19 ± 0.01 | −24.2 ± 0.1 |
2 | benzyl | 130.1 ± 7.2 | 0.13 ± 0.09 | −20.0 ± 0.1 |
3 | nitrobenzene | 112.6 ± 3.5 | 0.17 ± 0.02 | −13.1 ± 0.7 |
Acyclovir | 1 | 2 | 3 | |
---|---|---|---|---|
encapsulated | 13 ± 1 | 15 ± 1 | 11 ± 1 | 13 ± 2 |
free | 15 ± 1 | 19 ± 1 | 22 ± 2 | 17 ± 2 |
Drug (radical) | CC50, μM | EC50, μM(*) | SI, CC50/EC50 |
---|---|---|---|
Acyclovir | 13 ± 1 | 3.16 ± 0.09 | 4.1 |
1 (n butyl) | 15 ± 1 | 1.73 ± 0.08 | 8.7 |
2 (benzyl) | 11 ± 1 | 0.56 ± 0.02 | 20 |
3 (nitrobenzene) | 13 ± 2 | 0.36 ± 0.04 | 36 |
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Giongo, V.; Falanga, A.; De Melo, C.P.P.; da Silva, G.B.; Bellavita, R.; De-Simone, S.G.; Paixão, I.C.; Galdiero, S. Antiviral Potential of Naphthoquinones Derivatives Encapsulated within Liposomes. Molecules 2021, 26, 6440. https://doi.org/10.3390/molecules26216440
Giongo V, Falanga A, De Melo CPP, da Silva GB, Bellavita R, De-Simone SG, Paixão IC, Galdiero S. Antiviral Potential of Naphthoquinones Derivatives Encapsulated within Liposomes. Molecules. 2021; 26(21):6440. https://doi.org/10.3390/molecules26216440
Chicago/Turabian StyleGiongo, Viveca, Annarita Falanga, Camilly P. Pires De Melo, Gustavo B. da Silva, Rosa Bellavita, Salvatore G. De-Simone, Izabel C. Paixão, and Stefania Galdiero. 2021. "Antiviral Potential of Naphthoquinones Derivatives Encapsulated within Liposomes" Molecules 26, no. 21: 6440. https://doi.org/10.3390/molecules26216440