Anticancer Effects of the Corchorus olitorius Aqueous Extract and Its Bioactive Compounds on Human Cancer Cell Lines
Abstract
:1. Introduction
2. Results
2.1. Identification and Quantification of Chlorogenic Acid and Isoquercetin
2.2. CO, CGA, and IQ Inhibited Human Cancer Cells’ Proliferation
2.3. CO, CGA, and IQ Inhibit Angiogenesis and Tumor Growth in Ovo
3. Discussion
4. Materials and Methods
4.1. Plant Collection and Authentication
4.2. Plant Preparation
4.3. Plant Extraction
4.4. Solid-Phase Extraction
4.5. LC-MS/MS Analysis
4.6. Cell Culture
4.7. 3-(4, 5-Dimethyl thiazolyl-2)-2, 5-Diphenyltetrazolium Bromide (MTT) Assay
4.8. Tumor Growth and Angiogenesis in the Chorioallantoic Membrane Model
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Treatment | IC50 Values | ||
---|---|---|---|
SUIT-2 | A-375 | AGS | |
CO (mg/mL) | 6.47 ± 0.11 ## | 4.05 ± 0.09 ** | 2.54 ± 0.09 *** |
CGA (µM) | 373.53 ± 11.29 ns | 306.64 ± 20.83 ns | 330.74 ± 13.19 ns |
IQ (µM) | 144.45 ± 12.34 # | 369.29 ± 23.78 * | 355.97 ± 19.93 |
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Tosoc, J.P.S.; Nuñeza, O.M.; Sudha, T.; Darwish, N.H.E.; Mousa, S.A. Anticancer Effects of the Corchorus olitorius Aqueous Extract and Its Bioactive Compounds on Human Cancer Cell Lines. Molecules 2021, 26, 6033. https://doi.org/10.3390/molecules26196033
Tosoc JPS, Nuñeza OM, Sudha T, Darwish NHE, Mousa SA. Anticancer Effects of the Corchorus olitorius Aqueous Extract and Its Bioactive Compounds on Human Cancer Cell Lines. Molecules. 2021; 26(19):6033. https://doi.org/10.3390/molecules26196033
Chicago/Turabian StyleTosoc, John Paul Sese, Olga Macas Nuñeza, Thangirala Sudha, Noureldien H. E. Darwish, and Shaker A. Mousa. 2021. "Anticancer Effects of the Corchorus olitorius Aqueous Extract and Its Bioactive Compounds on Human Cancer Cell Lines" Molecules 26, no. 19: 6033. https://doi.org/10.3390/molecules26196033