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Article

Lepidium graminifolium L.: Glucosinolate Profile and Antiproliferative Potential of Volatile Isolates

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Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia
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Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia
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School of Medicine, University of Split, Šoltanska 2, 21000 Split, Croatia
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Department of Biology, Faculty of Science, University of Split, Ruđera Boškovića 33, 21000 Split, Croatia
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Biomolecular Sciences Programme, School of Biological, Chemical and Forensic Sciences, Laurentian University, 935 Ramsey Lake Road, Sudbury, ON P3E 2C6, Canada
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Institut de Chimie Organique et Analytique, Université d’Orléans et CNRS, UMR 7311, 45000 Orléans, France
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Author to whom correspondence should be addressed.
Academic Editor: Filomena Conforti
Molecules 2021, 26(17), 5183; https://doi.org/10.3390/molecules26175183
Received: 27 July 2021 / Revised: 19 August 2021 / Accepted: 23 August 2021 / Published: 27 August 2021
(This article belongs to the Special Issue Organosulfur Compounds: Their Distribution and Biological Potential)
Glucosinolates (GSLs) from Lepidium graminifolium L. were analyzed qualitatively and quantitatively by their desulfo-counterparts using UHPLC-DAD-MS/MS technique and by their volatile breakdown products-isothiocyanates (ITCs) using GC-MS analysis. Thirteen GSLs were identified with arylaliphatic as the major ones in the following order: 3-hydroxybenzyl GSL (glucolepigramin, 7), benzyl GSL (glucotropaeolin, 9), 3,4,5-trimethoxybenzyl GSL (11), 3-methoxybenzyl GSL (glucolimnanthin, 12), 4-hydroxy-3,5-dimethoxybenzyl GSL (3,5-dimethoxysinalbin, 8), 4-hydroxybenzyl GSL (glucosinalbin, 6), 3,4-dimethoxybenzyl GSL (10) and 2-phenylethyl GSL (gluconasturtiin, 13). GSL breakdown products obtained by hydrodistillation (HD) and CH2Cl2 extraction after hydrolysis by myrosinase for 24 h (EXT) as well as benzyl ITC were tested for their cytotoxic activity using MTT assay. Generally, EXT showed noticeable antiproliferative activity against human bladder cancer cell line UM-UC-3 and human glioblastoma cell line LN229, and can be considered as moderately active, while IC50 of benzyl ITC was 12.3 μg/mL, which can be considered as highly active. View Full-Text
Keywords: Lepidium graminifolium L.; glucosinolates; desulfoglucosinolates; 3,4,5-trimethoxybenzyl glucosinolate; benzyl isothiocyanate; antiproliferative activity Lepidium graminifolium L.; glucosinolates; desulfoglucosinolates; 3,4,5-trimethoxybenzyl glucosinolate; benzyl isothiocyanate; antiproliferative activity
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MDPI and ACS Style

Đulović, A.; Burčul, F.; Čulić, V.Č.; Ruščić, M.; Brzović, P.; Montaut, S.; Rollin, P.; Blažević, I. Lepidium graminifolium L.: Glucosinolate Profile and Antiproliferative Potential of Volatile Isolates. Molecules 2021, 26, 5183. https://doi.org/10.3390/molecules26175183

AMA Style

Đulović A, Burčul F, Čulić VČ, Ruščić M, Brzović P, Montaut S, Rollin P, Blažević I. Lepidium graminifolium L.: Glucosinolate Profile and Antiproliferative Potential of Volatile Isolates. Molecules. 2021; 26(17):5183. https://doi.org/10.3390/molecules26175183

Chicago/Turabian Style

Đulović, Azra, Franko Burčul, Vedrana Č. Čulić, Mirko Ruščić, Petra Brzović, Sabine Montaut, Patrick Rollin, and Ivica Blažević. 2021. "Lepidium graminifolium L.: Glucosinolate Profile and Antiproliferative Potential of Volatile Isolates" Molecules 26, no. 17: 5183. https://doi.org/10.3390/molecules26175183

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