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Article

Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair

1
Department of Pharmacy, Health and Nutritional Sciences, Department of Excellence 2018–2022, University of Calabria, Edificio Polifunzionale, 87036 Rende (CS), Italy
2
Department of Chemistry and Chemical Technologies, University of Calabria, 87036 Rende (CS), Italy
*
Author to whom correspondence should be addressed.
Equally contributed.
Molecules 2020, 25(4), 968; https://doi.org/10.3390/molecules25040968
Received: 23 January 2020 / Revised: 18 February 2020 / Accepted: 18 February 2020 / Published: 21 February 2020
(This article belongs to the Section Applied Chemistry)
Glucans possess a broad spectrum of biological activities. In this context, the present study was performed to isolate glucans from an Italian cultivar of Ziziphus jujuba Mill. at three different harvesting periods, in order to evaluate their effects on wound healing. The dry fruits were subjected to an alkaline extraction and then isolated glucans were purified by dialyzation. The crude and soluble samples were characterized by FT-IR and SEM analyses. Afterwards, total, α- and β-glucan content was measured using an enzymatic procedure. The results highlighted that the glucan amount increased as the maturation proceeded as well as the β-glucan percentage, which ranged from 48.2 at the first harvesting to 65.4 at the third harvesting. Furthermore, the effects of isolated glucans on the viability and migration of keratinocytes were evaluated using the in vitro MTT and scratch wound assays. The best proliferative effects on keratinocyte migration have been achieved with soluble glucans from third harvesting at 100 μM after 24 and 48 h (*** P < 0.001). The same treated group showed significant narrowing of the scratch area after 24 h and complete closure of the injury after 48 h. The findings highlighted the effectiveness of soluble glucans on regeneration of damaged skin. View Full-Text
Keywords: Ziziphus jujuba Mill.; glucans; scanning electron microscopy; keratinocytes; MTT assay; scratch assay; wound healing Ziziphus jujuba Mill.; glucans; scanning electron microscopy; keratinocytes; MTT assay; scratch assay; wound healing
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MDPI and ACS Style

Fazio, A.; La Torre, C.; Caroleo, M.C.; Caputo, P.; Plastina, P.; Cione, E. Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair. Molecules 2020, 25, 968. https://doi.org/10.3390/molecules25040968

AMA Style

Fazio A, La Torre C, Caroleo MC, Caputo P, Plastina P, Cione E. Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair. Molecules. 2020; 25(4):968. https://doi.org/10.3390/molecules25040968

Chicago/Turabian Style

Fazio, Alessia, Chiara La Torre, Maria C. Caroleo, Paolino Caputo, Pierluigi Plastina, and Erika Cione. 2020. "Isolation and Purification of Glucans from an Italian Cultivar of Ziziphus jujuba Mill. and In Vitro Effect on Skin Repair" Molecules 25, no. 4: 968. https://doi.org/10.3390/molecules25040968

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