Next Article in Journal
Editorial to the Special Issue “Total Synthesis of Natural Products: A Themed Issue Dedicated to Professor Dr. Dieter Schinzer for His 65th Birthday”
Previous Article in Journal
Theoretical Prediction on the New Types of Noble Gas Containing Anions OBONgO and OCNNgO (Ng=He, Ar, Kr and Xe)
Previous Article in Special Issue
Application of Humanized Zebrafish Model in the Suppression of SARS-CoV-2 Spike Protein Induced Pathology by Tri-Herbal Medicine Coronil via Cytokine Modulation
Article

Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays

1
Graduate School of Regional Innovation Studies, Mie University, Tsu 514-8507, Japan
2
Zebrafish Drug Screening Center, Mie University, Tsu 514-8507, Japan
3
Rohto Pharmaceutical Co., Ltd, Osaka 544-0012, Japan
4
Department of Integrative Pharmacology, Mie University Graduate School of Medicine, Tsu 514-8507, Japan
5
Department of Bioinformatics, Mie University Advanced Science Research Promotion Center, Tsu 514-8507, Japan
*
Author to whom correspondence should be addressed.
These authors equally contributed to the work.
Academic Editor: Mark von Itzstein
Molecules 2020, 25(24), 5840; https://doi.org/10.3390/molecules25245840
Received: 28 September 2020 / Revised: 15 November 2020 / Accepted: 10 December 2020 / Published: 10 December 2020
(This article belongs to the Special Issue Zebrafish-Based Drug Screening)
(1) Background: The obesity epidemic has been drastically progressing in both children and adults worldwide. Pharmacotherapy is considered necessary for its treatment. However, many anti-obesity drugs have been withdrawn from the market due to their adverse effects. Instead, natural products (NPs) have been studied as a source for drug discovery for obesity, with the goal of limiting the adverse effects. Zebrafish are ideal model animals for in vivo testing of anti-obesity NPs, and disease models of several types of obesity have been developed. However, the evidence for zebrafish as an anti-obesity drug screening model are still limited. (2) Methods: We performed anti-adipogenic testing using the juvenile zebrafish obesogenic test (ZOT) and mouse 3T3-L1 preadipocytes using the focused NP library containing 38 NPs and compared their results. (3) Results: Seven and eleven NPs reduced lipid accumulation in zebrafish visceral fat tissues and mouse adipocytes, respectively. Of these, five NPs suppressed lipid accumulation in both zebrafish and 3T3-L1 adipocytes. We confirmed that these five NPs (globin-digested peptides, green tea extract, red pepper extract, nobiletin, and Moringa leaf powder) exerted anti-obesity effects in diet-induced obese adult zebrafish. (4) Conclusions: ZOT using juvenile fish can be a high-throughput alternative to ZOT using adult zebrafish and can be applied for in vivo screening to discover novel therapeutics for visceral obesity and potentially also other disorders. View Full-Text
Keywords: drug discovery; diabetes; metabolic syndrome; screening drug discovery; diabetes; metabolic syndrome; screening
Show Figures

Figure 1

MDPI and ACS Style

Nakayama, H.; Hata, K.; Matsuoka, I.; Zang, L.; Kim, Y.; Chu, D.; Juneja, L.R.; Nishimura, N.; Shimada, Y. Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays. Molecules 2020, 25, 5840. https://doi.org/10.3390/molecules25245840

AMA Style

Nakayama H, Hata K, Matsuoka I, Zang L, Kim Y, Chu D, Juneja LR, Nishimura N, Shimada Y. Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays. Molecules. 2020; 25(24):5840. https://doi.org/10.3390/molecules25245840

Chicago/Turabian Style

Nakayama, Hiroko, Kanae Hata, Izumi Matsuoka, Liqing Zang, Youngil Kim, Djongchi Chu, Lekh R. Juneja, Norihiro Nishimura, and Yasuhito Shimada. 2020. "Anti-Obesity Natural Products Tested in Juvenile Zebrafish Obesogenic Tests and Mouse 3T3-L1 Adipogenesis Assays" Molecules 25, no. 24: 5840. https://doi.org/10.3390/molecules25245840

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop