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Open AccessArticle

Study of Interactions between Amlodipine and Quercetin on Human Serum Albumin: Spectroscopic and Modeling Approaches

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Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, 83232 Bratislava, Slovakia
2
Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, 83232 Bratislava, Slovakia
3
Department of Nanobiotechnology, University of Natural Resources and Life Sciences, 1190 Vienna, Austria
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Department of Chemistry, University of Natural Resources and Life Sciences, 1190 Vienna, Austria
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Department of Chemical Theory of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, 83232 Bratislava, Slovakia
*
Author to whom correspondence should be addressed.
Academic Editors: Marian Brestic, Marek Zivcak, Oksana Sytar and Marco Landi
Molecules 2019, 24(3), 487; https://doi.org/10.3390/molecules24030487
Received: 14 December 2018 / Revised: 26 January 2019 / Accepted: 29 January 2019 / Published: 30 January 2019
The aim of this study was to analyze the binding interactions between a common antihypertensive drug (amlodipine besylate—AML) and the widely distributed plant flavonoid quercetin (Q), in the presence of human serum albumin (HSA). Fluorescence analysis was implemented to investigate the effect of ligands on albumin intrinsic fluorescence and to define the binding and quenching properties. Further methods, such as circular dichroism and FT-IR, were used to obtain more details. The data show that both of these compounds bind to Sudlow’s Site 1 on HSA and that there exists a competitive interaction between them. Q is able to displace AML from its binding site and the presence of AML makes it easier for Q to bind. AML binds with the lower affinity and if the binding site is already occupied by Q, it binds to the secondary binding site inside the same hydrophobic pocket of Sudlow’s Site 1, with exactly the same affinity. Experimental data were complemented with molecular docking studies. The obtained results provide useful information about possible pharmacokinetic interactions upon simultaneous co-administration of the food/dietary supplement and the antihypertensive drug. View Full-Text
Keywords: human serum albumin; amlodipine; quercetin; fluorescence; circular dichroism; FT-IR; molecular modeling human serum albumin; amlodipine; quercetin; fluorescence; circular dichroism; FT-IR; molecular modeling
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Vaneková, Z.; Hubčík, L.; Toca-Herrera, J.L.; Furtműller, P.G.; Valentová, J.; Mučaji, P.; Nagy, M. Study of Interactions between Amlodipine and Quercetin on Human Serum Albumin: Spectroscopic and Modeling Approaches. Molecules 2019, 24, 487.

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