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Development and Evaluation of Copper Electrodes, Modified with Bimetallic Nanoparticles, to be Used as Sensors of Cysteine-Rich Peptides Synthesized by Tobacco Cells Exposed to Cytotoxic Levels of Cadmium

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Escuela de Ciencias Químicas, Pontificia Universidad Católica del Ecuador, Avenida 12 de Octubre y Roca, P.O.Box 17-01-2184, Quito, Ecuador
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Facultad de Ingeniería Química y Agroindustria, Escuela Politécnica Nacional, Ladrón de Guevara E11-253, Quito, Ecuador
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Departamento de Química, Universidad Simón Bolívar, Apartado 89000, Caracas, Venezuela
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Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas ESPE, Laboratorio de Cultivo de Tejidos Vegetales, Grupo BIOCEMP, Av. General Rumiñahui s/n, Sangolqui, P.O.Box 171-5-231B, Ecuador
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Hidroecuador, Apartado EC1701, Quito, Ecuador
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Authors to whom correspondence should be addressed.
Molecules 2019, 24(12), 2200; https://doi.org/10.3390/molecules24122200
Received: 24 February 2019 / Revised: 13 April 2019 / Accepted: 15 April 2019 / Published: 12 June 2019
We report on two new electrochemical sensors which, coupled to differential pulse voltammetry, constitutes a useful tool for diagnosis of heavy metal pollution. The electrochemical sensors AgHgNf/Cu and the AgBiNf/Cu were obtained by deposition of bimetallic particles of AgHg or AgBi on copper electrodes covered with a Nafion (Nf) film, respectively. Micrographs of the electrode’s surface showed evenly scattered bimetallic particles, with an approximate diameter of 150 nm, embedded in the Nafion (Nf) film. In order to test the electrodes, the hydrogen evolution signal according to the Brdička reaction was measured for the determination of cysteine-rich peptides (CRp) produced by plants. To check the accuracy of the electrodes, real samples of Nicotiana tabacum cells exposed to cytotoxic levels of cadmium were tested. The AgHgNf/Cu electrode produced detection limits (DLs) of 0.088 µmol L−1 for Cysteine and 0.139µmol L−1 for Glutathione, while for the AgBiNf/Cu electrode DLs were 0.41 µmol L−1 for cysteine and 0.244 µmol L−1 for glutathione. Thus, the new electrodes could be a useful analytical electrochemical system very convenient for fieldwork. The electrodes were capable of direct, accurate, and sensitive detection of synthesized peptides, despite the complex matrix where the Nicotiana tabacum cells were grown. View Full-Text
Keywords: Brdička reaction; electrochemical detection; cysteine-rich peptides; bimetallic deposits; heavy metal pollution Brdička reaction; electrochemical detection; cysteine-rich peptides; bimetallic deposits; heavy metal pollution
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Velasco-Medina, C.; Espinoza-Montero, P.J.; Montero-Jimenez, M.; Alvarado, J.; Jadán, M.; Carrera, P.; Fernandez, L. Development and Evaluation of Copper Electrodes, Modified with Bimetallic Nanoparticles, to be Used as Sensors of Cysteine-Rich Peptides Synthesized by Tobacco Cells Exposed to Cytotoxic Levels of Cadmium. Molecules 2019, 24, 2200.

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