Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract

Bourhim, Thouria; Villareal, Myra O.; Couderc, François; Hafidi, Abdellatif; Isoda, Hiroko; Gadhi, Chemseddoha

doi:10.3390/molecules26020371

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Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract

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Faculty of Sciences Semlalia, Cadi Ayyad University, Avenue Prince Moulay Abdellah, BP 2390, 40000 Marrakesh, Morocco

Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tennodai 1-1-1, Tsukuba City 305-8572, Japan

Faculty of Life and Environmental Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba City 305-8572, Japan

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UMR CNRS 5623 (National Center for Scientific Research), Paul Sabatier University, 118 Route de Narbonne, F-31062 Toulouse, France

Authors to whom correspondence should be addressed.

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These authors contributed equally to this work.

Academic Editor: Natalizia Miceli

Molecules 2021, 26(2), 371; https://doi.org/10.3390/molecules26020371

Received: 2 December 2020 / Revised: 6 January 2021 / Accepted: 9 January 2021 / Published: 12 January 2021

(This article belongs to the Special Issue Medicinal and Aromatic Plants as Source of Bioactive Compounds: Chemical Characterization and Bioactivity)

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Abstract

The use of natural products for the regulation of skin pigmentation is gaining popularity. In the present study, we evaluated the effect of argan leaves extract (ALE) on melanogenesis in B16 melanoma cells, determined its antioxidant activity, then quantified and identified its phenolic components. B16 cells were treated with various concentrations of ALE, then the cell viability and proliferation were assessed using MTT assay while the melanin content was determined using spectrophotometric methods. The expression level of tyrosinase (TYR), tyrosinase related protein-1 (TRP-1) and dopachrome tautomerase (DCT) was evaluated by Western blotting. The antioxidant activity of ALE was investigated using four different assays while UPLC-ESI-HRMS analysis was used to characterize the ALE phenolic profile. Fourteen phenolic compounds were identified, of which six are reported for the first time to be present in ALE. ALE treatment increases the melanin content of B16 cells in a dose-dependent manner without cytotoxicity. This was revealed by the observed ALE-increased expression level of TYR, DCT, and TRP-1. These bioactivities may be mainly attributed to its high flavonoids content. Argan leaves have the potential for use as a treatment for hypopigmentation disorders and as a bioactive component of cosmetic products that aim to increase pigmentation. View Full-Text

Keywords: argan leaves; melanogenesis stimulation; antioxidant activity; phenolic compounds; UPLC-ESI-HRMS

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MDPI and ACS Style

Bourhim, T.; Villareal, M.O.; Couderc, F.; Hafidi, A.; Isoda, H.; Gadhi, C. Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract. Molecules 2021, 26, 371. https://doi.org/10.3390/molecules26020371

AMA Style

Bourhim T, Villareal MO, Couderc F, Hafidi A, Isoda H, Gadhi C. Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract. Molecules. 2021; 26(2):371. https://doi.org/10.3390/molecules26020371

Chicago/Turabian Style

Bourhim, Thouria, Myra O. Villareal, François Couderc, Abdellatif Hafidi, Hiroko Isoda, and Chemseddoha Gadhi. 2021. "Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract" Molecules 26, no. 2: 371. https://doi.org/10.3390/molecules26020371

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Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract

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