Next Article in Journal
(+)-epi-Epoformin, a Phytotoxic Fungal Cyclohexenepoxide: Structure Activity Relationships
Previous Article in Journal
Nickel Sulfides Decorated SiC Foam for the Low Temperature Conversion of H2S into Elemental Sulfur
Open AccessArticle

Tetramethylpyrazine-Inducible Promoter Region from Rhodococcus jostii TMP1

Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio al. 7, LT-10257 Vilnius, Lithuania
Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Faculty of Medicine, Vilnius University, M. K. Čiurlionio 21, LT-03101 Vilnius, Lithuania
Author to whom correspondence should be addressed.
Molecules 2018, 23(7), 1530;
Received: 24 May 2018 / Revised: 15 June 2018 / Accepted: 22 June 2018 / Published: 25 June 2018
An inducible promoter region, PTTMP (tetramethylpyrazine [TTMP]), has been identified upstream of the tpdABC operon, which contains the genes required for the initial degradation of 2,3,5,6-tetramethylpyrazine in Rhodococcus jostii TMP1 bacteria. In this work, the promoter region was fused with the gene for the enhanced green fluorescent protein (EGFP) to investigate the activity of PTTMP by measuring the fluorescence of bacteria. The highest promoter activity was observed when bacteria were grown in a nutrient broth (NB) medium supplemented with 5 mM 2,3,5,6-tetramethylpyrazine for 48 h. Using a primer extension reaction, two transcriptional start sites for tpdA were identified, and the putative −35 and −10 promoter motifs were determined. The minimal promoter along with two 15 bp long direct repeats and two 7 bp inverted sequences were identified. Also, the influence of the promoter elements on the activity of PTTMP were determined using site-directed mutagenesis. Furthermore, PTTMP was shown to be induced by pyrazine derivatives containing methyl groups in the 2- and 5-positions of the heterocyclic ring, in the presence of the LuxR family transcriptional activator TpdR. View Full-Text
Keywords: Rhodococcus sp., inducible promoter; 2,3,5,6-tetramethylpyrazine; LAL subfamily; LuxR Rhodococcus sp., inducible promoter; 2,3,5,6-tetramethylpyrazine; LAL subfamily; LuxR
Show Figures

Figure 1

MDPI and ACS Style

Stanislauskienė, R.; Kutanovas, S.; Kalinienė, L.; Bratchikov, M.; Meškys, R. Tetramethylpyrazine-Inducible Promoter Region from Rhodococcus jostii TMP1. Molecules 2018, 23, 1530.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop