Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes
Abstract
:1. Introduction
2. Results
2.1. Characterization of L1–L2 and Their Zn2+ Complexes
2.1.1. Speciation in Solution of L1–L2
2.1.2. QM Modeling of the Zn2+ Complexes
2.2. Interaction Studies of Ligands to Double-Stranded Nucleic Acids
2.2.1. Thermal Denaturation Experiments
2.2.2. Fluorescent Indicator Displacement Assays
EB Displacement Assays
DAPI Displacement Assays
2.2.3. Molecular Modeling
2.3. Metal-Induced Modulation of RNA Binding
2.3.1. Thermal Denaturation Experiments of Metal Complexes
2.3.2. Fluorescence Displacement Studies of Metal Complexes
2.3.3. Molecular Modeling
2.4. Viability Assays of the Ligands and the Metal Complexes
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Synthesis of Ligands
4.3. EMF Measurements
4.4. Thermal Melting Experiments
4.5. Fluorescent Indicator Displacement Assays
4.6. Theoretical Studies
4.7. MTT Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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EB | DAPI | ||
---|---|---|---|
L1 | ctDNA | 1.37 | |
poly A-poly U | 0.63 | 0.85 | |
L2 | ctDNA | 2.03 | |
poly A-poly U | 0.86 | 0.45 |
Free Ligand | 1:1 (Zn2+–L) | 2:1 (Zn2+–L) | |
---|---|---|---|
L1 | 21.3 | 1.9 | 1.1 |
L2 | 17.1 | 4.1 | −1.1 |
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Guijarro, L.; Martínez-Camarena, Á.; Chicote, J.U.; García-España, A.; García-España, E.; Inclán, M.; Verdejo, B.; González-García, J. Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes. Molecules 2021, 26, 3957. https://doi.org/10.3390/molecules26133957
Guijarro L, Martínez-Camarena Á, Chicote JU, García-España A, García-España E, Inclán M, Verdejo B, González-García J. Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes. Molecules. 2021; 26(13):3957. https://doi.org/10.3390/molecules26133957
Chicago/Turabian StyleGuijarro, Lluís, Álvaro Martínez-Camarena, Javier U. Chicote, Antonio García-España, Enrique García-España, Mario Inclán, Begoña Verdejo, and Jorge González-García. 2021. "Ditopic Aza-Scorpiand Ligands Interact Selectively with ds-RNA and Modulate the Interaction upon Formation of Zn2+ Complexes" Molecules 26, no. 13: 3957. https://doi.org/10.3390/molecules26133957