Inhibitory Effect of Fisetin on α-Glucosidase Activity: Kinetic and Molecular Docking Studies
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Reagents
2.2. α-Glucosidase Inhibition Assay
2.3. α-Glucosidase Kinetic Assay
2.4. Fluorescence Spectrophotometric Measurement
2.5. Homology Modeling and Molecular Docking
3. Results and Discussion
3.1. Inhibitory Effect of Fisetin on α-Glucosidase
3.2. Enzymatic Kinetic Assay
3.3. Fluorescence Quenching Studies of α-Glucosidase by Fisetin
3.4. Homology Modeling and Molecular Docking Analysis of α-Glucosidase
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
Abbreviations
References
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Shen, B.; Shangguan, X.; Yin, Z.; Wu, S.; Zhang, Q.; Peng, W.; Li, J.; Zhang, L.; Chen, J. Inhibitory Effect of Fisetin on α-Glucosidase Activity: Kinetic and Molecular Docking Studies. Molecules 2021, 26, 5306. https://doi.org/10.3390/molecules26175306
Shen B, Shangguan X, Yin Z, Wu S, Zhang Q, Peng W, Li J, Zhang L, Chen J. Inhibitory Effect of Fisetin on α-Glucosidase Activity: Kinetic and Molecular Docking Studies. Molecules. 2021; 26(17):5306. https://doi.org/10.3390/molecules26175306
Chicago/Turabian StyleShen, Beiyun, Xinchen Shangguan, Zhongping Yin, Shaofu Wu, Qingfeng Zhang, Wenwen Peng, Jingen Li, Lu Zhang, and Jiguang Chen. 2021. "Inhibitory Effect of Fisetin on α-Glucosidase Activity: Kinetic and Molecular Docking Studies" Molecules 26, no. 17: 5306. https://doi.org/10.3390/molecules26175306