Design, Synthesis, and Evaluation of Monoamine Oxidase A Inhibitors–Indocyanine Dyes Conjugates as Targeted Antitumor Agents
AbstractMonoamine oxidase A (MAOA) is an important mitochondria-bound enzyme that catalyzes the oxidative deamination of monoamine neurotransmitters. Accumulating evidence suggests a significant association of increased MAOA expression and advanced high-grade prostate cancer (PCa) progression and metastasis. Herein, a series of novel conjugates combining the MAOA inhibitor isoniazid (INH) and tumor-targeting near-infrared (NIR) heptamethine cyanine dyes were designed and synthesized. The synthesized compounds G1–G13 were evaluated in vitro for their cytotoxicity against PC-3 cells using the MTT assay, and molecular docking studies were performed. Results showed that most tested compounds exhibited improved antitumor efficacy compared with INH. Moreover, conjugates G10 and G11 showed potent anticancer activity with IC50 values (0.85 and 0.4 μM respectively) comparable to that of doxorubicin (DOX). This may be attributable to the preferential accumulation of these conjugates in tumor cells. G10, G11, and G12 also demonstrated moderate MAOA inhibitory activities. This result and the results of molecular docking studies were consistent with their cytotoxicity activities. Taken together, these data suggest that a combination of the MAOA inhibitor INH with tumor-targeting heptamethine cyanine dyes may prove to be a highly promising tool for the treatment of advanced prostate cancer. View Full-Text
- Supplementary File 1:
PDF-Document (PDF, 2342 KB)
Share & Cite This Article
Yang, X.-G.; Mou, Y.-H.; Wang, Y.-J.; Wang, J.; Li, Y.-Y.; Kong, R.-H.; Ding, M.; Wang, D.; Guo, C. Design, Synthesis, and Evaluation of Monoamine Oxidase A Inhibitors–Indocyanine Dyes Conjugates as Targeted Antitumor Agents. Molecules 2019, 24, 1400.
Yang X-G, Mou Y-H, Wang Y-J, Wang J, Li Y-Y, Kong R-H, Ding M, Wang D, Guo C. Design, Synthesis, and Evaluation of Monoamine Oxidase A Inhibitors–Indocyanine Dyes Conjugates as Targeted Antitumor Agents. Molecules. 2019; 24(7):1400.Chicago/Turabian Style
Yang, Xiao-Guang; Mou, Yan-Hua; Wang, Yong-Jun; Wang, Jian; Li, Yan-Yu; Kong, Rui-Heng; Ding, Meng; Wang, Dun; Guo, Chun. 2019. "Design, Synthesis, and Evaluation of Monoamine Oxidase A Inhibitors–Indocyanine Dyes Conjugates as Targeted Antitumor Agents." Molecules 24, no. 7: 1400.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.