Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines
Abstract
:1. Introduction
2. Results and Discussion
2.1. Extraction Yields of IPTF and OPTF
2.2. Total Triterpenoids in I. obliquus
2.3. Composition of Triterpenoids in I. obliquus
2.4. Anti-Proliferative Activity of Triterpenoid Fractions from I. obliquus
3. Materials and Methods
3.1. Chemicals and Reagents
3.2. Material
3.3. Extraction and Fractionation
3.4. Determination of Total Triterpenoids
3.5. Analysis of Triterpenoid Composition
3.6. Cell Culture
3.7. Anti-Proliferative Activity
3.8. Statistics
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Fraction | Yield (%, w/w) | Triterpenoid Content (mg UAE/g) | |
---|---|---|---|
Dry Basis of I. obliquus | Dry Basis of Fraction | Dry Basis of I. obliquus | |
IPTF | 0.51 ± 0.04 | 469.2 ± 10.2 | 2.51 ± 0.04 |
OPTF | 0.78 ± 0.14 * | 554.5 ± 13.9 * | 4.32 ± 0.11 * |
Peak No. a | Compound | Fraction (mg/g Dry Basis of Fraction) | |
---|---|---|---|
IPTF | OPTF | ||
1 | Betulinic acid | 8.69 ± 1.71 | 13.6 ± 2.29 * |
2 | Betulin | 8.24 ± 0.65 | 8.79 ± 0.40 |
3 | Trametenolic acid b | 94.5 ± 9.15 | 106.3 ± 8.23 |
4 | Inotodiol | 153.9 ± 15.4 | 194.1 ± 11.5 * |
5 | Unknown b | 59.0 ± 4.04 | 47.1 ± 15.7 |
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Kim, J.; Yang, S.C.; Hwang, A.Y.; Cho, H.; Hwang, K.T. Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines. Molecules 2020, 25, 4066. https://doi.org/10.3390/molecules25184066
Kim J, Yang SC, Hwang AY, Cho H, Hwang KT. Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines. Molecules. 2020; 25(18):4066. https://doi.org/10.3390/molecules25184066
Chicago/Turabian StyleKim, Jaecheol, Si Chang Yang, Ah Young Hwang, Hyunnho Cho, and Keum Taek Hwang. 2020. "Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines" Molecules 25, no. 18: 4066. https://doi.org/10.3390/molecules25184066