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Enhanced Anti-Allergic Activity of Milk Casein Phosphopeptide by Additional Phosphorylation in Ovalbumin-Sensitized Mice

1
Interdisciplinary Graduate School of Science and Technology, Shinshu University, 8304 Minamiminowa, Ina, Nagano 399-4598, Japan
2
Department of Agricultural Research, Animal Production and Range research Division, Ministry of Agriculture, Private bag 0033, Gaborone BO320, Botswana
3
Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, Ina, Nagano 399-4598, Japan
*
Author to whom correspondence should be addressed.
Molecules 2019, 24(4), 738; https://doi.org/10.3390/molecules24040738
Received: 9 January 2019 / Revised: 11 February 2019 / Accepted: 17 February 2019 / Published: 19 February 2019
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Abstract

The proteolytic digest of milk casein, known as casein phosphopeptide (CPP-III), exhibits diverse biological activities, including calcium absorption and antioxidant activities. We hypothesized that the additional phosphorylation of this peptide can enhance its immunomodulatory activity such as suppression of allergy-associated cytokine and antigen-specific immune response. This study was conducted to assess whether oral intake of additionally phosphorylated CPP-III (P-CPP) attenuates ovalbumin (OVA)-induced IgE-mediated allergic reactions because of the additional phosphate groups. Female BALB/c mice were intraperitoneally sensitized with OVA twice at intervals of 14 days and then orally fed native CPP-III (N-CPP), P-CPP, and dephosphorylated CPP-III (D-CPP) for 6 weeks. Next, the mice were orally challenged with 50 mg of OVA. Oral administration of P-CPP suppressed total and specific IgE levels in the serum. Mice fed P-CPP exhibited low levels of OVA-specific IgG1 and increased OVA-specific IgG2a. P-CPP also suppressed IL-4 production, while D-CPP showed similar a level compared to that of the control. Further, P-CPP increased the population of the T follicular helper (Tfh) cell in the spleen. These results suggest that additional phosphorylation of CPP can enhance the attenuation of allergen-specific IgE-modulated allergic reactions in a murine food allergy model. View Full-Text
Keywords: phosphopeptide; phosphorylation; anti-allergic activity; immunomodulation phosphopeptide; phosphorylation; anti-allergic activity; immunomodulation
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Lebetwa, N.; Suzuki, Y.; Tanaka, S.; Nakamura, S.; Katayama, S. Enhanced Anti-Allergic Activity of Milk Casein Phosphopeptide by Additional Phosphorylation in Ovalbumin-Sensitized Mice. Molecules 2019, 24, 738.

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