Next Article in Journal
Chagas Disease: Perspectives on the Past and Present and Challenges in Drug Discovery
Previous Article in Journal
Antiproliferative Homoleptic and Heteroleptic Phosphino Silver(I) Complexes: Effect of Ligand Combination on Their Biological Mechanism of Action
Article

Anti-Quorum Sensing Activity of Stevia Extract, Stevioside, Rebaudioside A and Their Aglycon Steviol

1
Department of Medical Biochemistry, Faculty of Medicine, Near East University, Nicosia 99138, Cyprus
2
Avram and Stella Goldstein-Goren Department of Biotechnology Engineering, Ben-Gurion University of the Negev, Be’er Sheva 84105, Israel
3
Department of Biochemistry, Faculty of Pharmacy, Girne American University, Kyrenia 99428, Cyprus
4
The Ilse Katz Center for Meso and Nanoscale Science and Technology, Ben-Gurion University of the Negev, Be’er Sheva 84105, Israel
*
Authors to whom correspondence should be addressed.
Molecules 2020, 25(22), 5480; https://doi.org/10.3390/molecules25225480
Received: 6 November 2020 / Revised: 20 November 2020 / Accepted: 21 November 2020 / Published: 23 November 2020
Governments are creating regulations for consumers to reduce their sugar intake, prompting companies to increase the ratio of artificial sweeteners in their products. However, there is evidence of some deleterious effects ascribed to the aforementioned synthetic agents and therefore consumers and food manufacturers have turned their attention to natural dietary sweeteners, such as stevia, to meet their sweetening needs. Stevia is generally considered safe; however, emerging scientific evidence has implicated the agent in gut microbial imbalance. In general, regulation of microbial behavior is known to depend highly on signaling molecules via quorum sensing (QS) pathways. This is also true for the gut microbial community. We, therefore, evaluated the possible role of these stevia-based natural sweeteners on this bacterial communication pathway. The use of a commercial stevia herbal supplement resulted in an inhibitory effect on bacterial communication, with no observable bactericidal effect. Purified stevia extracts, including stevioside, rebaudioside A (Reb A), and steviol revealed a molecular interaction, and possible interruption of Gram-negative bacterial communication, via either the LasR or RhlR receptor. Our in-silico analyses suggest a competitive-type inhibitory role for steviol, while Reb A and stevioside are likely to inhibit LasR-mediated QS in a non-competitive manner. These results suggest the need for further safety studies on the agents. View Full-Text
Keywords: quorum sensing; inhibition; stevia extract; stevioside; rebaudioside A; steviol quorum sensing; inhibition; stevia extract; stevioside; rebaudioside A; steviol
Show Figures

Figure 1

MDPI and ACS Style

Markus, V.; Share, O.; Teralı, K.; Ozer, N.; Marks, R.S.; Kushmaro, A.; Golberg, K. Anti-Quorum Sensing Activity of Stevia Extract, Stevioside, Rebaudioside A and Their Aglycon Steviol. Molecules 2020, 25, 5480. https://doi.org/10.3390/molecules25225480

AMA Style

Markus V, Share O, Teralı K, Ozer N, Marks RS, Kushmaro A, Golberg K. Anti-Quorum Sensing Activity of Stevia Extract, Stevioside, Rebaudioside A and Their Aglycon Steviol. Molecules. 2020; 25(22):5480. https://doi.org/10.3390/molecules25225480

Chicago/Turabian Style

Markus, Victor, Orr Share, Kerem Teralı, Nazmi Ozer, Robert S. Marks, Ariel Kushmaro, and Karina Golberg. 2020. "Anti-Quorum Sensing Activity of Stevia Extract, Stevioside, Rebaudioside A and Their Aglycon Steviol" Molecules 25, no. 22: 5480. https://doi.org/10.3390/molecules25225480

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop