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Article

Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro

1
Department for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, Serbia
2
Department for Pharmaceutical Investigations and Development, Institute for Medicinal Plant Research, Dr. Josif Pančić, 11 070 Belgrade, Serbia
3
Department for Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, 11 221 Belgrade, Serbia
*
Author to whom correspondence should be addressed.
Academic Editors: Raffaele Pezzani and Sara Vitalini
Molecules 2020, 25(22), 5233; https://doi.org/10.3390/molecules25225233
Received: 1 September 2020 / Revised: 26 October 2020 / Accepted: 26 October 2020 / Published: 10 November 2020
(This article belongs to the Special Issue Biological and Pharmacological Activity of Plant Natural Compounds)
Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell–cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues. View Full-Text
Keywords: doxorubicin; Mahonia aquifolium; matrix metalloproteinases; cytotoxicity; human lung adenocarcinoma doxorubicin; Mahonia aquifolium; matrix metalloproteinases; cytotoxicity; human lung adenocarcinoma
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MDPI and ACS Style

Damjanović, A.; Kolundžija, B.; Matić, I.Z.; Krivokuća, A.; Zdunić, G.; Šavikin, K.; Janković, R.; Stanković, J.A.; Stanojković, T.P. Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. Molecules 2020, 25, 5233. https://doi.org/10.3390/molecules25225233

AMA Style

Damjanović A, Kolundžija B, Matić IZ, Krivokuća A, Zdunić G, Šavikin K, Janković R, Stanković JA, Stanojković TP. Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro. Molecules. 2020; 25(22):5233. https://doi.org/10.3390/molecules25225233

Chicago/Turabian Style

Damjanović, Ana, Branka Kolundžija, Ivana Z. Matić, Ana Krivokuća, Gordana Zdunić, Katarina Šavikin, Radmila Janković, Jelena A. Stanković, and Tatjana P. Stanojković 2020. "Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro" Molecules 25, no. 22: 5233. https://doi.org/10.3390/molecules25225233

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