Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines
1
Department of Food and Nutrition, and Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea
2
Food Analysis Team, Busan Metropolitan City Institute of Health and Environment, Busan 46616, Korea
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(18), 4066; https://doi.org/10.3390/molecules25184066
Received: 18 August 2020
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Revised: 4 September 2020
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Accepted: 4 September 2020
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Published: 6 September 2020
(This article belongs to the Section Natural Products Chemistry)
The objective of this study was to determine the composition of triterpenoids in the extracts from the inner and outer parts of Inonotus obliquus and to evaluate their anti-proliferative activity against cancer cell lines (HT-29, AGS, MCF-7, and PC3). Inner and outer parts of I. obliquus were extracted with 80% methanol for 24 h. The extract was fractionated by Diaion HP-20 resin to obtain the triterpenoid fraction. Composition of triterpenoids in the fraction was analyzed by HPLC and LC-ESI-MS. Anti-proliferative activity was evaluated by MTT assay against cancer cell lines. Inotodiol and trametenolic acid were major triterpenoids in both of the inner and outer parts of I. obliquus. Inotodiol in triterpenoid fractions from the inner and outer parts of I. obliquus was 153.9 ± 15.4 mg/g (dry basis (db)) and 194.1 ± 11.5 mg/g, respectively. Trametenolic acid in triterpenoid fractions from the inner and outer parts of I. obliquus was 94.5 ± 9.15 mg/g (db) and 106.3 ± 8.23 mg/g, respectively. Triterpenoids in the outer part were significantly higher than those in the inner part. Anti-proliferative activity of the triterpenoid fraction from the outer part against AGS, MCF-7, and PC3 was also significantly higher than that of the inner part.
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Keywords:
Inonotus obliquus; chaga mushroom; triterpenoids; cancer cells; anti-proliferative activity
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MDPI and ACS Style
Kim, J.; Yang, S.C.; Hwang, A.Y.; Cho, H.; Hwang, K.T. Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines. Molecules 2020, 25, 4066. https://doi.org/10.3390/molecules25184066
AMA Style
Kim J, Yang SC, Hwang AY, Cho H, Hwang KT. Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines. Molecules. 2020; 25(18):4066. https://doi.org/10.3390/molecules25184066
Chicago/Turabian StyleKim, Jaecheol, Si C. Yang, Ah Y. Hwang, Hyunnho Cho, and Keum T. Hwang. 2020. "Composition of Triterpenoids in Inonotus obliquus and Their Anti-Proliferative Activity on Cancer Cell Lines" Molecules 25, no. 18: 4066. https://doi.org/10.3390/molecules25184066
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