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Article

Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum

1
Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
2
Department of Sciences, King Khalid Military Academy, Riyadh 11495, P.O. Box 22140, Saudi Arabia
3
Chemistry Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(20), 4822; https://doi.org/10.3390/molecules25204822
Received: 31 August 2020 / Revised: 5 October 2020 / Accepted: 15 October 2020 / Published: 20 October 2020
Penicillium chrysogenum has been reported as a potent taxol producer based on quantitative analysis by TLC and HPLC. The biosynthetic potency of taxol has been validated from PCR detection of rate-limiting genes of taxol synthesis such as taxadienesynthase and 10-de-acetylbaccatin III-O-acetyltransferase (DBAT), which catalyzes the immediate diterpenoid precursor of the taxol substance, as detected by PCR. Taxol production by P. chrysogenum was assessed by growing the fungus on different media. Potato dextrose broth (PDB) was shown to be the best medium for obtaining the higher amount of taxol (170 µg/L). A stepwise optimization of culture conditions necessary for production of higher amounts of taxol was investigated. The substance taxol was produced optimally after 18 d of incubation at 30 °C in PDB adjusted initially at pH 8.0 with shaking (120 rpm) (250 µg/L). The P. chrysogenum taxol was purified successfully by HPLC. Instrumental analyzes such as Fourier transform infrared spectroscopy (FTIR), ultraviolet (UV) spectroscopy, 1HNMR and 13C NMR approved the structural formula of taxol (C47H51NO14), as constructed by ChemDraw. The P. chrysogenum taxol showed promising anticancer activity. View Full-Text
Keywords: Penicillium chrysogenum; taxol; instrumental analysis; antiproliferative activity Penicillium chrysogenum; taxol; instrumental analysis; antiproliferative activity
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MDPI and ACS Style

El-Sayed, A.; Enan, G.; Al-Mohammadi, A.-R.; Moustafa, A.H.; El-Gazzar, N. Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum. Molecules 2020, 25, 4822. https://doi.org/10.3390/molecules25204822

AMA Style

El-Sayed A, Enan G, Al-Mohammadi A-R, Moustafa AH, El-Gazzar N. Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum. Molecules. 2020; 25(20):4822. https://doi.org/10.3390/molecules25204822

Chicago/Turabian Style

El-Sayed, Ashraf, Gamal Enan, Abdul-Raouf Al-Mohammadi, Ahmed H. Moustafa, and Nashwa El-Gazzar. 2020. "Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by Penicillium chrysogenum" Molecules 25, no. 20: 4822. https://doi.org/10.3390/molecules25204822

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