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Article

AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism

1
Saint Petersburg Institute of Bioregulation and Gerontology, Dynamo Ave., 3, 197110 St. Petersburg, Russia
2
Pavlov Institute of Physiology Russian Academy of Sciences, Makarova Emb., 6, 199034 St. Petersburg, Russia
3
Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, Italy
4
Academy of postgraduate education under FSBU FSCC of FMBA of Russia, Volokolamskaya r., 91, 125371 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Elio Pizzo
Molecules 2020, 25(3), 609; https://doi.org/10.3390/molecules25030609
Received: 1 December 2019 / Revised: 23 January 2020 / Accepted: 29 January 2020 / Published: 30 January 2020
(This article belongs to the Special Issue Chemical Functionalization of Bioactive Peptides)
It was shown that AEDG peptide (Ala-Glu-Asp-Gly, Epitalon) regulates the function of the pineal gland, the retina, and the brain. AEDG peptide increases longevity in animals and decreases experimental cancerogenesis. AEDG peptide induces neuronal cell differentiation in retinal and human periodontal ligament stem cells. The aim of the study was to investigate the influence of AEDG peptide on neurogenic differentiation gene expression and protein synthesis in human gingival mesenchymal stem cells, and to suggest the basis for the epigenetic mechanism of this process. AEDG peptide increased the synthesis of neurogenic differentiation markers: Nestin, GAP43, β Tubulin III, Doublecortin in hGMSCs. AEDG peptide increased Nestin, GAP43, β Tubulin III and Doublecortin mRNA expression by 1.6–1.8 times in hGMSCs. Molecular modelling method showed, that AEDG peptide preferably binds with H1/6 and H1/3 histones in His-Pro-Ser-Tyr-Met-Ala-His-Pro-Ala-Arg-Lys and Tyr-Arg-Lys-Thr-Gln sites, which interact with DNA. These results correspond to previous experimental data. AEDG peptide and histones H1/3, H1/6 binding may be one of the mechanisms which provides an increase of Nestin, GAP43, β Tubulin III, and Doublecortin neuronal differentiation gene transcription. AEDG peptide can epigenetically regulate neuronal differentiation gene expression and protein synthesis in human stem cells. View Full-Text
Keywords: AEDG peptide (Ala-Glu-Asp-Gly, Epitalon); human gingival mesenchymal stem cells; neurogenic differentiation; histones; epigenetic AEDG peptide (Ala-Glu-Asp-Gly, Epitalon); human gingival mesenchymal stem cells; neurogenic differentiation; histones; epigenetic
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MDPI and ACS Style

Khavinson, V.; Diomede, F.; Mironova, E.; Linkova, N.; Trofimova, S.; Trubiani, O.; Caputi, S.; Sinjari, B. AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism. Molecules 2020, 25, 609. https://doi.org/10.3390/molecules25030609

AMA Style

Khavinson V, Diomede F, Mironova E, Linkova N, Trofimova S, Trubiani O, Caputi S, Sinjari B. AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism. Molecules. 2020; 25(3):609. https://doi.org/10.3390/molecules25030609

Chicago/Turabian Style

Khavinson, Vladimir, Francesca Diomede, Ekaterina Mironova, Natalia Linkova, Svetlana Trofimova, Oriana Trubiani, Sergio Caputi, and Bruna Sinjari. 2020. "AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism" Molecules 25, no. 3: 609. https://doi.org/10.3390/molecules25030609

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