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Article

High Throughput Screening of the NatureBank ‘Marine Collection’ in a Haemonchus Bioassay Identifies Anthelmintic Activity in Extracts from a Range of Sponges from Australian Waters

1
Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC 3010, Australia
2
Griffith Institute for Drug Discovery, School of Environment and Science, Griffith University, Brisbane, QLD 4111, Australia
3
Queensland Museum, South Brisbane, QLD 4101, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Charng-Cherng Chyau
Molecules 2021, 26(19), 5846; https://doi.org/10.3390/molecules26195846
Received: 4 August 2021 / Revised: 21 September 2021 / Accepted: 23 September 2021 / Published: 27 September 2021
(This article belongs to the Special Issue Bioactive Compounds from Nature: New Research and Prospects)
Widespread resistance in parasitic nematodes to most classes of anthelmintic drugs demands the discovery and development of novel compounds with distinct mechanisms of action to complement strategic or integrated parasite control programs. Products from nature—which assume a diverse ‘chemical space’—have significant potential as a source of anthelmintic compounds. In the present study, we screened a collection of extracts (n = 7616) derived from marine invertebrates sampled from Australian waters in a high throughput bioassay for in vitro anti-parasitic activity against the barber’s pole worm (Haemonchus contortus)—an economically important parasitic nematode of livestock animals. In this high throughput screen (HTS), we identified 58 active extracts that reduced larval motility by ≥70% (at 90 h), equating to an overall ‘hit rate’ of ~0.8%. Of these 58 extracts, 16 also inhibited larval development by ≥80% (at 168 h) and/or induced ‘non-wild-type’ (abnormal) larval phenotypes with reference to ‘wild-type’ (normal) larvae not exposed to extract (negative controls). Most active extracts (54 of 58) originated from sponges, three from chordates (tunicates) and one from a coral; these extracts represented 37 distinct species/taxa of 23 families. An analysis of samples by 1H NMR fingerprinting was utilised to dereplicate hits and to prioritise a set of 29 sponge samples for future chemical investigation. Overall, these results indicate that a range of sponge species from Australian waters represents a rich source of natural compounds with nematocidal or nematostatic properties. Our plan now is to focus on in-depth chemical investigations of the sample set prioritised herein. View Full-Text
Keywords: high throughput screening; Haemonchus contortus; parasitic nematode; anthelmintic; marine natural products; sponge high throughput screening; Haemonchus contortus; parasitic nematode; anthelmintic; marine natural products; sponge
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MDPI and ACS Style

Taki, A.C.; Byrne, J.J.; Jabbar, A.; Lum, K.Y.; Hayes, S.; Addison, R.S.; Ramage, K.S.; Hofmann, A.; Ekins, M.G.; Wang, T.; Chang, B.C.H.; Davis, R.A.; Gasser, R.B. High Throughput Screening of the NatureBank ‘Marine Collection’ in a Haemonchus Bioassay Identifies Anthelmintic Activity in Extracts from a Range of Sponges from Australian Waters. Molecules 2021, 26, 5846. https://doi.org/10.3390/molecules26195846

AMA Style

Taki AC, Byrne JJ, Jabbar A, Lum KY, Hayes S, Addison RS, Ramage KS, Hofmann A, Ekins MG, Wang T, Chang BCH, Davis RA, Gasser RB. High Throughput Screening of the NatureBank ‘Marine Collection’ in a Haemonchus Bioassay Identifies Anthelmintic Activity in Extracts from a Range of Sponges from Australian Waters. Molecules. 2021; 26(19):5846. https://doi.org/10.3390/molecules26195846

Chicago/Turabian Style

Taki, Aya C., Joseph J. Byrne, Abdul Jabbar, Kah Y. Lum, Sasha Hayes, Russell S. Addison, Kelsey S. Ramage, Andreas Hofmann, Merrick G. Ekins, Tao Wang, Bill C.H. Chang, Rohan A. Davis, and Robin B. Gasser 2021. "High Throughput Screening of the NatureBank ‘Marine Collection’ in a Haemonchus Bioassay Identifies Anthelmintic Activity in Extracts from a Range of Sponges from Australian Waters" Molecules 26, no. 19: 5846. https://doi.org/10.3390/molecules26195846

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