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Article

Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions

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Jerzy Haber Institute of Catalysis and Surface Chemistry Polish Academy of Science, Niezapominajek 8, 30-239 Krakow, Poland
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Faculty of Physics, Astronomy, and Applied Computer Science, Jagiellonian University, Stanisława Łojasiewicza 11, 30-348 Krakow, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Klemen Bohinc
Molecules 2021, 26(20), 6299; https://doi.org/10.3390/molecules26206299
Received: 13 September 2021 / Revised: 8 October 2021 / Accepted: 13 October 2021 / Published: 18 October 2021
The deposition kinetics of polymer particles with fibrinogen molecule coronas at bare and poly-L-lysine (PLL) modified mica was studied using the microfluid impinging-jet cell. Basic physicochemical characteristics of fibrinogen and the particles were acquired using dynamic light scattering and the electrophoretic mobility methods, whereas the zeta potential of the substrates was determined using streaming potential measurements. Subsequently, an efficient method for the preparation of the particles with coronas, characterized by a controlled fibrinogen coverage, was developed. This enabled us to carry out measurements, which confirmed that the deposition kinetics of the particles at mica vanished at pH above 5. In contrast, the particle deposition of PLL modified mica was at maximum for pH above 5. It was shown that the deposition kinetics could be adequately analyzed in terms of the mean-field approach, analogously to the ordinary colloid particle behavior. This contrasts the fibrinogen molecule behavior, which efficiently adsorbs at negatively charged substrates for the entire range pHs up to 9.7. These results have practical significance for conducting label-free immunoassays governed by the specific antigen/antibody interactions. View Full-Text
Keywords: adsorption of fibrinogen; deposition of particles with corona; fibrinogen corona at particles; microfluidic impinging-jet cell; zeta potential of protein coronas adsorption of fibrinogen; deposition of particles with corona; fibrinogen corona at particles; microfluidic impinging-jet cell; zeta potential of protein coronas
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MDPI and ACS Style

Żeliszewska, P.; Wasilewska, M.; Cieśla, M.; Adamczyk, Z. Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions. Molecules 2021, 26, 6299. https://doi.org/10.3390/molecules26206299

AMA Style

Żeliszewska P, Wasilewska M, Cieśla M, Adamczyk Z. Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions. Molecules. 2021; 26(20):6299. https://doi.org/10.3390/molecules26206299

Chicago/Turabian Style

Żeliszewska, Paulina, Monika Wasilewska, Michał Cieśla, and Zbigniew Adamczyk. 2021. "Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions" Molecules 26, no. 20: 6299. https://doi.org/10.3390/molecules26206299

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