Next Article in Journal
Analysis of Binding Interactions of Ramipril and Quercetin on Human Serum Albumin: A Novel Method in Affinity Evaluation
Previous Article in Journal
Synthesis, Biological Assessment, and Structure Activity Relationship Studies of New Flavanones Embodying Chromene Moieties
Previous Article in Special Issue
Lipase-Catalyzed Chemoselective Ester Hydrolysis of Biomimetically Coupled Aryls for the Synthesis of Unsymmetric Biphenyl Esters
Open AccessArticle

Fine Modulation of the Catalytic Properties of Rhizomucor miehei Lipase Driven by Different Immobilization Strategies for the Selective Hydrolysis of Fish Oil

1
Bioprocess Engineering Department, Institute of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran 1497716316, Iran
2
Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran 1936773493, Iran
3
‘Nanobiotechnology for Life Sciences’ Group, Department of Chemistry in Pharmaceutical Sciences Dept., Faculty of Pharmacy, Universidad Complutense de Madrid (UCM), Plaza Ramón y Cajal s/n, E-28040 Madrid, Spain
4
Department of Biocatalysis, Institute of Catalysis, CSIC, 28049 Madrid, Spain
5
Departamento de Biotecnología y Microbiología de los Alimentos, Instituto de Alimentación (CIAL), CSIC-UAM, Madrid, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Dimitra Hadjipavlou-Litina
Molecules 2020, 25(3), 545; https://doi.org/10.3390/molecules25030545
Received: 17 December 2019 / Revised: 20 January 2020 / Accepted: 22 January 2020 / Published: 27 January 2020
(This article belongs to the Special Issue Lipases and Lipases Modification 2019)
Functional properties of each enzyme strictly depend on immobilization protocol used for linking enzyme and carrier. Different strategies were applied to prepare the immobilized derivatives of Rhizomucor miehei lipase (RML) and chemically aminated RML (NH2-RML). Both RML and NH2-RML forms were covalently immobilized on glyoxyl sepharose (Gx-RML and Gx-NH2-RML), glyoxyl sepharose dithiothreitol (Gx-DTT-RML and Gx-DTT-NH2-RML), activated sepharose with cyanogen bromide (CNBr-RML and CNBr-NH2-RML) and heterofunctional epoxy support partially modified with iminodiacetic acid (epoxy-IDA-RML and epoxy-IDA-NH2-RML). Immobilization varied from 11% up to 88% yields producing specific activities ranging from 0.5 up to 1.9 UI/mg. Great improvement in thermal stability for Gx-DTT-NH2-RML and epoxy-IDA-NH2-RML derivatives was obtained by retaining 49% and 37% of their initial activities at 70 °C, respectively. The regioselectivity of each derivative was also examined in hydrolysis of fish oil at three different conditions. All the derivatives were selective between cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and cis-4,7,10,13,16,19-docosahexaenoic acid (DHA) in favor of EPA. The highest selectivity (32.9 folds) was observed for epoxy-IDA-NH2-RML derivative in the hydrolysis reaction performed at pH 5 and 4 °C. Recyclability study showed good capability of the immobilized biocatalysts to be used repeatedly, retaining 50–91% of their initial activities after five cycles of the reaction. View Full-Text
Keywords: lipase immobilization; Rhizomucor miehei lipase; oriented immobilization; protein chemical modification; fish oil hydrolysis; omega-3 polyunsaturated fatty acids lipase immobilization; Rhizomucor miehei lipase; oriented immobilization; protein chemical modification; fish oil hydrolysis; omega-3 polyunsaturated fatty acids
Show Figures

Figure 1

MDPI and ACS Style

Yousefi, M.; Marciello, M.; Guisan, J.M.; Fernandez-Lorente, G.; Mohammadi, M.; Filice, M. Fine Modulation of the Catalytic Properties of Rhizomucor miehei Lipase Driven by Different Immobilization Strategies for the Selective Hydrolysis of Fish Oil. Molecules 2020, 25, 545.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop