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Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression

1
Department of Pharmacology, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok 10330, Thailand
2
Program in Veterinary Biosciences, Faculty of Veterinary Sciences, Chulalongkorn University, Bangkok 10330, Thailand
3
Department of Biological sciences, Florida International University, Miami, FL 33199, USA
4
Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Naresuan University, Ta-po, Phitsanulok 65000, Thailand
5
Department of Home Economics, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
6
Department of Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand
*
Author to whom correspondence should be addressed.
Academic Editors: Francesca Giampieri and Diego Muñoz-Torrer
Molecules 2019, 24(10), 1894; https://doi.org/10.3390/molecules24101894
Received: 13 March 2019 / Revised: 28 April 2019 / Accepted: 14 May 2019 / Published: 17 May 2019
(This article belongs to the Special Issue Nutraceuticals and Their Medicinal Importance 2019)
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Abstract

Clitoria ternatea (commonly known as blue pea) flower petal extract (CTE) is used as a natural colorant in a variety of foods and beverages. The objective of study was to determine the inhibitory effect of CTE on adipogenesis in 3T3-L1 preadipocytes. The phytochemical profiles of CTE were analyzed by liquid chromatography and tandem mass spectrometry (LC-MS/MS). Anti-adipogenesis effect of CTE was measured by using Oil Red O staining, intracellular triglyceride assay, quantitative real-time PCR and western blot analysis in 3T3-L1 adipocytes. Cell cycle studies were performed by flow cytometry. Lipolysis experiments were performed using a colorimetric assay kit. In early stages, CTE demonstrated anti-adipogenic effects through inhibition of proliferation and cell cycle retardation by suppressing expression of phospho-Akt and phospho-ERK1/2 signaling pathway. The results also showed that CTE inhibited the late stage of differentiation through diminishing expression of adipogenic transcription factors including PPARγ and C/EBPα. The inhibitory action was subsequently attenuated in downregulation of fatty acid synthase and acetyl-CoA carboxylase, causing the reduction of TG accumulation. In addition, CTE also enhanced catecholamine-induced lipolysis in adipocytes. These results suggest that CTE effectively attenuates adipogenesis by controlling cell cycle progression and downregulating adipogenic gene expression. View Full-Text
Keywords: Clitoria ternatea extract; adipogenesis; lipolysis; inhibition; adipocytes Clitoria ternatea extract; adipogenesis; lipolysis; inhibition; adipocytes
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Chayaratanasin, P.; Caobi, A.; Suparpprom, C.; Saenset, S.; Pasukamonset, P.; Suanpairintr, N.; Barbieri, M.A.; Adisakwattana, S. Clitoria ternatea Flower Petal Extract Inhibits Adipogenesis and Lipid Accumulation in 3T3-L1 Preadipocytes by Downregulating Adipogenic Gene Expression. Molecules 2019, 24, 1894.

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