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Open AccessFeature PaperArticle

Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies

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Department of Pediatrics, Gastroenterology, Hepatology, Nutrition and Allergology, Medical University of Bialystok, 15-276 Bialystok, Poland
2
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA
3
Department of Allergology and Internal Medicine, Medical University of Bialystok, 15-276 Bialystok, Poland
4
Department of Experimental Allergology and Immunology, Medical University of Bialystok, 15-276 Bialystok, Poland
*
Authors to whom correspondence should be addressed.
Molecules 2020, 25(2), 369; https://doi.org/10.3390/molecules25020369
Received: 19 December 2019 / Revised: 8 January 2020 / Accepted: 13 January 2020 / Published: 16 January 2020
(This article belongs to the Section Macromolecular Chemistry)
Four recombinant (r) allergens (rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101) were successfully produced and used for sensitization studies. The allergens belong to the profilin family which is one of the most numerous allergen families. These four proteins represent allergens originating from pollen of weeds (rAmb a 8.0101 and rArt v 4.0101), tree (rBet v 2.0101) and grass (rPhl p 12.0101). The recombinant allergens were characterized using various biochemical and biophysical methods and tested for their ability to bind patient-derived antibodies. One hundred patients aged 2 to 50 years sensitized to pollen and plant-derived food allergens (IgE > 0.35 kU/L) were included. Sensitization to individual allergen sources and components of birch and timothy pollens was evaluated using multiparameter immunoblots. The presence of IgE to pollen-derived recombinant profilins rAmb a 8.0101, rArt v 4.0101, rBet v 2.0101, and rPhl p 12.0101 in serum was evaluated using ELISA method. The presence of IgE against pollen profilins was detected in 20 out of 100 studied patients. High correlation was seen between IgE ELISA results with individual pollen profilins. In summary, it was shown that the recombinant versions of the four allergenic profilins can be used for sensitization studies and for component-resolved allergy diagnostics. View Full-Text
Keywords: recombinant allergen; IgE; pollen-food allergy; profilin recombinant allergen; IgE; pollen-food allergy; profilin
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Cudowska, B.; Kapingidza, A.B.; Pawłowicz, M.; Pampuch, A.; Hyduke, N.; Pote, S.; Schlachter, C.R.; Lebensztejn, D.M.; Chruszcz, M.; Kowal, K. Production and Use of Recombinant Profilins Amb a 8, Art v 4, Bet v 2, and Phl p 12 for Allergenic Sensitization Studies. Molecules 2020, 25, 369.

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