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Article

Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis

1
Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong 17546, Korea
2
Department of Food Science and Biotechnology, Gachon University, Seongnam 461-701, Korea
*
Authors to whom correspondence should be addressed.
Academic Editors: Miguel Ángel Morcillo Alonso, Jorge Rubio-Retama and Piersandro Pallavicini
Molecules 2021, 26(19), 5996; https://doi.org/10.3390/molecules26195996
Received: 21 August 2021 / Revised: 27 September 2021 / Accepted: 27 September 2021 / Published: 2 October 2021
(This article belongs to the Special Issue Inorganic Nanoparticles in Biomedical Applications)
The present study highlights the biosynthesis of silver nanoparticles (AgNPs) using culture supernatant of Massilia sp. MAHUQ-52 as well as the antimicrobial application of synthesized AgNPs against multi-drug resistant pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Well-defined AgNPs formation occurred from the reaction mixture of cell-free supernatant and silver nitrate (AgNO3) solution within 48 h of incubation. UV-visible spectroscopy analysis showed a strong peak at 435 nm, which corresponds to the surface plasmon resonance of AgNPs. The synthesized AgNPs were characterized by FE-TEM, EDX, XRD, DLS and FT-IR. From FE-TEM analysis, it was found that most of the particles were spherical shape, and the size of synthesized nanoparticles (NPs) was 15–55 nm. EDX spectrum revealed a strong silver signal at 3 keV. XRD analysis determined the crystalline, pure, face-centered cubic AgNPs. FT-IR analysis identified various functional molecules that may be involved with the synthesis and stabilization of AgNPs. The antimicrobial activity of Massilia sp. MAHUQ-52 mediated synthesized AgNPs was determined using the disk diffusion method against K. pneumoniae and S. Enteritidis. Biosynthesized AgNPs showed strong antimicrobial activity against both K. pneumoniae and S. Enteritidis. The MICs of synthesized AgNPs against K. pneumoniae and S. Enteritidis were 12.5 and 25.0 μg/mL, respectively. The MBC of biosynthesized AgNPs against both pathogens was 50.0 μg/mL. From FE-SEM analysis, it was found that the AgNPs-treated cells showed morphological changes with irregular and damaged cell walls that culminated in cell death. View Full-Text
Keywords: Massilia sp. MAHUQ-52; biosynthesis; AgNPs; K. pneumoniae; S. Enteritidis Massilia sp. MAHUQ-52; biosynthesis; AgNPs; K. pneumoniae; S. Enteritidis
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MDPI and ACS Style

Huq, M.A.; Akter, S. Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Molecules 2021, 26, 5996. https://doi.org/10.3390/molecules26195996

AMA Style

Huq MA, Akter S. Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis. Molecules. 2021; 26(19):5996. https://doi.org/10.3390/molecules26195996

Chicago/Turabian Style

Huq, Md. Amdadul, and Shahina Akter. 2021. "Biosynthesis, Characterization and Antibacterial Application of Novel Silver Nanoparticles against Drug Resistant Pathogenic Klebsiella pneumoniae and Salmonella Enteritidis" Molecules 26, no. 19: 5996. https://doi.org/10.3390/molecules26195996

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