Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts
Abstract
:1. Introduction
2. Results
2.1. Obtaining and Analyses of the Extracts
2.2. Antioxidant Activity
2.3. UV−VIS Spectroscopy Analysis
2.4. FT-IR Spectroscopy
2.5. Characterization of AgNPs by SEM-EDX Analyses
2.6. Particle Size Distribution Analyses
2.7. Antibacterial Activity
3. Discussion
4. Materials and Methods
4.1. Sample Collection and Preparation
4.2. Determination of the Chemical Composition of the Extracts of Cannabis Sativa by UHPLC-DAD-MS
4.3. Total Phenolic Content
4.4. Antioxidant Activity Assay
4.4.1. 2,2-Diphenyl-1-Picrylhydrazyl (DPPH•) Assay
4.4.2. 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Diammonium Salt (ABTS•+) Assay
4.5. Biosynthesis of AgNPs
4.6. UV−VIS Spectroscopy Analysis
4.7. FT-IR Spectroscopy
4.8. Characterization of AgNPs by SEM-EDX Analyses
4.9. Particle Size Distribution Analyses
4.10. Antibacterial Activity
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
- Musio, S.; Mussig, J.; Amaducci, S. Optimizing Hemp Fiber Production for High Performance Composite Applications. Front. Plant Sci. 2018, 9, 1702. [Google Scholar] [CrossRef] [PubMed]
- Bonini, S.A.; Premoli, M.; Tambaro, S.; Kumar, A.; Maccarinelli, G.; Memo, M.; Mastinu, A. Cannabis sativa: A comprehensive ethnopharmacological review of a medicinal plant with a long history. J. Ethnopharmacol. 2018, 227, 300–315. [Google Scholar] [CrossRef]
- Leonard, W.; Zhang, P.Z.; Ying, D.Y.; Fang, Z.X. Hempseed in food industry: Nutritional value, health benefits, and industrial applications. Compr. Rev. Food Sci. Food Saf. 2020, 19, 282–308. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Nissen, L.; Zatta, A.; Stefanini, I.; Grandi, S.; Sgorbati, B.; Biavati, B.; Monti, A. Characterization and antimicrobial activity of essential oils of industrial hemp varieties (Cannabis sativa L.). Fitoterapia 2010, 81, 413–419. [Google Scholar] [CrossRef] [PubMed]
- Ingrao, C.; Lo Giudice, A.; Bacenetti, J.; Tricase, C.; Dotelli, G.; Fiala, M.; Siracusa, V.; Mbohwa, C. Energy and environmental assessment of industrial hemp for building applications: A review. Renew. Sustain. Energy Rev. 2015, 51, 29–42. [Google Scholar] [CrossRef]
- Cassano, T.; Villani, R.; Pace, L.; Carbone, A.; Bukke, V.N.; Orkisz, S.; Avolio, C.; Serviddio, G. From Cannabis sativa to Cannabidiol: Promising Therapeutic Candidate for the Treatment of Neurodegenerative Diseases. Front. Pharmacol. 2020, 11, 124. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Andre, C.M.; Hausman, J.F.; Guerriero, G. Cannabis sativa: The Plant of the Thousand and One Molecules. Front. Plant Sci. 2016, 7, 19. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Russo, E.B.; Marcu, J. Cannabis Pharmacology: The Usual Suspects and a Few Promising Leads. In Cannabinoid Pharmacology; Kendall, D., Alexander, S.P.H., Eds.; Elsevier Inc.: Amsterdam, Nederlands, 2017; Volume 80, pp. 67–134. [Google Scholar]
- Uddin, M.S.; Al Mamun, A.; Sumsuzzman, D.M.; Ashraf, G.M.; Perveen, A.; Bungau, S.G.; Mousa, S.A.; El-Seedi, H.R.; Bin-Jumah, M.N.; Abdel-Daim, M.M. Emerging Promise of Cannabinoids for the Management of Pain and Associated Neuropathological Alterations in Alzheimer’s Disease. Front. Pharmacol. 2020, 11, 1097. [Google Scholar] [CrossRef]
- Behl, T.; Kaur, G.; Bungau, S.; Jhanji, R.; Kumar, A.; Mehta, V.; Zengin, G.; Brata, R.; ul Hassan, S.S.; Fratila, O. Distinctive Evidence Involved in the Role of Endocannabinoid Signalling in Parkinson’s Disease: A Perspective on Associated Therapeutic Interventions. Int. J. Mol. Sci. 2020, 21, 6235. [Google Scholar] [CrossRef]
- Kaur, I.; Behl, T.; Bungau, S.; Zengin, G.; Kumar, A.; El-Esawi, M.A.; Khullar, G.; Venkatachalam, T.; Arora, S. The endocannabinoid signaling pathway as an emerging target in pharmacotherapy, earmarking mitigation of destructive events in rheumatoid arthritis. Life Sci. 2020, 257, 118109. [Google Scholar] [CrossRef]
- EMCDDA. Medical Use of Cannabis and Cannabinoids: Questions and Answers for Policymaking. Available online: https://www.emcdda.europa.eu/ (accessed on 12 May 2020).
- Mittal, A.K.; Bhaumik, J.; Kumar, S.; Banerjee, U.C. Biosynthesis of silver nanoparticles: Elucidation of prospective mechanism and therapeutic potential. J. Colloid Interface Sci. 2014, 415, 39–47. [Google Scholar] [CrossRef] [PubMed]
- Anselmo, A.C.; Mitragotri, S. Nanoparticles in the clinic: An update. Bioeng. Transl. Med. 2019, 4, e10143. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kharisov, B.I.; Dias, H.V.R.; Kharissova, O.V. Mini-review: Ferrite nanoparticles in the catalysis. Arab. J. Chem. 2019, 12, 1234–1246. [Google Scholar] [CrossRef] [Green Version]
- Sportelli, M.C.; Izzi, M.; Volpe, A.; Clemente, M.; Picca, R.A.; Ancona, A.; Lugara, P.M.; Palazzo, G.; Cioffi, N. The Pros and Cons of the Use of Laser Ablation Synthesis for the Production of Silver Nano-Antimicrobials. Antibiotics 2018, 7, 67. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Abdalla, S.S.I.; Katas, H.; Azmi, F.; Busra, M.F.M. Antibacterial and Anti-Biofilm Biosynthesised Silver and Gold Nanoparticles for Medical Applications: Mechanism of Action, Toxicity and Current Status. Curr. Drug Deliv. 2020, 17, 88–100. [Google Scholar] [CrossRef]
- Bordoloi, M.; Sahoo, R.K.; Tamuli, K.J.; Saikia, S.; Dutta, P.P. Plant Extracts Promoted Preparation of Silver and Gold Nanoparticles: A Systematic Review. Nano 2020, 15, 2030001. [Google Scholar] [CrossRef] [Green Version]
- Ishak, N.A.I.; Kamarudin, S.K.; Timmiati, S.N. Green synthesis of metal and metal oxide nanoparticles via plant extracts: An overview. Mater. Res. Express 2019, 6, 112004. [Google Scholar] [CrossRef]
- Jamkhande, P.G.; Ghule, N.W.; Bamer, A.H.; Kalaskar, M.G. Metal nanoparticles synthesis: An overview on methods of preparation, advantages and disadvantages, and applications. J. Drug Deliv. Sci. Technol. 2019, 53, 101174. [Google Scholar] [CrossRef]
- Jin, S.E.; Jin, H.E. Synthesis, Characterization, and Three-Dimensional Structure Generation of Zinc Oxide-Based Nanomedicine for Biomedical Applications. Pharmaceutics 2019, 11, 575. [Google Scholar] [CrossRef] [Green Version]
- Kalantari, K.; Mostafavi, E.; Afifi, A.M.; Izadiyan, Z.; Jahangirian, H.; Rafiee-Moghaddam, R.; Webster, T.J. Wound dressings functionalized with silver nanoparticles: Promises and pitfalls. Nanoscale 2020, 12, 2268–2291. [Google Scholar] [CrossRef]
- Sanchez-Lopez, E.; Gomes, D.; Esteruelas, G.; Bonilla, L.; Lopez-Machado, A.L.; Galindo, R.; Cano, A.; Espina, M.; Ettcheto, M.; Camins, A.; et al. Metal-Based Nanoparticles as Antimicrobial Agents: An Overview. Nanomaterials 2020, 10, 292. [Google Scholar] [CrossRef] [Green Version]
- Jyoti, K.; Baunthiyal, M.; Singh, A. Characterization of silver nanoparticles synthesized using Urtica dioica Linn. leaves and their synergistic effects with antibiotics. J. Radiat. Res. Appl. Sci. 2016, 9, 217–227. [Google Scholar] [CrossRef] [Green Version]
- Kumar, S.; Singh, M.; Halder, D.; Mitra, A. Mechanistic study of antibacterial activity of biologically synthesized silver nanocolloids. Colloids Surf. A Physicochem. Eng. Asp. 2014, 449, 82–86. [Google Scholar] [CrossRef]
- Swain, S.; Barik, S.K.; Behera, T.; Nayak, S.K.; Sahoo, S.K.; Mishra, S.S.; Swain, P. Green Synthesis of Gold Nanoparticles Using Root and Leaf Extracts of Vetiveria zizanioides and Cannabis sativa and its Antifungal Activities. Bionanoscience 2016, 6, 205–213. [Google Scholar] [CrossRef]
- Amini, S.M. Preparation of antimicrobial metallic nanoparticles with bioactive compounds. Mater. Sci. Eng. C Mater. Biol. Appl. 2019, 103, 109809. [Google Scholar] [CrossRef] [PubMed]
- Hajialyani, M.; Tewari, D.; Sobarzo-Sanchez, E.; Nabavi, S.M.; Farzaei, M.H.; Abdollahi, M. Natural product-based nanomedicines for wound healing purposes: Therapeutic targets and drug delivery systems. Int. J. Nanomed. 2018, 13, 5023–5043. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Patil, M.P.; Kim, G.D. Eco-friendly approach for nanoparticles synthesis and mechanism behind antibacterial activity of silver and anticancer activity of gold nanoparticles. Appl. Microbiol. Biotechnol. 2017, 101, 79–92. [Google Scholar] [CrossRef] [PubMed]
- Roy, A.; Bulut, O.; Some, S.; Mandal, A.K.; Yilmaz, M.D. Green synthesis of silver nanoparticles: Biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Adv. 2019, 9, 2673–2702. [Google Scholar] [CrossRef] [Green Version]
- Singh, P.; Kim, Y.J.; Zhang, D.B.; Yang, D.C. Biological Synthesis of Nanoparticles from Plants and Microorganisms. Trends Biotechnol. 2016, 34, 588–599. [Google Scholar] [CrossRef] [PubMed]
- Mittal, A.K.; Chisti, Y.; Banerjee, U.C. Synthesis of metallic nanoparticles using plant extracts. Biotechnol. Adv. 2013, 31, 346–356. [Google Scholar] [CrossRef]
- Keerthana, S.; Kumar, A. Potential risks and benefits of zinc oxide nanoparticles: A systematic review. Crit. Rev. Toxicol. 2020, 50, 47–71. [Google Scholar] [CrossRef] [PubMed]
- Zhu, Y.; Wu, J.H.; Chen, M.; Liu, X.L.; Xiong, Y.J.; Wang, Y.Y.; Feng, T.; Kang, S.; Wang, X.F. Recent advances in the biotoxicity of metal oxide nanoparticles: Impacts on plants, animals and microorganisms. Chemosphere 2019, 237, 124403. [Google Scholar] [CrossRef]
- Singh, T.; Jyoti, K.; Patnaik, A.; Singh, A.; Chauhan, S.C. Spectroscopic, microscopic characterization of Cannabis sativa leaf extract mediated silver nanoparticles and their synergistic effect with antibiotics against human pathogen. Alex. Eng. J. 2018, 57, 3043–3051. [Google Scholar] [CrossRef]
- Chouhan, S.; Guleria, S. Green synthesis of AgNPs using Cannabis sativa leaf extract: Characterization, antibacterial, anti-yeast and α-amylase inhibitory activity. Mater. Sci. Energy Technol. 2020, 3, 536–544. [Google Scholar] [CrossRef]
- Mandal, S.; Marpu, S.B.; Hughes, R.; Omary, M.A.; Shi, S.Q. Green synthesis of silver nanoparticles using Cannabis sativa extracts and their anti-bacterial activity. Green Sustain. Chem. 2021, 11, 38–48. [Google Scholar] [CrossRef]
- Singh, P.; Pandit, S.; Garnaes, J.; Tunjic, S.; Mokkapati, V.; Sultan, A.; Thygesen, A.; Mackevica, A.; Mateiu, R.V.; Daugaard, A.E.; et al. Green synthesis of gold and silver nanoparticles from Cannabis sativa (industrial hemp) and their capacity for biofilm inhibition. Int. J. Nanomed. 2018, 13, 3571–3591. [Google Scholar] [CrossRef]
- Abbasi, B.H.; Zaka, M.; Hashmi, S.S.; Khan, Z. Biogenic synthesis of Au, Ag and Au-Ag alloy nanoparticles using Cannabis sativa leaf extract. IET Nanobiotechnol. 2018, 12, 277–284. [Google Scholar] [CrossRef]
- Salayová, A.; Bedlovičová, Z.; Daneu, N.; Baláž, M.; Lukáčová Bujňáková, Z.; Balážová, Ľ.; Tkáčiková, Ľ. Green synthesis of silver nanoparticles with antibacterial activity using various medicinal plant extracts: Morphology and antibacterial efficacy. Nanomaterials 2021, 11, 1005. [Google Scholar] [CrossRef]
- Shameli, K.; Bin Ahmad, M.; Al-Mulla, E.A.J.; Ibrahim, N.A.; Shabanzadeh, P.; Rustaiyan, A.; Abdollahi, Y.; Bagheri, S.; Abdolmohammadi, S.; Usman, M.S.; et al. Green Biosynthesis of Silver Nanoparticles Using Callicarpa maingayi Stem Bark Extraction. Molecules 2012, 17, 8506–8517. [Google Scholar] [CrossRef]
- Senthil, B.; Devasena, T.; Prakash, B.; Rajasekar, A. Non-cytotoxic effect of green synthesized silver nanoparticles and its antibacterial activity. J. Photochem. Photobiol. B Biol. 2017, 177, 1–7. [Google Scholar] [CrossRef]
- Vigneshwaran, N.; Ashtaputre, N.M.; Varadarajan, P.V.; Nachane, R.P.; Paralikar, K.M.; Balasubramanya, R.H. Biological synthesis of silver nanoparticles using the fungus Aspergillus flavus. Mater. Lett. 2007, 61, 1413–1418. [Google Scholar] [CrossRef]
- Barth, A. Infrared spectroscopy of proteins. Biochim. Biophys. Acta Bioenerg. 2007, 1767, 1073–1101. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sett, A.; Gadewar, M.; Sharma, P.; Deka, M.; Bora, U. Green synthesis of gold nanoparticles using aqueous extract of Dillenia indica. Adv. Nat. Sci. Nanosci. Nanotechnol. 2016, 7, 025005. [Google Scholar] [CrossRef]
- Ahluwalia, V.; Kumar, J.; Sisodia, R.; Shakil, N.A.; Walia, S. Green synthesis of silver nanoparticles by Trichoderma harzianum and their bio-efficacy evaluation against Staphylococcus aureus and Klebsiella pneumonia. Ind. Crops Prod. 2014, 55, 202–206. [Google Scholar] [CrossRef]
- Vidhu, V.K.; Philip, D. Catalytic degradation of organic dyes using biosynthesized silver nanoparticles. Micron 2014, 56, 54–62. [Google Scholar] [CrossRef]
- Aramwit, P.; Bang, N.; Ratanavaraporn, J.; Ekgasit, S. Green synthesis of silk sericin-capped silver nanoparticles and their potent anti-bacterial activity. Nanoscale Res. Lett. 2014, 9, 79. [Google Scholar] [CrossRef]
- Khan, M.M.R.; Tsukada, M.; Zhang, X.H.; Morikawa, H. Preparation and characterization of electrospun nanofibers based on silk sericin powders. J. Mater. Sci. 2013, 48, 3731–3736. [Google Scholar] [CrossRef]
- Pellati, F.; Brighenti, V.; Sperlea, J.; Marchetti, L.; Bertelli, D.; Benvenuti, S. New Methods for the Comprehensive Analysis of Bioactive Compounds in Cannabis sativa L. (hemp). Molecules 2018, 23, 2639. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Peschel, W.; Politi, M. H-1 NMR and HPLC/DAD for Cannabis sativa L. chemotype distinction, extract profiling and specification. Talanta 2015, 140, 150–165. [Google Scholar] [CrossRef] [PubMed]
- Drinic, Z.; Vladic, J.; Koren, A.; Zeremski, T.; Stojanov, N.; Kiprovski, B.; Vidovic, S. Microwave-assisted extraction of cannabinoids and antioxidants from Cannabis sativa aerial parts and process modeling. J. Chem. Technol. Biotechnol. 2020, 95, 831–839. [Google Scholar] [CrossRef]
- Moccia, S.; Siano, F.; Russo, G.L.; Volpe, M.G.; La Cara, F.; Pacifico, S.; Piccolella, S.; Picariello, G. Antiproliferative and antioxidant effect of polar hemp extracts (Cannabis sativa L., Fedora cv.) in human colorectal cell lines. Int. J. Food Sci. Nutr. 2019, 71, 410–423. [Google Scholar] [CrossRef]
- Protti, M.; Brighenti, V.; Battaglia, M.R.; Anceschi, L.; Pellati, F.; Mercolini, L. Cannabinoids from Cannabis sativa L.: A New Tool Based on HPLC DAD-MS/MS for a Rational Use in Medicinal Chemistry. ACS Med. Chem. Lett. 2019, 10, 539–544. [Google Scholar] [CrossRef]
- de Araujo, A.R.; Ramos-Jesus, J.; de Oliveira, T.M.; de Carvalho, A.M.A.; Nunes, P.H.M.; Daboit, T.C.; Carvalho, A.P.; Barroso, M.F.; de Almeida, M.P.; Placido, A.; et al. Identification of Eschweilenol C in derivative of Terminalia fagifolia Mart. and green synthesis of bioactive and biocompatible silver nanoparticles. Ind. Crops Prod. 2019, 137, 52–65. [Google Scholar] [CrossRef]
- Abdel-Aziz, M.S.; Shaheen, M.S.; El-Nekeety, A.A.; Abdel-Wahhab, M.A. Antioxidant and antibacterial activity of silver nanoparticles biosynthesized using Chenopodium murale leaf extract. J. Saudi Chem. Soc. 2014, 18, 356–363. [Google Scholar] [CrossRef] [Green Version]
- Carbonera, F.; Montanher, P.F.; Palombini, S.V.; Maruyama, S.A.; Claus, T.; Santos, H.M.C.; Sargi, S.C.; Matsushita, M.; Visentainer, J.V. Antioxidant capacity in tilapia fillets enriched with extract of acerola fruit residue. J. Braz. Chem. Soc. 2014, 25, 1237–1245. [Google Scholar] [CrossRef]
- Dawidowicz, A.L.; Olszowy, M. The importance of solvent type in estimating antioxidant properties of phenolic compounds by ABTS assay. Eur. Food Res. Technol. 2013, 236, 1099–1105. [Google Scholar] [CrossRef] [Green Version]
- Burlacu, E.; Tanase, C.; Coman, N.A.; Berta, L. A Review of Bark-Extract-Mediated Green Synthesis of Metallic Nanoparticles and Their Applications. Molecules 2019, 24, 4354. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- El-Seedi, H.R.; El-Shabasy, R.M.; Khalifa, S.A.M.; Saeed, A.; Shah, A.; Shah, R.; Iftikhar, F.J.; Abdel-Daim, M.M.; Omri, A.; Hajrahand, N.H.; et al. Metal nanoparticles fabricated by green chemistry using natural extracts: Biosynthesis, mechanisms, and applications. RSC Adv. 2019, 9, 24539–24559. [Google Scholar] [CrossRef] [Green Version]
- Chauhan, A.; Verma, R.; Kumari, S.; Sharma, A.; Shandilya, P.; Li, X.; Batoo, K.M.; Imran, A.; Kulshrestha, S.; Kumar, R. Photocatalytic Dye Degradation and Antimicrobial Activities of Pure and Ag-doped ZnO Using Cannabis Sativa Leaf Extract. Sci. Rep. 2020, 10, 7881. [Google Scholar] [CrossRef] [PubMed]
- Mosselhy, D.A.; El-Aziz, M.A.; Hanna, M.; Ahmed, M.A.; Husien, M.M.; Feng, Q. Comparative synthesis and antimicrobial action of silver nanoparticles and silver nitrate. J. Nanopart. Res. 2015, 17, 473. [Google Scholar] [CrossRef]
- Brighenti, V.; Pellati, F.; Steinbach, M.; Maran, D.; Benvenuti, S. Development of a new extraction technique and HPLC method for the analysis of non-psychoactive cannabinoids in fibre-type Cannabis sativa L. (hemp). J. Pharm. Biomed. Anal. 2017, 143, 228–236. [Google Scholar] [CrossRef]
- Moisa, C.; Copolovici, L.; Bungau, S.; Pop, G.; Imbrea, I.; Lupitu, A.; Nemeth, S.; Copolovici, D. Wastes Resulting from Aromatic Plants Distillation—Bio-Sources of Antioxidants and Phenolic Compounds with Biological Active Principles. Farmacia 2018, 66, 289–295. [Google Scholar]
- Csakvari, A.C.; Lupitu, A.; Bungau, S.; Gitea, M.A.; Gitea, D.; Tit, D.M.; Copolovici, L.; Nemeth, S.; Copolovici, D. Fatty Acids Profile and Antioxidant Activity of Almond Oils Obtained from Six Romanian Varieties. Farmacia 2019, 67, 882–887. [Google Scholar] [CrossRef] [Green Version]
- Sridhar, K.; Charles, A.L. In vitro antioxidant activity of Kyoho grape extracts in DPPH and ABTS assays: Estimation methods for EC50 using advanced statistical programs. Food Chem. 2019, 275, 41–49. [Google Scholar] [CrossRef] [PubMed]
Compound | CsDi | CsDe | CsSf | CsSm |
---|---|---|---|---|
Phenollic Compounds | ||||
Pyrogallol (mg L−1) | 0.44 ± 0.03 | 0.39 ± 0.03 | 0.67 ± 0.02 | 0.35 ± 0.04 |
Gallic acid (mg L−1) | 118.91 ± 0.71 | 95.46 ± 5.42 | 137.30 ± 9.85 | 96.14 ± 4.79 |
Quercetin (mg L−1) | 28.60 ± 0.39 | 35.14 ± 0.09 | 28.15 ± 0.15 | 24.26 ± 0.20 |
Kaempferol (mg L−1) | 2.07 ± 0.06 | 5.93 ± 0.14 | 0.39 ± 0.01 | 1.71 ± 0.03 |
Cannabinoid Acids | ||||
Cannabidioloc acid (CBDA) (%) | 0 | 0.037 ± 0.001 | 0.006 ± 0.008 | 0.195 ± 0.009 |
cannabigerolic acid (CBGA) (%) | 0.086 ± 0.019 | 0.104 ± 0.004 | 0.945 ± 0.006 | 0.052 ± 0.015 |
TPC (mg GAE L−1) | CsDi | CsDe | CsSf | CsSm |
---|---|---|---|---|
TPC1: Aqueous extract | 1601.09 ± 0.5 a | 1663.97 ± 0.17 b | 1721.79 ± 0.26 c | 1581.86 ± 0.34 d |
TPC2: Solution remained after the biosynthesis of AgNPs | 212.44 ± 0.28 a | 241.54 ± 0.48 b | 212.82 ± 0.36 a | 213.91 ± 0.32 c |
TPC1: TPC2 | 7.55 | 6.9 | 8.11 | 7.42 |
Scheme | Sample Name | ABTS•+ Assay | DPPH• Assay | ||
---|---|---|---|---|---|
Inhibition % | mmol TEAC/L | Inhibition % | mg GAE/L | ||
Plant extracts | CsDi | 66.293 ± 0.019 a | 0.232 ± 0.001 | 15.213 ± 0.058 a | 9.426 ± 0.031 |
CsDe | 80.076 ± 0.011 b | 0.611 ± 0.001 | 16.808 ± 0.052 b | 10.282 ± 0.028 | |
CsSm | 84.322 ± 0.139 c | 0.727 ± 0.004 | 11.820 ± 0.039 c | 7.606 ± 0.021 | |
CsSf | 81.973 ± 0.014 d | 0.663 ± 0.001 | 29.923 ± 0.002 d | 17.318 ± 0.001 | |
Extracts + AgNPs | PDi | 86.065 ± 0.026 a | 0.775 ± 0.001 | 3.398 ± 0.065 a | 3.087 ± 0.035 |
PDe | 88.523 ± 0.037 b | 0.843 ± 0.001 | 1.560 ± 0.035 b | 2.101 ± 0.019 | |
PSm | 89.185 ± 0.039 c | 0.861 ± 0.001 | 1.800 ± 0.007 c | 2.230 ± 0.004 | |
PSf | 90.334 ± 0.047 d | 0.893 ± 0.001 | 2.323 ± 0.067 d | 2.510 ± 0.036 | |
AgNPs | NPDi | 88.772 ± 0.044 a | 0.850 ± 0.001 | 5.604 ± 0.009 a | 4.271 ± 0.005 |
NPDe | 89.529 ± 0.017 b | 0.871 ± 0.001 | 6.651 ± 0.026 ab | 4.833 ± 0.014 | |
NPSm | 88.228 ± 0.017 c | 0.835 ± 0.001 | 6.670 ± 0.012 ab | 4.843 ± 0.007 | |
NPSf | 73.109 ± 0.025 d | 0.419 ± 0.001 | 6.383 ± 0.007 b | 4.689 ± 0.004 |
FT-IR Range(cm−1) | Main FT-IR Bands (cm−1) | |||||||
---|---|---|---|---|---|---|---|---|
CsDi | CsDe | CsSf | CsSm | PDi | PDe | PSf | PSm | |
3550–3100 | 3290.91 | 3296.60 | 3280.63 | 3286.07 | 3326.57 | 3262.02 | 3253.32 | 3252.98 |
3100–2850 | 2924.22 | 2926.59 | 2925.92 | 2925.24 | 2925.64 | 2931.89 | 2929.99 | 2930.31 |
2888.83 | 2898.46 | 2897.24 | 2899.24 | 2884.24 | 2899.38 | 2898.82 | 2898.72 | |
2859.24 | 2864.85 | 2859.74 | 2856.32 | 2858.99 | 2861.87 | 2858.78 | 2859.16 | |
1700–1500 | 1637.94 | 1637.02 | 1639.24 | 1638.92 | 1636.24 | 1635.92 | 1638.26 | 1637.13 |
1500–1200 | 1404.76 | 1407.54 | 1405.00 | 1405.01 | - | - | - | - |
- | - | - | - | 1368.33 | 1368.57 | 1368.01 | 1368.52 | |
1200–900 | 1043.20 | 1043.82 | 1043.13 | 1043.80 | 1043.19 | 1043.91 | 1043.20 | 1043.68 |
900–600 | 922.38 | 923.88 | 922.37 | 924.14 | 922.12 | 924.21 | 923.27 | 923.73 |
866.29 | 870.02 | 872.20 | 870.01 | 866.16 | 870.96 | 872.05 | 870.77 | |
778.69 | 822.41 | 825.41 | 825.80 | 824.24 | 824.71 | 824.44 | 824.82 | |
778.69 | 777.53 | 779.38 | 780.17 | 779.11 | 779.87 | 781.58 | 781.07 | |
712.03 | 712.32 | 712.01 | 713.15 | 711.83 | 711.47 | 9712.21 | 711.55 | |
672.59 | 669.25 | 669.25 | 670.55 | 672.28 | 668.35 | 667.45 | 675.57 | |
616.24 | 626.27 | 621.84 | 618.38 | 617.04 | 628.33 | 621.54 | 612.38 |
Sample Type | Sample Name | Z-Ave [nm] | PdI | Peak 1: d [nm] (Weight (%)) | Peak 2: d [nm] (Weight (%)) | Peak 3: d [nm] (Weight (%)) |
---|---|---|---|---|---|---|
Extracts + AgNPs | PDi | 79.8 | 0.371 | 104.3 (71%) | 33.9 (17%) | 3416 (12%) |
PDe | 139.9 | 0.424 | 266.0 (62%) | 74.2 (38%) | 0 | |
PSm | 119.2 | 0.367 | 198.8 (62%) | 75.5 (38%) | 0 | |
PSf | 102.1 | 0.376 | 180.8 (55%) | 75.1 (45%) | 0 | |
AgNPs | NPDi | 147.1 | 0.569 | 258.6 (66%) | 74.5 (31%) | 5337 (3%) |
NPDe | 113.7 | 0.338 | 156.8 (95%) | 32.1 (5%) | 0 | |
NPSm | 195.1 | 0.478 | 133.7 (59%) | 872.5 (41%) | 0 | |
NPSf | 104.3 | 0.293 | 77.2 (52%) | 210.4 (48%) | 0 |
Inhibition Zones Diameter (mm) | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Strain Name | CsDi | CsDe | CsSf | CsSm | PDi | PDe | PSf | PSm | Amikacin | Gentamicin |
K. pneumoniae | 6 | 7 ± 0.1 | 7 ± 0.17 | 8 ± 0.1 | 12 ± 0.17 | 14 ± 0.17 | 10 ± 0.0 | 12 ± 0.26 | 14 ± 0.2 | ND |
P. fluorescens | 6 | 6 | 6 | 6 | 11 ± 0.2 | 10 ± 0.2 | 11 ± 0.1 | 13 ± 0.17 | ND | 20 ± 0.1 |
E. coli | 7 ± 0.1 | 7 ± 0.1 | 7 ± 0.2 | 8 ± 0.1 | 10 ± 0.1 | 11 ± 0.26 | 12 ± 0.26 | 10 ± 0.1 | 20 ± 0.17 | ND |
S. aureus | 7 ± 0.1 | 7 ± 0.1 | 7 ± 0.1 | 7 ± 0.1 | 12 ± 0.17 | 13 ± 0.1 | 13 ± 0.0 | 13 ± 0.1 | ND | 21 ± 0.1 |
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Csakvari, A.C.; Moisa, C.; Radu, D.G.; Olariu, L.M.; Lupitu, A.I.; Panda, A.O.; Pop, G.; Chambre, D.; Socoliuc, V.; Copolovici, L.; et al. Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts. Molecules 2021, 26, 4041. https://doi.org/10.3390/molecules26134041
Csakvari AC, Moisa C, Radu DG, Olariu LM, Lupitu AI, Panda AO, Pop G, Chambre D, Socoliuc V, Copolovici L, et al. Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts. Molecules. 2021; 26(13):4041. https://doi.org/10.3390/molecules26134041
Chicago/Turabian StyleCsakvari, Adriana Cecilia, Cristian Moisa, Dana G. Radu, Leonard M. Olariu, Andreea I. Lupitu, Anca Ofelia Panda, Georgeta Pop, Dorina Chambre, Vlad Socoliuc, Lucian Copolovici, and et al. 2021. "Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts" Molecules 26, no. 13: 4041. https://doi.org/10.3390/molecules26134041