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Open AccessArticle

Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator

College of Pharmacy, Qiqihar Medical University, Qiqihar 161006, China
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Molecules 2019, 24(18), 3328; https://doi.org/10.3390/molecules24183328
Received: 12 August 2019 / Revised: 7 September 2019 / Accepted: 10 September 2019 / Published: 12 September 2019
(This article belongs to the Section Chemical Biology)
A new cyan fluorescent probe, MIPY-DNBS, using an imidazo[1,5-α]pyridine derivative as the fluorophore and 2,4-dinitrobenzensufonate as the recognition site for the selective detection of thiols (Cys, GSH, and Hcy), was designed and synthesized. Probe MIPY-DNBS exhibited a 172 nm Stokes shift, a fast response time (400 s), low cytotoxicity, low detection limits (12.7 nM for Cys), and excellent selectively in the detection of thiols. In addition, MIPY-DNBS was successfully applied to imaging thiols in living MCF-7 cells and zebrafish. View Full-Text
Keywords: large Stokes shift; zebrafish; thiols; cell imaging; imidazo[1,5-α]pyridine large Stokes shift; zebrafish; thiols; cell imaging; imidazo[1,5-α]pyridine
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MDPI and ACS Style

Chen, S.; Hou, P.; Sun, J.; Wang, H.; Liu, L. Recognition of Thiols in Living Cells and Zebrafish Using an Imidazo[1,5-α]pyridine-Derivative Indicator. Molecules 2019, 24, 3328.

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