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Open AccessArticle

Comparison of Stir Bar Sorptive Extraction and Solid Phase Microextraction of Volatile and Semi-Volatile Metabolite Profile of Staphylococcus Aureus

1
EA7352 CHROME, University of Nimes, Rue du Dr G. Salan, 30021 Nimes CEDEX 1, France
2
Institut National de la Santé et de la Recherche Médicale, U1047, Université Montpellier, UFR de Médecine, 30908 Nîmes, France
3
Department of Microbiology, CHU Nîmes, Université Montpellier, 30029 Nîmes, France
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(1), 55; https://doi.org/10.3390/molecules25010055
Received: 29 November 2019 / Revised: 14 December 2019 / Accepted: 16 December 2019 / Published: 23 December 2019
For the analysis of volatile bacterial compounds, solid phase microextraction (SPME) is currently the most widely used metabolite concentration technique. Recently, the potential of stir bar sorptive extraction (SBSE) for this use has been demonstrated. These two approaches were therefore used in combination with gas-chromatography coupled with mass-spectrometry (GC–MS) for the analysis of volatile and semi-volatile bacterial compounds produced by Staphylococcus aureus. In both cases, SPME and SBSE/headspace sorptive extraction (HSSE) enrichment was carried out in two coating phases. A whole analytical and statistical process was developed to differentiate the metabolites produced from the metabolites consumed. The results obtained with SBSE/HSSE and SPME were compared and showed the recovery of 90% of the compounds by SBSE/HSSE. In addition, we were able to detect the production of 12 volatile/semi-volatile compounds by S. aureus, six of which had never been reported before. The extraction by SBSE/HSSE showed higher concentration capacities and greater sensitivity than SPME concerning bacterial compounds, suggesting that this technique may therefore become the new preferred option for bacterial volatile and semi-volatile compound analysis. View Full-Text
Keywords: gas-chromatography–mass-spectrometry; stir bar sorptive extraction; solid phase microextraction; Staphylococcus aureus; bacterial metabolite analysis gas-chromatography–mass-spectrometry; stir bar sorptive extraction; solid phase microextraction; Staphylococcus aureus; bacterial metabolite analysis
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MDPI and ACS Style

Berrou, K.; Dunyach-Remy, C.; Lavigne, J.-P.; Roig, B.; Cadiere, A. Comparison of Stir Bar Sorptive Extraction and Solid Phase Microextraction of Volatile and Semi-Volatile Metabolite Profile of Staphylococcus Aureus. Molecules 2020, 25, 55. https://doi.org/10.3390/molecules25010055

AMA Style

Berrou K, Dunyach-Remy C, Lavigne J-P, Roig B, Cadiere A. Comparison of Stir Bar Sorptive Extraction and Solid Phase Microextraction of Volatile and Semi-Volatile Metabolite Profile of Staphylococcus Aureus. Molecules. 2020; 25(1):55. https://doi.org/10.3390/molecules25010055

Chicago/Turabian Style

Berrou, Kevin; Dunyach-Remy, Catherine; Lavigne, Jean-Philippe; Roig, Benoit; Cadiere, Axelle. 2020. "Comparison of Stir Bar Sorptive Extraction and Solid Phase Microextraction of Volatile and Semi-Volatile Metabolite Profile of Staphylococcus Aureus" Molecules 25, no. 1: 55. https://doi.org/10.3390/molecules25010055

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