Next Article in Journal
On the Effect of Aqueous Ammonia Soaking Pre-Treatment on Continuous Anaerobic Digestion of Digested Swine Manure Fibers
Previous Article in Journal
Cellulose Nanocrystal Surface Cationization: A New Fungicide with High Activity against Phycomycetes capsici
Previous Article in Special Issue
Rapid Determination of Amino Acids of Nitraria tangutorum Bobr. from the Qinghai-Tibet Plateau Using HPLC-FLD-MS/MS and a Highly Selective and Sensitive Pre-Column Derivatization Method
Article Menu
Issue 13 (July-1) cover image

Export Article

Open AccessArticle

From Fish Eggs to Fish Name: Caviar Species Discrimination by COIBar-RFLP, an Efficient Molecular Approach to Detect Fraud in the Caviar Trade

Department of Biological, Geological and Environmental Sciences–Section of Animal Biology “M. La Greca”, University of Catania, Via Androne 81, 95124 Catania, Italy
Department of Biology, University of Naples “Federico II”, Via Cinthia Ed7, 80126 Naples, Italy
Author to whom correspondence should be addressed.
Academic Editor: Daniel Cozzolino
Molecules 2019, 24(13), 2468;
Received: 15 May 2019 / Revised: 2 July 2019 / Accepted: 3 July 2019 / Published: 5 July 2019
(This article belongs to the Special Issue Food Analytical Chemistry–Advance Instrumental Methods and Sensors)
PDF [1191 KB, uploaded 5 July 2019]


The demand for caviar is growing as is its price on the market. Due to the decline of true caviar production from sturgeons, eggs from other fish species and other animals have been used as substitutes for caviar. The labels on these products should indicate the species from which the eggs were derived, but the label can be misleading in some cases. In this context, species identification using DNA analysis is crucial for traceability and authentication of caviar products. In this work, we applied the COIBar-RFLP procedure to obtain species-specific endonuclease restriction patterns useful to discriminate “caviar” species. The tested caviar products were identified as originating from eight species: Acipenser transmontanus, A. gueldenstaedtii, A. stellatus, A. baerii, Mallotus villosus, Huso huso, Cyclopterus lumpus and Eumicrotremus orbis. The results demonstrated that 14% of the caviar products examined have a label that does not indicate the species from which the eggs were originated. The MboI restriction enzyme produced specific profiles discriminating the eight species, confirming that the COIBar-RFLP is a useful approach for routine screening of seafood products due to its ease and rapid execution, as the results of screening can be obtained within 7 h, by-passing the need for sequencing. View Full-Text
Keywords: caviar; species identification; DNA barcoding; COIBar-RFLP caviar; species identification; DNA barcoding; COIBar-RFLP

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Pappalardo, A.M.; Petraccioli, A.; Capriglione, T.; Ferrito, V. From Fish Eggs to Fish Name: Caviar Species Discrimination by COIBar-RFLP, an Efficient Molecular Approach to Detect Fraud in the Caviar Trade. Molecules 2019, 24, 2468.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top