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Article

Carpesium divaricatum Sieb. & Zucc. Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant

by
Natalia Kłeczek
1,
Barbara Michalak
2,
Janusz Malarz
1,
Anna Karolina Kiss
2 and
Anna Stojakowska
1,*
1
Institute of Pharmacology, Polish Academy of Sciences, Department of Phytochemistry, 31-343 Kraków, Smętna Street 12, Poland
2
Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 1 Banacha Street, 02-097 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(8), 1614; https://doi.org/10.3390/molecules24081614
Submission received: 29 March 2019 / Revised: 18 April 2019 / Accepted: 20 April 2019 / Published: 24 April 2019
(This article belongs to the Section Natural Products Chemistry)
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Abstract

Carpesium divaricatum Sieb. & Zucc. has a long history of use as both a medicinal and a food plant. However, except for terpenoids, its chemical constituents have remained poorly investigated. The composition of hydroalcoholic extract from aerial parts of C. divaricatum was analyzed by HPLC-DAD-MSn, revealing the presence of numerous caffeic acid derivatives that were formerly unknown constituents of the plant. In all, 17 compounds, including commonly found chlorogenic acids and rarely occurring butyryl and methylbutyryl tricaffeoylhexaric acids, were tentatively identified. Fractionation of lipophilic extract from cultivated shoots led to the isolation of 12-oxo-phytodienoic acid (12-OPDA), which is a newly identified constituent of the plant. The compound, at concentrations of 0.5, 1.0, and 2.5 μM, significantly reduced IL-8, IL-1β, TNFα, and CCL2 excretion by lipopolysaccharide (LPS)-stimulated human neutrophils. Reactive oxygen species (ROS) production induced by f-MLP was also significantly diminished in the neutrophils pretreated by 12-OPDA. The newly identified constituents of the plant seem to be partly responsible for its pharmacological activity and elevate the value of C. divaricatum as a potential functional food.

Graphical Abstract

1. Introduction

The genus Carpesium (Asteraceae, tribe Inuleae), following recent taxonomic studies, comprises 25 species distributed in Asia and southeastern Europe [1]. Plants of the genus, due to their anti-inflammatory, antipyretic, detoxifying, anti-oedematous haemostatic, and antimalarial properties, have long been used as traditional medicines in China, Japan, and Korea to treat various ailments [2,3]. Whole herbs or aerial parts of the plants are also eaten, by local communities, as a seasonal vegetable, functional food, or vitamin source [4,5]. Aerial parts of Carpesium plants are also used to prepare brewed beverages or serve as forage [2,5].
The whole plant of Carpesium divaricatum Sieb. & Zucc. has been used in traditional medicine of the Far East as a remedy in colds, fevers, diarrhea, infections of the urinary tract, sore throat, toothache, and ascaridiasis [3]. Phytochemical studies on specialized metabolites of the plant are almost entirely devoted to terpenoids, especially sesquiterpene lactones, which reportedly possess anti-inflammatory and cytostatic properties [3,6,7].
The aim of the present study was an identification of non-terpenoid constituents of the plant that may contribute to the activity of its preparations.

2. Results

2.1. Caffeic Acid Derivatives in Aerial Parts of C. divaricatum

HPLC-DAD-MSn analysis of hydroalcoholic extracts from aerial parts of the plant revealed the presence of 17 compounds with absorption maxima at 324–328 nm (typical of caffeic acid and its conjugates). No substantial peaks of compounds that demonstrated different UV/Vis spectral properties were observed (Figure 1). Peaks 1 and 2 (m/z = 353 [M − H]−) were easily identified as signals of 3-O-caffeoylquinic acid (3-CQA; IUPAC numbering system) and 5-O-caffeoylquinic acid (5-CQA), respectively, whilst compounds 4–6 and 8 (m/z = 515 [M − H]−), taking into consideration fragmentation patterns of their quasimolecular ions (Table 1), proved to be four different isomers of di-O-caffeoyl quinic acid (DCQA), namely: 3,4-; 1,5-; 3,5-; and 4,5-di-O-caffeoylquinic acids [8,9]. Peaks 3, 7, and 9, representing compounds that showed a cleavage of two or three caffeoyl [M – H − (2 − 3 × 162)]− moieties resulting in the m/z 209 fragment, were assigned to hexaric acid derivatives: di-O caffeoylhexaric acid (I) (peak 3), tri-O-caffeoylhexaric acid (I) (peak 7), and tri-O-caffeoylheharic acid (II) (peak 9). The compounds have been previously described as constituents of other plants of the Asteraceae family [10,11,12,13], including the systematically related species Inula helenium [14]. Compounds corresponding to peaks 10 and 11 were tentatively identified as isobutyryl-dicaffeoylquinic acids based on the presence of quasimolecular ions at m/z 585 [M − H]− and product ions at m/z 497 [585 − C3H7COOH]−, 423 [585 − caffeoyl]−, 335 [497 − caffeoyl]− or [423 − C3H7COOH]−, and 179 [caffeic acid − H]−, which is in accordance with the data given by Heilmann et al. [15]. The compound represented by peak 12 (m/z at 677 [M − H]−) and producing fragmentation ions at m/z 515 and 353 was tentatively identified as 3,4,5-tri-O-caffeoylquinic acid [16]. Compounds corresponding to signals 13 and 14 (m/z at 599 [M − H]−), which demonstrated similar fragmentation patterns to those of 10 and 11, except for the fact that the product ion at m/z 497 was generated by the cleavage of 2-methylbutyryl or 3-methylbutyryl (isovaleryl) instead of the isobutyryl moiety [599 − C4H9COOH]−, could easiest be envisioned as 2-methylbutyryl/isovaleryl-di-O-caffeoylquinic acids. The compound represented by the signal 15 was probably a deoxy derivative of leontopodic acid judging by the m/z value of its quasimolecular ion (765 [M − H]−) and fragmentation ions at m/z 603 and 441. The fragmentation pattern of leontopodic acid (m/z = 781 [M − H]−) exhibited fragment ions at m/z = 619 [M − H − caffeoyl]− and at m/z = 457 [M − H − 2 × caffeoyl]− [17]. Finally, the compounds corresponding to peaks 16 and 17, with molecular ion masses and fragmentation ion masses being 14 mass units higher than those of 15, could be tentatively identified as 2-methylbutyryl/isovaleryl-tri-O-caffeoylhexaric acids. The dry plant material contained 1.23 ± 0.006% of 5-CQA and 1.46 ± 0.023% of 3,5-DCQA.

2.2. Identification of Trans-12-Oxo-phytodienoic Acid (trans-12-OPDA)

Chromatographic separation of the chloroform extract from aerial parts of C. divaricatum, except for the mixtures of sesquiterpene lactones, which are known constituents of the plant [7], gave several fractions containing substantial amounts of the compound that showed a UV absorption maximum at 221 nm (HPLC-DAD). After multiple fractionation by means of chromatographic methods, the compound was isolated and identified, on the basis of its spectral data (see Section 4.5.), as trans-12-oxo-phytodienoic acid (12-OPDA, Figure 2).

2.3. Effect of trans-12-OPDA on Lipopolysaccharide (LPS)-Stimulated Release of Pro-Inflammatory Cytokines from Human Neutrophils

2.3.1. Cytotoxicity

Cytotoxicity of trans-12-OPDA to human polymorphonuclear leukocytes (PMNs) was assessed using propidium iodide (PI) staining and flow cytometry (FACS) analysis. The compound was essentially nontoxic in PMNs at 2.5 μM or lower concentrations (Figure 3). Solvent DMSO did not induce any cytotoxicity to the cells (data not shown). On the basis of these data, all further experiments were performed using trans-12-OPDA at concentrations up to 2.5 μM.

2.3.2. Reactive Oxygen Species (ROS) Production

Activation of PMNs at a site of inflammation induces an oxidative burst in these cells. The phenomenon is characterized by intense ROS production and liberation of proteolytic enzymes from azurophilic granules. An effect of trans-12-OPDA on ROS generation in PMNs was examined in response to cytochalasin A/N-formyl-Met-Leu-Phe (f-MLP) stimulation. The examined oxylipin efficiently reduced ROS release at 1–2.5 μM (Figure 4).

2.3.3. Release of Selected Proinflammatory Cytokines/Chemokines (IL-8, TNFα, IL-1β, CCL2)

Neutrophils, in response to stimulation with proinflammatory agonists, e.g., LPS or f-MLP, secrete an array of cytokines and chemokines, including TNFα, IL-1β, IL-8, and CCL2 [18,19]. Human neutrophils were pretreated with trans-12-OPDA before their priming with LPS. Using ELISA, we determined levels of IL-8, TNFα, IL-1β, and CCL2 in the culture medium 24 h after LPS stimulation. Preincubation of human neutrophils with trans-12-OPDA resulted in significant and dose-dependent inhibition of IL-8 production upon LPS stimulation (Figure 5A). The oxylipin was also active as an inhibitor of TNFα production. A statistically significant reduction in the release of this cytokine was achieved with all tested doses of the compound (see Figure 5B). Both IL-1β and CCL2 production by LPS-treated neutrophils were moderately and dose-dependently lowered by pretreatment with trans-12-OPDA at a dose of 0.5–2.5 μM (Figure 6A,B).

3. Discussion

Hydroxycinnamates, including chlorogenic acids, as common constituents of fruit, vegetables, and widely consumed beverages (coffee) are intrinsic components of the human diet with potential health-enhancing properties [20,21]. Some recent reports suggest their implication in prevention of so-called lifestyle diseases, such as diabetes [22,23], cardiovascular and neoplastic diseases [24,25], oxidative-stress-induced cognitive impairment [26,27,28,29], and obesity [30]. The Asteraceae plants are rich in hydroxycinnamates representing different structural types [21,31]. Plants of the Inuleae tribe are also good producers of acylquinic acids [31,32,33]. Despite their proven value as active plant metabolites, hydroxycinnamates have remained unexplored constituents of Carpesium sp. [3]. In the hydroalcoholic extract from aerial parts of C. divaricatum, we managed to detect 17 caffeic acid derivatives, using the HPLC-DAD-MSn method. Seven of them, including two monocaffeoylquinic acids, four dicaffeoylquinic acids (DCQAs), and 3,4,5-tri-O-caffeoylquinic acid, are quite common within the Asteraceae. Their chromatographic behavior and mass fragmentation patterns have been thoroughly studied before. Thus, their identification was not challenging. Conjugates of caffeic acid with hexaric acid (or acids) have been less investigated. Referring to our previous study, on caffeic acid derivatives from tissue culture of Inula helenium [14], we were able to identify three further compounds as dicaffeoylhexaric acid ([M − H]− 533) and two tricaffeoylhexaric acids ([M − H]− 695). The compounds were formerly reported as constituents of the Asteraceae plants belonging to the tribes Eupatorieae [11], Gnaphalieae [10,12], and Millerieae of the Heliantheae alliance [13,34]. While investigating phenolic compounds from flowerheads of Buphthalmum salicifolium L. (Asteraceae, Inuleae), Heilmann and coworkers [15] isolated 1-isobutyryl-3,5-DCQA. Its molecular ion—586 [M]+ and fragmentation pattern in negative ion mode nicely corresponded to those of the compound eluted at 19.8 min. The compound of the same molecular mass, eluted at 19.0 min, is very likely another isobutyryl ester of DCQA. Taking into consideration that esterification of both terpenoid and phenolic metabolites with isobutyric acid and/or 2-methylbutyric/isovaleric acid is quite common within Inuleae [7,35,36], we postulated that the compounds eluted at 22.9 min and 23.8 min were 2-methylbutyryl/isovaleryl-DCQAs. Finally, on the basis of spectral data of leontopodic acid, given by Schwaiger et al. [17], we tentatively identified the three remaining compounds as isobutyryl-tricaffeoylhexaric acid (m/z at 765 [M − H]−) and 2-methylbutyryl/isovaleryl-tricaffeoylhexaric acids (m/z at 779 [M − H]−). The estimated contents of 5-CQA (1.23% dry weight) and 3,5-DCQA (1.46% dry weight), together with the relation of peak areas of the two compounds to the areas of remaining hydroxycinnamate signals visible on the chromatogram, allow us to conclude that aerial parts of C. divaricatum could be a rich dietary source of these compounds [37].
12-OPDA is an intermediate in jasmonic acid biosynthesis in plants. The compound itself demonstrates physiological activity as a hormone regulating seed dormancy, embryo development, redox homeostasis, and response to stress [38]. As an optically active molecule, 12-OPDA may exist in four possible absolute configurations (9S,13S; 9S,13R; 9R,13R; and 9R,13S). On the basis of 1H-NMR spectral data, we were able to exclude cis orientation of side chains in our compound (i.e., 9S,13S and 9R,13R configurations) [39]. Cis or trans orientation of the side chains in 12-OPDA molecule can be easily determined by comparison of the chemical shift values for protons at C-8, C-9, and C-13 of the compound with those reported by Baertschi et al. [39]. Two protons at C-8 of the cis isomer give rise to two multiplets at δH 1.15 and 1.73 (1H each), whereas both protons at C-8 of the trans-12-OPDA give one signal at δH 1.49 (2H). Signals of protons at C-9 and C-13 of trans-12-OPDA are shifted upfield of those in the cis-OPDA by c. 0.4 ppm. Discrimination between the 9S,13R trans isomer and the 9R,13S trans isomer was possible by optical activity measurement. The dextrorotary activity of 12-OPDA isolated by us (see Section 4.5.) proved that the compound was (9S,13R)-12-OPDA, i.e., (+)-trans-12-OPDA [40]. Except for the activity as a plant hormone, 12-OPDA exerted cytoprotective effects in human neuroblastoma SH-SY5Y cells subjected to oxidative stress [41], mitigated LPS-induced inflammation in microglia [42], inhibited proliferation of human breast cancer cells [43], and demonstrated antileishmanial activity [44]. Thus, we decided to check whether or not the compound may contribute to the overall activity of C. divaricatum.
PMNs are an essential part of human immune system. They are, among other things, engaged in the inflammation process, which could be a consequence of bacterial infection, environmental stress, or some kinds of neoplastic disease. The neutrophils, except for performing phagocytosis, generating toxic molecules, releasing enzymes, and forming extracellular traps, participate in fine regulation of the immune response and inflammatory process via a capability to respond to and to produce a variety of cytokins [19]. Cytokins produced by human neutrophils include the proinflammatory cytokine IL-1β, TNFα, and the chemokines CCL2 and IL-8 (CXCL8). In order to establish whether or not trans-12-OPDA may influence neutrophil function, we decided to monitor secretion of the mentioned cytokines by LPS-stimulated human neutrophils in the absence or presence of the compound. LPS stimulation of neutrophils is mediated via toll-like receptors (TLRs), and this process includes activation of both the NF-κB and mitogen-activated protein (MAP) kinase pathways. At a site of inflammation, leucocytes are activated and, subsequently, an intense production of ROS by the cells takes place. We observed that incubation of human neutrophils with 12-OPDA significantly and in a concentration-dependent manner diminished f-MLP–induced ROS production by the cells (Figure 4). The examined oxylipin significantly reduced secretion of proinflammatory cytokines (IL-1β, TNFα) and chemokines CCL2 and IL-8 by LPS-stimulated human neutrophils (Figure 5 and Figure 6). The inhibitory effect exerted by the lowest concentration tested (0.5 μM) was slightly weaker towards IL-1β and CCL2 secretion (p < 0.05, Figure 6). The results of our tests suggested that 12-OPDA may be implicated in the anti-inflammatory activity of C. divaricatum preparations. Effects observed in vitro nicely corresponded to the traditional use of the plant.
In conclusion, we would like to emphasize that the aerial parts of C. divaricatum are a rich and still not completely explored source of bioactive molecules. The variety of their chemical structures and bioavailability after ingestion are far from being completely investigated. Though sesquiterpene lactones isolated from the plant have proven activity as cytotoxic and anti-inflammatory agents, they are not the sole compounds responsible for the pharmacological activity of the plant. The set of specialized metabolites produced by C. divaricatum suggests considerable potential for the future application of the plant either as a functional food or as an herbal medicinal product.

4. Materials and Methods

4.1. General Methods

Mass spectra were recorded using a MALDI-TOF/TOF ultrafleXtreme mass spectrometer (Bruker Daltonics, Billerica, MA, USA). 1H-NMR spectra were recorded in CDCl3 on a Bruker AVANCE III HD 400 (resonance frequency 400.17 MHz) spectrometer. Optical rotation was determined in CDCl3 on a PolAAr31 polarimeter (Optical Activity Ltd., Ramsey, England, UK). RP-HPLC separations were performed using an Agilent 1200 Series HPLC system (Agilent Technologies, Santa Clara, CA, USA) equipped with a diode array detector. Analytical chromatographic separations were carried out on a Kinetex XB-C18 column (4.6 × 250 mm, 5 μm total particle size; Phenomenex, Torrance, CA, USA). Semipreparative RP-HPLC was conducted on a Synergi 4μ Fusion-RP, 80A, 250 × 10 mm column (Phenomenex, Torrance, CA, USA), with an isocratic elution, using MeOH-H2O mixtures of different polarities. Conventional column chromatography was carried out on Silica gel 60 (0.063–0.2 mm, Merck, Darmstadt, Germany). TLC separations were performed using precoated plates (Silica gel 60 without fluorescence indicator, Art. No 5553, Merck, Germany).

4.2. Materials

Chlorogenic acid (5-O-CQA, purity > 97% by HPLC) and a standard sample of cynarin (1,3-DCQA, purity > 99% by HPLC) were purchased from Roth (Karlsruhe, Germany). Organic solvents of analytical grade were purchased either from POCh S.A. (Gliwice, Poland) or from Merck (Darmstadt, Germany). Water was purified by a Mili-Q system (Milipore Corp., Bedford, MA, USA). MeOH and MeCN of HPLC grade as well as formic acid and glacial acetic acid were purchased from Merck (Darmstadt, Germany).
Phosphate-buffered saline (PBS) was bought from Biomed (Lublin, Poland). Hanks’ balanced salt solution (HBSS), cell culture medium (RPMI 1640 medium), f-MLP (formyl-Met-Leu-Phenylalanine), LPS (from Escherichia coli 0111:B4), propidium iodide (PI), luminol, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) solution, and l-glutamine were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Fetal bovine serum (FBS) was delivered by Gibco (Grand Island, NY, USA).

4.3. Plant Material

C. divaricatum plants were grown in the Garden of Medicinal Plants, Institute of Pharmacology, Polish Academy of Sciences, Kraków from seeds that were provided by the Research Center for Medicinal Plant Resources, National Institute of Biomedical Innovation (Tsukuba, Japan). Aerial parts of the plants were collected in the beginning of flowering period (August/September) and dried under shade at room temperature. A voucher specimen (3/15) was deposited in the collection kept at the Garden of Medicinal Plants, Institute of Pharmacology, Kraków, Poland.

4.4. Analysis of Caffeic Acid Derivatives

4.4.1. Extract Preparation and Characterization by the HPLC-DAD-MSn Method

Dried and pulverized aerial parts of C. divaricatum (0.1 g) were extracted twice with 10 mL of 70% MeOH at room temperature, for 3 h, on a rotary shaker (100 r.p.m.). The extracts were combined and evaporated to dryness under reduced pressure to give a residue. The obtained residue (10 mg) was dissolved in a mixture of MeOH and 0.1% HCOOH (8:2, v/v) and then filtered through a 0.45 μm Chromafil membrane (Machery-Nagel, Duren, Germany). HPLC-DAD-MSn analysis was performed on a UHPLC-3000 RS system (Dionex, Germany) with a photodiode array (DAD) detection and an AmaZon SL ion trap mass spectrometer with an ESI interface (Bruker Daltonics GmbH, Germany). Separation was performed on a Kinetex XBC18 column (150 × 2.1 mm, 1.7 μm) Phenomenex (Torrance, CA, USA). The column temperature was 25 ºC. The mobile phase (A) was water/formic acid (100:0.1, v/v) and the mobile phase (B) was acetonitrile/formic acid (100:0.1, v/v). A gradient system was used: 0–10 min 10–25% B; 10–40 min 25–35% B. The flow rate was 0.3 mL/min. The column was equilibrated for 7 min between injections. UV spectra were recorded over a range of 200–450 nm, and chromatograms were acquired at 325 nm. The LC eluate was introduced directly into the ESI interface without splitting. The nebuliser pressure was 40 psi; dry gas flow 9 L/min; dry temperature 300 °C; and capillary voltage 4.5 kV. Analysis was carried out using a scan from m/z 90 to 2200. Compounds were analyzed in a negative ion mode. The MS2 fragmentation was obtained for the most abundant ion at the time.

4.4.2. Quantification of Chlorogenic Acid (5-CQA) and 3,5-di-O-caffeoylquinic Acid (3,5-DCQA)

The contents of 5-CQA and 3,5-DCQA, in the aerial parts of C. divaricatum, were estimated by means of the HPLC-DAD method, as was described earlier [14].

4.5. Isolation of Trans-12-Oxo-Phytodienoic Acid (12-OPDA)

Dry, pulverized shoots of C. divaricatum (267 g) were extracted four times with CHCl3 (1.5 L). The organic extracts were combined and concentrated in vacuo to provide 24 g of an oily residue. The residue was fractionated by conventional column chromatography (CC) on silica gel using an n-hexane–EtOAc gradient solvent system (up to 100% EtOAc). Collected fractions (50 mL each) were combined as shown by TLC. Fractions 175–181, eluted with n-hexane–EtOAc 7:3 (v/v), were subjected to semiprep. RP-HPLC (eluent: MeOH–H2O mixture, 4:1, v/v, isocratic mode, flow rate: 2 mL/min) to yield trans-12-OPDA (13.5 mg, purity > 92% by HPLC).

(+) trans-12-Oxo-phytodienoic acid: (9S,13R) OPDA

Amorphous solid: [α]D25.6: +69.3 (c = 0.5, CHCl3); UV (MeCN-H2O) λmax 221 nm; 1H-NMR (400.17 MHz, CDCl3): δ 7.62 (1H, dd, J = 5.7, 2.4 Hz, H-10), 6.15 (1H, dd, J = 5.7, 1.8 Hz, H-11), 5.47 (1H, m, H-16), 5.27 (1H, m, H-15), 2.59 (1H, m, H-9), 2.47 (1H, m, H-14a), 2.37 (2H, t, J = 7.4, H-2a, H-2b), 2.31 (1H, m, H-14b), 2.07 (2H, m, H-17a, H-17b), 2.04 (1H, m, H-13), 1.65 (2H, m, H-3a, H-3b), 1.50 (2H, m, H-8a, H-8b), 1.27–1.40 (8H, m, H-4a, H-4b, H-5a, H-5b, H-6a, H-6b, H-7a, H-7b), 0.98 (3H, t, J = 7.4). MALDI-MS/MS (pos. mode) m/z: 293.2 [C18H28O3 + H]+; calc. 293.2.

4.6. Isolation of Human Neutrophils

Peripheral venous blood was taken from healthy human donors (18–35 years old) in the Warsaw Blood Donation Centre. Donors did not smoke or take any medications. They were clinically recognized to be healthy and routine laboratory tests showed all values to be within the normal ranges. The study conformed to the principles of the Declaration of Helsinki. Neutrophils were isolated by dextran sedimentation and centrifugation in a Ficoll Hypaque gradient and then resuspended in (Ca2+)-free HBSS buffer or RPMI 1640 medium.

4.7. Assessment of the Effects of Trans-12-OPDA on Lipopolysaccharide (LPS)-Stimulated Release of Pro-Inflammatory Cytokines from Human Neutrophils

4.7.1. Cytotoxicity Measurement

Cytotoxicity was assessed by a standard flow cytometric probe using propidium iodide (PI) staining. After 24 h of incubation in the absence or presence trans-12-OPDA (at concentrations of 0.5, 1.0, 2.5, or 5.0 μM), the neutrophils (3.5 × 105) were harvested and centrifuged (1500 r.p.m.; 10 min; 4 °C), washed once with cold PBS, and resuspended in 400 μL of PBS. A 5 μL aliquot of PI solution (50 μg/mL) was added to the cell suspension. After 15 min of incubation with PI at room temperature, cells were analyzed by a BD FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA), and 10,000 events were recorded per sample. The number of cells that displayed high permeability to PI, expressed as a percentage of PI (+) cells, was determined.

4.7.2. ROS Production by Neutrophils

ROS production was measured using a luminol-dependent chemiluminescence test. A 70 μL aliquot of neutrophil suspension (3.5 × 105) in (Ca2+)-free HBSS buffer, 50 μL of the tested compound solution, and 50 μL of luminol (100 μM) were added to a well in a 96-well plate. ROS production was initiated by the addition of f-MLP (30 μL of 0.1 μg/mL solution) to obtain a total volume of 200 μL per well. Chemiluminescence changes were measured for 40 min, at 2 min intervals, in a microplate reader (37 °C). The background chemiluminescence produced by non-stimulated cells was also determined. The percentage of inhibition was calculated by comparison to the stimulated control without the tested compound, at the maximum luminescence.

4.7.3. IL-8, IL-1β, CCL-2, and TNFα Production by Neutrophils

Neutrophils (2 × 106) were cultured in 24-well plates in RPMI 1640 medium with 10% FBS, 10 mM HEPES, and 2 mM L-glutamine, in the presence or absence of LPS (100 ng/mL) and in the absence or presence of trans-12-OPDA (final concentration in a range of 0.5–5 μM), at 37 °C with 5% CO2. After 24 h, the neutrophils were harvested and centrifuged (2000 r.p.m.; 10 min; 4 °C). The amount of released cytokines was measured by enzyme-linked immunosorbent assay (ELISA) following the manufacturer’s instructions (BD Biosciences, USA). The effects on IL-8, IL-1β, CCL2, and TNFα production were calculated by comparing the percentages of the released agents to the stimulated control, which lacked the test compound.

4.8. Statistical Analysis

The results were expressed as the mean ± standard error of the mean (SEM) of three independent experiments performed at least in duplicate. All analyses were performed using Statistica 13 software. The statistical significance of the differences between means was established by ANOVA with Dunnett’s post hoc test p values.

Author Contributions

A.S.: concept of the work, analysis of spectroscopic data, and review of the manuscript; N.K. and J.M.: phytochemical work, isolation of compounds, and involvement in manuscript preparation; B.M., N.K., and A.K.K.: in vitro activity assays; A.K.K.: performance of HPLC-DAD-MSn analyses and involvement in manuscript preparation.

Funding

This research was funded by the Ministry of Science and Higher Education, Poland (statutory activity funding).

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Anderberg, A.A. Inuleae. In Systematics, Evolution, and Biogeography of Compositae; Funk, V.A., Susanna, A., Stuessy, T.F., Bayer, R.J., Eds.; International Association for Plant Taxonomy: Vienna, Austria, 2009; pp. 667–680. [Google Scholar]
  2. Hong, L.; Zhuo, J.; Lei, Q.; Zhou, J.; Ahmed, S.; Wang, C.; Long, Y.; Li, F.; Long, C. Ethnobotany of wild plants used for starting fermented beverages in Shui communities of southwest China. J. Ethnobiol. Ethnomed. 2015, 11, 42. [Google Scholar] [CrossRef] [PubMed]
  3. Zhang, J.-P.; Wang, G.-W.; Tian, X.-H.; Yang, Y.-X.; Liu, Q.-X.; Chen, L.-P.; Li, H.-L.; Zhang, W.-D. The genus Carpesium: A review of its ethnopharmacology, phytochemistry and pharmacology. J. Ethnopharmacol. 2015, 163, 173–191. [Google Scholar] [CrossRef]
  4. Kim, H.; Song, M.-J. Ethnobotanical analysis for traditional knowledge of wild edible plants in North Jeolla Province (Korea). Genet. Resour. Crop Evol. 2013, 60, 1571–1585. [Google Scholar] [CrossRef]
  5. Geng, Y.; Zhang, Y.; Ranjitkar, S.; Huai, H.; Wang, Y. Traditional knowledge and its transmission of wild edibles used by the Naxi in Baidi Village, northwest Yunnan province. J. Ethnobiol. Ethnomed. 2016, 12, 10. [Google Scholar] [CrossRef] [PubMed]
  6. Rivero, A.; Quintana, J.; Eiroa, J.L.; López, M.; Triana, J.; Bermejo, J.; Estévez, F. Potent induction of apoptosis by germacranolide sesquiterpene lactones on human myeloid leukemia cells. Eur. J. Pharmacol. 2003, 482, 77–84. [Google Scholar] [CrossRef] [PubMed]
  7. Zhang, T.; Chen, J.-H.; Si, J.-G.; Ding, G.; Zhang, Q.-B.; Zhang, H.-W.; Jia, H.-M.; Zou, Z.-M. Isolation, structure elucidation, and absolute configuration of germacrane isomers from Carpesium divaricatum. Sci. Rep. 2018, 8, 12418. [Google Scholar] [CrossRef]
  8. Clifford, M.N.; Knight, S.; Kuhnert, N. Discriminating between the six isomers of dicaffeoylquinic acid by LC-MSn. J. Agric. Food Chem. 2005, 53, 3821–3832. [Google Scholar] [CrossRef]
  9. Willems, J.L.; Khamis, M.M.; Saeid, W.M.; Purves, R.W.; Katselis, G.; Low, N.H.; El-Aneed, A. Analysis of a series of chlorogenic acid isomers using differential ion mobility and tandem mass spectrometry. Anal. Chim. Acta 2016, 933, 164–174. [Google Scholar] [CrossRef] [PubMed]
  10. Schwaiger, S.; Seger, C.; Wiesbauer, B.; Schneider, P.; Ellmerer, E.P.; Sturm, S.; Stuppner, H. Development of an HPLC-PAD-MS assay for the identification and quantification of major phenolic edelweiss (Leontopodium alpinum Cass.) constituents. Phytochem. Anal. 2006, 17, 291–298. [Google Scholar] [CrossRef] [PubMed]
  11. Maas, M.; Petereit, F.; Hensel, A. Caffeic acid derivatives from Eupatorium perfoliatum L. Molecules 2009, 14, 36–45. [Google Scholar] [CrossRef]
  12. Cicek, S.S.; Untersulzner, C.; Schwaiger, S.; Zidorn, C. Caffeoyl-D-glucaric acid derivatives in the genus Gnaphalium (Asteraceae: Gnaphalieae). Rec. Nat. Prod. 2012, 6, 311–315. [Google Scholar]
  13. Bazylko, A.; Boruc, K.; Borzym, J.; Kiss, A.K. Aqueous and ethanolic extracts of Galinsoga parviflora and Galinsoga ciliata. Investigations of caffeic acid derivatives and flavonoids by HPTLC and HPLC-DAD-MS methods. Phytochem. Lett. 2015, 11, 394–398. [Google Scholar] [CrossRef]
  14. Stojakowska, A.; Malarz, J.; Kiss, A.K. Hydroxycinnamates from elecampane (Inula helenium L.) callus culture. Acta Physiol. Plant. 2016, 38, 41. [Google Scholar] [CrossRef]
  15. Heilmann, J.; Müller, E.; Merfort, I. Flavonoid glucosides and dicaffeoylquinic acids from fowerheads of Buphthalmum salicifolium. Phytochemistry 1999, 51, 713–718. [Google Scholar] [CrossRef]
  16. Lin, L.-Z.; Harnly, J.M. Identification of hydroxycinnamoylquinic acids of arnica flowers and burdock roots using a standardized LC-DAD-ESI/MS profiling method. J. Agric. Food Chem. 2008, 56, 10105–10114. [Google Scholar] [CrossRef]
  17. Schwaiger, S.; Cervellati, R.; Seger, C.; Ellmerer, E.P.; About, N.; Renimel, I.; Godenir, C.; André, P.; Gafner, F.; Stuppner, H. Leontopodic acid—A novel highly substituted glucaric acid derivative from Edelweiss (Leontopodium alpinum Cass.) and its antioxidative and DNA protecting properties. Tetrahedron 2005, 61, 4621–4630. [Google Scholar] [CrossRef]
  18. McDonald, P.P.; Bald, A.; Cassatella, M. Activation of NF-κB pathway by inflammatory stimuli in human neutrophils. Blood 1997, 89, 3421–3433. [Google Scholar] [PubMed]
  19. Tecchio, C.; Micheletti, A.; Cassatella, M.A. Neutrophil-derived cytokines: Facts beyond expression. Front. Immunol. 2014, 5, 508. [Google Scholar] [CrossRef]
  20. Liang, N.; Kitts, D.D. Role of chlorogenic acids in controlling oxidative and inflammatory stress conditions. Nutrients 2016, 8, 16. [Google Scholar] [CrossRef]
  21. Clifford, M.N.; Jaganath, I.B.; Ludwig, I.A.; Crozier, A. Chlorogenic acids and the acyl-quinic acids: Discovery, biosynthesis, bioavailability and bioactivity. Nat. Prod. Rep. 2017, 34, 1391–1421. [Google Scholar] [CrossRef]
  22. Verzelloni, E.; Pellacani, C.; Tagliazucchi, D.; Tagliaferri, S.; Calani, L.; Costa, L.G.; Brighenti, F.; Borges, G.; Crozier, A.; Conte, A.; Del Rio, D. Antiglycative and neuroprotective activity of colon-derived polyphenol catabolites. Mol. Nutr. Food Res. 2011, 55, S35–S43. [Google Scholar] [CrossRef]
  23. Tousch, D.; Lajoix, A.-D.; Hosy, E.; Azay-Milhau, J.; Ferrare, K.; Jahannault, C.; Cros, G.; Petit, P. Chicoric acid, a new compound able to enhance insulin release and glucose uptake. Biochem. Biophys. Res. Commun. 2008, 377, 131–135. [Google Scholar] [CrossRef] [PubMed]
  24. Lu, Q.-B.; Wan, M.-Y.; Wang, P.-Y.; Zhang, C.-X.; Xu, D.-Y.; Liao, X.; Sun, H.-J. Chicoric acid prevents PDGF-BB-induced VSMC dedifferentiation, proliferation and migration by suppressing ROS/NFκB/mTOR/P70S6K signaling cascade. Redox Biol. 2018, 14, 656–668. [Google Scholar] [CrossRef]
  25. Tsai, Y.-L.; Chiu, C.-C.; Chen, J.Y.-F.; Chan, K.-C.; Lin, S.-D. Cytotoxic effects of Echinacea purpurea flower extracts and cichoric acid on human colon cancer cells through induction of apoptosis. J. Ethnopharm. 2012, 143, 914–919. [Google Scholar] [CrossRef] [PubMed]
  26. Kwon, S.-H.; Lee, H.-K.; Kim, J.-A.; Hong, S.-I.; Kim, H.-C.; Jo, T.-H.; Park, Y.-I.; Lee, C.-K.; Kim, Y.-B.; Lee, S.-Y.; et al. Neuroprotective effects of chlorogenic acid on scopolamine-induced amnesia via anti-acetylcholinesterase and anti-oxidative activities in mice. Eur. J. Pharmacol. 2010, 649, 210–217. [Google Scholar] [CrossRef] [PubMed]
  27. Han, J.; Miyamae, Y.; Shigemori, H.; Isoda, H. Neuroprotective effect of 3,5-di-O-caffeoylquinic acid on SH-SY5Y cells and senescence-accelerated-prone mice 8 through the up-regulation of phosphoglycerate kinase-1. Neuroscience 2010, 169, 1039–1045. [Google Scholar] [CrossRef] [PubMed]
  28. Liu, Q.; Hu, Y.; Cao, Y.; Song, G.; Liu, Z.; Liu, X. Chicoric acid ameliorates lipopolysaccharide-induced oxidative stress via promoting the Keap1/Nrf2 transcriptional signaling pathway in BV-2 microglial cells and mouse brain. J. Agric. Food Chem. 2017, 65, 338–347. [Google Scholar] [CrossRef]
  29. Wang, Y.; Diao, Z.; Li, J.; Ren, B.; Zhu, D.; Liu, Q.; Liu, Z.; Liu, X. Chicoric acid supplementation ameliorates cognitive impairment induced by oxidative stress via promotion of antioxidant defence system. RSC Adv. 2017, 7, 36149–36162. [Google Scholar] [CrossRef]
  30. Xiao, H.; Wang, J.; Yuan, L.; Xiao, C.; Wang, Y.; Liu, X. Chicoric acid induces apoptosis in 3T3-L1 preadipocytes through ROS-mediated PI3K/Akt and MAPK signaling pathways. J. Agric. Food Chem. 2013, 61, 1509–1520. [Google Scholar] [CrossRef]
  31. Jaiswal, R.; Kiprotich, J.; Kuhnert, N. Determination of the hydroxycinnamate profile of 12 members of the Asteraceae family. Phytochemistry 2011, 72, 781–790. [Google Scholar] [CrossRef]
  32. Zheleva-Dimitrova, D.; Gevrenova, R.; Zaharieva, M.M.; Najdenski, H.; Ruseva, S.; Lozanov, V.; Balabanova, V.; Yagi, S.; Momekov, G.; Mitev, V. HPLC-UV and LC-MS analyses of acylquinic acids in Geigeria alata (DC) Oliv. & Hiern. and their contribution to antioxidant and antimicrobial capacity. Phytochem. Anal. 2016, 28, 176–184. [Google Scholar] [PubMed]
  33. Boukhris, M.A.; Destandau, E.; El Hakmaoui, A.; El Rhaffari, L.; Elfakir, C. A dereplication strategy for the identification of new phenolic compounds from Anvillea radiata (Coss. & Durieu). C.R. Chim. 2016, 19, 1124–1132. [Google Scholar]
  34. Takenaka, M.; Yan, X.; Ono, H.; Yoshida, M.; Nagata, T.; Nakanishi, T. Caffeic acid derivatives in the roots of yacon (Smallanthus sonchifolius). J. Nat. Prod. 2003, 51, 793–796. [Google Scholar] [CrossRef] [PubMed]
  35. Park, E.J.; Kim, Y.; Kim, J. Acylated flavonol glycosides from the flower of Inula britannica. J. Nat. Prod. 2000, 63, 34–36. [Google Scholar] [CrossRef] [PubMed]
  36. Stojakowska, A.; Malarz, J.; Żylewski, M.; Kisiel, W. Acylated hydroxycinnamic acid glucosides from flowers of Telekia speciosa. Phytochem. Lett. 2015, 12, 257–261. [Google Scholar] [CrossRef]
  37. Stojakowska, A.; Michalska, K.; Kłeczek, N.; Malarz, J.; Beharav, A. Phenolics and terpenoids from a wild edible plant Lactuca orientalis (Boiss.) Boiss.: A preliminary study. J. Food Comp. Anal. 2018, 69, 20–24. [Google Scholar] [CrossRef]
  38. Maynard, D.; Gröger, H.; Dierks, T.; Dietz, K.-J. The function of the oxylipin 12-oxophytodienoic acid in cell signaling, stress acclimation, and development. J. Exp. Bot. 2018, 69, 5341–5354. [Google Scholar] [CrossRef]
  39. Baertschi, S.W.; Ingram, C.D.; Harris, T.M.; Brash, A.R. Absolute configuration of cis-12-oxophytodienoic acid of flaxseed: Implications for the mechanism of biosynthesis from the 13(S)-hydroperoxide of linolenic acid. Biochemistry 1988, 27, 18–24. [Google Scholar] [CrossRef]
  40. Schaller, F.; Hennig, P.; Weiler, E.W. 12-Oxophytodienoate-10,11-reductase: Occurrence of two isoenzymes of different specificity against stereoisomers of 12-oxophytodienoic acid. Plant Physiol. 1998, 118, 1345–1351. [Google Scholar] [CrossRef]
  41. Taki-Nakano, N.; Ohzeki, H.; Kotera, J.; Ohta, H. Cytoprotective effects of 12-oxo phytodienoic acid, a plant-derived oxylipin jasmonate, on oxidative stress-induced toxicity in human neuroblastoma SH-SY5Y cells. Biochim. Biophys. Acta 2014, 1840, 3413–3422. [Google Scholar] [CrossRef]
  42. Taki-Nakano, N.; Kotera, J.; Ohta, H. 12-oxo-phytodienoic acid, a plant-derived oxylipin, attenuates lipopolysaccharide-induced inflammation in microglia. Biochem. Biophys. Res. Commun. 2016, 473, 1288–1294. [Google Scholar] [CrossRef]
  43. Altiok, N.; Mezzadra, H.; Patel, P.; Koyuturk, M.; Altiok, S. A plant oxylipin, 12-oxo-phytodienoic acid, inhibits proliferation of human breast cancer cells by targeting cyclin D1. Breast Cancer Res. Treat. 2008, 109, 315–323. [Google Scholar] [CrossRef]
  44. Maldonado, E.M.; Salamanca, E.; Giménez, A.; Sterner, O. Antileishmanial metabolites from Lantana balansae. Rev. Bras. Farmacogn. 2016, 26, 180–183. [Google Scholar] [CrossRef]
Sample Availability: Samples of the compounds are not available from the authors.
Molecules 24 01614 g001
Figure 1. An HPLC-UV chromatogram of Carpesium divaricatum aerial part extract (10 mg/mL, 2 μL injected) acquired at 325 nm.
Figure 1. An HPLC-UV chromatogram of Carpesium divaricatum aerial part extract (10 mg/mL, 2 μL injected) acquired at 325 nm.
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Molecules 24 01614 g002
Figure 2. The chemical structure of (+)-trans-12-oxo-phytodienoic acid (trans-12-OPDA) isolated from aerial parts of Carpesium divaricatum Sieb. & Zucc.
Figure 2. The chemical structure of (+)-trans-12-oxo-phytodienoic acid (trans-12-OPDA) isolated from aerial parts of Carpesium divaricatum Sieb. & Zucc.
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Molecules 24 01614 g003
Figure 3. Cytotoxic effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on lipopolysaccharide (LPS)-stimulated human neutrophils. Results are shown as percent of cells with diminished membrane integrity (propidium-iodide-positive cells, PI (+)). Control, untreated cells; LPS, cells stimulated with LPS (stimulated control). Statistical significance * p < 0.05, with reference to a stimulated control.
Figure 3. Cytotoxic effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on lipopolysaccharide (LPS)-stimulated human neutrophils. Results are shown as percent of cells with diminished membrane integrity (propidium-iodide-positive cells, PI (+)). Control, untreated cells; LPS, cells stimulated with LPS (stimulated control). Statistical significance * p < 0.05, with reference to a stimulated control.
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Molecules 24 01614 g004
Figure 4. Inhibitory effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on reactive oxygen species (ROS) release from f-MLP-stimulated human neutrophils. Statistical significance *** p < 0.001, with reference to a stimulated control.
Figure 4. Inhibitory effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on reactive oxygen species (ROS) release from f-MLP-stimulated human neutrophils. Statistical significance *** p < 0.001, with reference to a stimulated control.
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Molecules 24 01614 g005
Figure 5. Inhibitory effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on IL-8 (A) and TNFα (B) secretion by LPS-stimulated human neutrophils. Statistical significance *** p < 0.001, with reference to a stimulated control.
Figure 5. Inhibitory effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on IL-8 (A) and TNFα (B) secretion by LPS-stimulated human neutrophils. Statistical significance *** p < 0.001, with reference to a stimulated control.
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Molecules 24 01614 g006
Figure 6. Inhibitory effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on IL-1β (A) and CCL2 (B) secretion by LPS-stimulated human neutrophils. Statistical significance: * p < 0.05, *** p < 0.001, with reference to a stimulated control.
Figure 6. Inhibitory effects of trans-12-OPDA, at concentrations of 0.5, 1.0, and 2.5 μM, on IL-1β (A) and CCL2 (B) secretion by LPS-stimulated human neutrophils. Statistical significance: * p < 0.05, *** p < 0.001, with reference to a stimulated control.
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Table 1. Retention times, UV maxima, and MSn data, in the negative ion mode, for the phenolic compounds present in extracts from Carpesium divaricatum aerial parts.
Table 1. Retention times, UV maxima, and MSn data, in the negative ion mode, for the phenolic compounds present in extracts from Carpesium divaricatum aerial parts.
CompoundRetention Time (min)UV (nm)[M − H]− Product Ions Main Peaks 1
13-O-caffeoylquinic acid3.2325353191, 179
25-O-caffeoylquinic acid5.1325353191
3Dicaffeoylhexaric acid (I)7.9324533371, 209
43,4-di-O-caffeoylquinic acid12.6325515353, 335, 299, 255, 203, 191, 179, 173
51,5-di-O-caffeolyquinic acid12.8328515353, 335, 191
63,5-di-O-caffeoylquinic acid13.0327515353, 191
7tricaffeoylhexaric acid (I)13.5327695533, 371, 209
84,5-di-O-caffeoylquinic acid13.8327515353, 317, 299, 255, 203, 173
9tricaffeoylhexaric acid (II)14.8328695533, 371, 209
10isobutyryl-dicaffeoylquinic acid19.0326585497, 335, 317, 299, 255, 179
11isobutyryl-dicaffeoylquinic acid19.8328585497, 423, 335, 179
12tri-O-caffeoylquinic acid20.1327677515, 353
132-methylbutyryl/isovaleryl-dicaffeoylquinic acid22.9326599497, 335, 299, 179
142-methylbutyryl/isovaleryl-dicaffeoylquinic acid23.8328599497, 437, 335, 179
15isobutyryl-tricaffeoylhexaric acid24.7328765603, 441
162-methylbutyryl/isovaleryl-tricaffeoylhexaric acid29.7327779617, 455
172-methylbutyryl/isovaleryl-tricaffeoylhexaric acid30.2327779617, 455
1 MS2 ions in bold are the most abundant ion peak.

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Kłeczek, N.; Michalak, B.; Malarz, J.; Kiss, A.K.; Stojakowska, A. Carpesium divaricatum Sieb. & Zucc. Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant. Molecules 2019, 24, 1614. https://doi.org/10.3390/molecules24081614

AMA Style

Kłeczek N, Michalak B, Malarz J, Kiss AK, Stojakowska A. Carpesium divaricatum Sieb. & Zucc. Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant. Molecules. 2019; 24(8):1614. https://doi.org/10.3390/molecules24081614

Chicago/Turabian Style

Kłeczek, Natalia, Barbara Michalak, Janusz Malarz, Anna Karolina Kiss, and Anna Stojakowska. 2019. "Carpesium divaricatum Sieb. & Zucc. Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant" Molecules 24, no. 8: 1614. https://doi.org/10.3390/molecules24081614

APA Style

Kłeczek, N., Michalak, B., Malarz, J., Kiss, A. K., & Stojakowska, A. (2019). Carpesium divaricatum Sieb. & Zucc. Revisited: Newly Identified Constituents from Aerial Parts of the Plant and Their Possible Contribution to the Biological Activity of the Plant. Molecules, 24(8), 1614. https://doi.org/10.3390/molecules24081614

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