The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics
1
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China
2
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2020, 25(18), 4038; https://doi.org/10.3390/molecules25184038
Received: 15 August 2020
/
Revised: 1 September 2020
/
Accepted: 3 September 2020
/
Published: 4 September 2020
Chlorogenic acid (CGA), a natural phenolic compound, is an important bioactive compound, and its antibacterial activity has been widely concerned, but its antibacterial mechanism remains largely unknown. Protein leakage and the solution exosmosis conductivity of Bacillus subtilis 24434 (B. subtilis) reportedly display no noticeable differences before and after CGA treatment. The bacterial cells treated with CGA displayed a consistently smooth surface under the electron microscope, indicating that CGA cannot directly disrupt bacterial membranes. However, CGA induced a significant decrease in the intracellular adenosine triphosphate (ATP) concentration, possibly by affecting the material and energy metabolism or cell-signaling transduction. Furthermore, metabolomic results indicated that CGA stress had a bacteriostatic effect by inducing the intracellular metabolic imbalance of the tricarboxylic acid (TCA) cycle and glycolysis, leading to metabolic disorder and death of B. subtilis. These findings improve the understanding of the complex action mechanisms of CGA antimicrobial activity and provide theoretical support for the application of CGA as a natural antibacterial agent.
View Full-Text
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Wu, Y.; Liang, S.; Zhang, M.; Wang, Z.; Wang, Z.; Ren, X. The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics. Molecules 2020, 25, 4038. https://doi.org/10.3390/molecules25184038
AMA Style
Wu Y, Liang S, Zhang M, Wang Z, Wang Z, Ren X. The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics. Molecules. 2020; 25(18):4038. https://doi.org/10.3390/molecules25184038
Chicago/Turabian StyleWu, Yan, Shan Liang, Min Zhang, Zhenhua Wang, Ziyuan Wang, and Xin Ren. 2020. "The Effect of Chlorogenic Acid on Bacillus subtilis Based on Metabolomics" Molecules 25, no. 18: 4038. https://doi.org/10.3390/molecules25184038
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
