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Open AccessArticle

Re-Potentiation of β-Lactam Antibiotic by Synergistic Combination with Biogenic Copper Oxide Nanocubes against Biofilm Forming Multidrug-Resistant Bacteria

Mepco Schlenk Engineering College, Sivakasi 626005, Tamil Nadu, India
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Molecules 2019, 24(17), 3055; https://doi.org/10.3390/molecules24173055
Received: 28 July 2019 / Revised: 18 August 2019 / Accepted: 20 August 2019 / Published: 22 August 2019
(This article belongs to the Section Nanochemistry)
Biofilm-associated tissue and device infection is a major threat to therapy. The present work aims to potentiate β-lactam antibiotics with biologically synthesized copper oxide nanoparticles. The synergistic combination of amoxyclav with copper oxide nanoparticles was investigated by checkerboard assay and time-kill assay against bacteria isolated from a burn wound and a urinary catheter. The control of biofilm formation and extracellular polymeric substance production by the synergistic combination was quantified in well plate assay. The effect of copper oxide nanoparticles on the viability of human dermal fibroblasts was evaluated. The minimum inhibitory concentration and minimum bactericidal concentration of amoxyclav were 70 μg/mL and 140 μg/mL, respectively, against Proteus mirabilis and 50 μg/mL and 100 μg/mL, respectively, against Staphylococcus aureus. The synergistic combination of amoxyclav with copper oxide nanoparticles reduced the minimum inhibitory concentration of amoxyclav by 16-fold against P. mirabilis and 32-fold against S. aureus. Above 17.5 μg/mL, amoxyclav exhibited additive activity with copper oxide nanoparticles against P. mirabilis. The time-kill assay showed the efficacy of the synergistic combination on the complete inhibition of P. mirabilis and S. aureus within 20 h and 24 h, respectively, whereas amoxyclav and copper oxide nanoparticles did not inhibit P. mirabilis and S. aureus until 48 h. The synergistic combination of amoxyclav with copper oxide nanoparticles significantly reduced the biofilm formed by P. mirabilis and S. aureus by 85% and 93%, respectively. The concentration of proteins, carbohydrates, and DNA in extracellular polymeric substances of the biofilm was significantly reduced by the synergistic combination of amoxyclav and copper oxide nanoparticles. The fibroblast cells cultured in the presence of copper oxide nanoparticles showed normal morphology with 99.47% viability. No cytopathic effect was observed. Thus, the study demonstrated the re-potentiation of amoxyclav by copper oxide nanoparticles. View Full-Text
Keywords: amoxyclav; burn wound; fibroblasts; Tamarindus indica; urinary catheter amoxyclav; burn wound; fibroblasts; Tamarindus indica; urinary catheter
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MDPI and ACS Style

Arul Selvaraj, R.C.; Rajendran, M.; Nagaiah, H.P. Re-Potentiation of β-Lactam Antibiotic by Synergistic Combination with Biogenic Copper Oxide Nanocubes against Biofilm Forming Multidrug-Resistant Bacteria. Molecules 2019, 24, 3055. https://doi.org/10.3390/molecules24173055

AMA Style

Arul Selvaraj RC, Rajendran M, Nagaiah HP. Re-Potentiation of β-Lactam Antibiotic by Synergistic Combination with Biogenic Copper Oxide Nanocubes against Biofilm Forming Multidrug-Resistant Bacteria. Molecules. 2019; 24(17):3055. https://doi.org/10.3390/molecules24173055

Chicago/Turabian Style

Arul Selvaraj, Ruby C.; Rajendran, Mala; Nagaiah, Hari P. 2019. "Re-Potentiation of β-Lactam Antibiotic by Synergistic Combination with Biogenic Copper Oxide Nanocubes against Biofilm Forming Multidrug-Resistant Bacteria" Molecules 24, no. 17: 3055. https://doi.org/10.3390/molecules24173055

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