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Open AccessArticle

Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death

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Department of Biology, University of Louisville, Louisville, KY 40208, USA
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Department of Medicine, University of Louisville, Louisville, KY 40202, USA
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Department of Clinical Biochemistry, University of Tabuk, Tabuk 71491, Saudi Arabia
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Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, University of Arizona College of Medicine, Tucson, AZ 85724, USA
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Department of Chemistry, University of Louisville, Louisville, KY 40292, USA
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Departamento de Ciencias Celulares y Moleculares y Laboratorios de Investigacion y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 15102, Peru
*
Author to whom correspondence should be addressed.
Molecules 2018, 23(6), 1418; https://doi.org/10.3390/molecules23061418
Received: 30 May 2018 / Accepted: 8 June 2018 / Published: 12 June 2018
(This article belongs to the Section Natural Products Chemistry)
We previously reported on the isolation and biological activities of plagiochiline A (1), a 2,3-secoaromadendrane-type sesquiterpenoid from the Peruvian medicinal plant, Plagiochila disticha. This compound was found to have antiproliferative effects on a variety of solid tumor cell lines, as well as several leukemia cell lines. Other researchers have also noted the cytotoxicity of plagiochiline A (isolated from different plant species), but there are no prior reports regarding the mechanism for this bioactivity. Here, we have evaluated the effects of plagiochiline A on cell cycle progression in DU145 prostate cancer cells. A cell cycle analysis indicated that plagiochiline A caused a significant increase in the percentage of cells in the G2/M phase when compared with control cells. When cells were stained and observed by fluorescence microscopy to examine progress through the mitotic phase, we found a significant increase in the proportion of cells with features of late cytokinesis (cells connected by intercellular bridges) in the plagiochiline A-treated samples. These results suggest that plagiochiline A inhibits cell division by preventing completion of cytokinesis, particularly at the final abscission stage. We also determined that plagiochiline A reduces DU145 cell survival in clonogenic assays and that it induces substantial cell death in these cells. View Full-Text
Keywords: plagiochiline A; Plagiochila disticha; cytokinesis; abscission plagiochiline A; Plagiochila disticha; cytokinesis; abscission
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MDPI and ACS Style

Stivers, N.S.; Islam, A.; Reyes-Reyes, E.M.; Casson, L.K.; Aponte, J.C.; Vaisberg, A.J.; Hammond, G.B.; Bates, P.J. Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death. Molecules 2018, 23, 1418. https://doi.org/10.3390/molecules23061418

AMA Style

Stivers NS, Islam A, Reyes-Reyes EM, Casson LK, Aponte JC, Vaisberg AJ, Hammond GB, Bates PJ. Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death. Molecules. 2018; 23(6):1418. https://doi.org/10.3390/molecules23061418

Chicago/Turabian Style

Stivers, Nicole S.; Islam, Ashraful; Reyes-Reyes, Elsa M.; Casson, Lavona K.; Aponte, José C.; Vaisberg, Abraham J.; Hammond, Gerald B.; Bates, Paula J. 2018. "Plagiochiline A Inhibits Cytokinetic Abscission and Induces Cell Death" Molecules 23, no. 6: 1418. https://doi.org/10.3390/molecules23061418

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