Study on Absorption Mechanism and Tissue Distribution of Fucoidan
Abstract
:1. Introduction
2. Results
2.1. Fluorescence Labeling of Fucoidan
2.2. Establishment and Assessment of Seven-Day Absorption Model of Caco-2 Cells
2.3. Verification of the Absorption and Transport Function of Caco-2 Monolayer Cell Model
2.4. The Mechanism of Fucoidan Absorption and Transport
2.4.1. Absorption and Transport of Fucoidan
2.4.2. Effect of Inhibition of Clathrin-Mediated Endocytosis on the Absorption and Transport of Fucoidan
2.5. Tissue Distribution of Fucoidan in Mice
2.5.1. Toxicity of Fucoidan in Mice
2.5.2. Tissue Distribution of Fucoidan
3. Materials and Methods
3.1. Materials
3.2. Fluorescent Labeling of Fucoidan
3.3. Effect of FITC Labeling on the Content of Fucose and Sulfate
3.4. MTT Assay for Cell Viability
3.5. Caco-2 Cell Culture and Establishment of Caco-2 Monolayer Cell Model
3.6. Verification of the Absorption and Transport Function of Caco-2 Monolayer Cell Model
3.7. The Mechanism of Fucoidan Absorption and Transport
3.8. Tissue distribution of Fucoidan in Mice
3.9. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the fucoidan are available from the authors. |
Fucoidan | Content% | |
---|---|---|
Before Labeled | After Labeled | |
Fucose content | 42.86 ± 0.24 | 45.11 ± 0.88 |
Sulfate content | 25.37 ± 0.25 | 25.33 ± 0.34 |
Tissue | Standard Curve | R2 |
---|---|---|
Blood | Y = 1028.62 X + 48217.63 | 0.999 |
Heart | Y = 1595.96 X + 153165.43 | 0.994 |
Liver | Y = 3217.36 X + 164583.03 | 0.998 |
Spleen | Y = 2902.54 X + 26586.43 | 0.998 |
Pulmonary | Y = 3194.69 X + 35547.02 | 0.995 |
Renal | Y = 1791.22 X + 215822.65 | 0.99 |
Brain | Y = 2427.88 X + 48044.49 | 0.998 |
Parameter | Unit | Tissue | ||||
---|---|---|---|---|---|---|
Blood | Liver | Spleen | Lung | Kidney | ||
Kel | 1/h | 0.25 ± 0.10 | 0.07 ± 0.01 | 0.003 ± 0.02 | 0.18 ± 0.05 | 0.03 ± 0.04 |
T1/2 | h | 2.77 ± 0.82 | 10.67 ± 3.73 | 209.22 ± 6.94 | 3.79 ± 0.97 | 22.27 ± 1.75 |
Tmax | h | 0.5 | 0.5 ± 0.29 | 6 ± 1.15 | 4 ± 1 | 0.5 |
Cmax | μg/g | 66.37 ± 25.56 | 284.27 ± 211.88 | 77.79 ± 30.05 | 110.92 ± 81.897 | 1092.31 ± 297.66 |
C0 | μg/g | 135.28 ± 59.91 | 495.25 ± 159.14 | 47.61 ± 17.28 | 188.58 ± 48.32 | 1949.94 ± 1448.45 |
AUC 0-t | μg/g×h | 138.71 ± 20.64 | 1653.86 ± 567.04 | 1597.28 ± 394.38 | 1694.21 ± 580.70 | 7520.11 ± 2110.44 |
AUC 0-∞ | μg/g×h | 198.11 ± 41.10 | 2947.506 ± 992.52 | 22886.97 ± 1301.13 | 1709.85 ± 588.22 | 12834.30 ± 5247.13 |
MRT 0-∞ | h | 3.23 ± 1.30 | 14.66 ± 5.94 | 303.96 ± 13.44 | 6.88 ± 0.37 | 28.14 ± 12.324 |
CL | (mg)/(μg/g)/h | 0.25 ± 0.04 | 0.02 ± 0.001 | 0.002 ± 0.01 | 0.03 ± 0.004 | 0.004 ± 0.003 |
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Bai, X.; Zhang, E.; Hu, B.; Liang, H.; Song, S.; Ji, A. Study on Absorption Mechanism and Tissue Distribution of Fucoidan. Molecules 2020, 25, 1087. https://doi.org/10.3390/molecules25051087
Bai X, Zhang E, Hu B, Liang H, Song S, Ji A. Study on Absorption Mechanism and Tissue Distribution of Fucoidan. Molecules. 2020; 25(5):1087. https://doi.org/10.3390/molecules25051087
Chicago/Turabian StyleBai, Xu, E Zhang, Bo Hu, Hao Liang, Shuliang Song, and Aiguo Ji. 2020. "Study on Absorption Mechanism and Tissue Distribution of Fucoidan" Molecules 25, no. 5: 1087. https://doi.org/10.3390/molecules25051087