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Transfer of a Multiclass Method for over 60 Antibiotics in Food from High Resolution to Low Resolution Mass Spectrometry

1
Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati”, 06126 Perugia, Italy
2
Department of Pharmaceutical Sciences, University of Perugia, 06123 Perugia, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Alessandra Gentili and Chiara Fanali
Molecules 2019, 24(16), 2935; https://doi.org/10.3390/molecules24162935
Received: 21 July 2019 / Revised: 8 August 2019 / Accepted: 10 August 2019 / Published: 13 August 2019
(This article belongs to the Special Issue Advances in Food Analysis)
PDF [622 KB, uploaded 13 August 2019]
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Abstract

A multiclass method has been developed to screen and confirm a wide range of anti-microbial residues in muscle and milk, and validated using liquid-chromatography coupled to (low-resolution, LR) tandem mass spectrometry (LC-QqQ). Over sixty antibiotics, belonging to ten distinct families, were included in the method scope. The development process was rapidly concluded as a result of two previously implemented methods. This consisted of identical sample treatments, followed by liquid chromatography, and coupled with high-resolution (HR) mass spectrometry (LC-Q-Orbitrap). The validation study was performed in the range between 10–1500 μg·kg−1 for muscles and 2–333 μg·kg−1 for milk. The main performance characteristics were estimated and, then, compared to those previously obtained with HR technique. The validity of the method transfer was ascertained also through inter-laboratory studies.
Keywords: antibiotics; liquid chromatography mass spectrometry; milk; muscle; validation antibiotics; liquid chromatography mass spectrometry; milk; muscle; validation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Giusepponi, D.; Paoletti, F.; Barola, C.; Moretti, S.; Saluti, G.; Ianni, F.; Sardella, R.; Galarini, R. Transfer of a Multiclass Method for over 60 Antibiotics in Food from High Resolution to Low Resolution Mass Spectrometry. Molecules 2019, 24, 2935.

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