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Molecules 2018, 23(6), 1362;

Tubular and Spherical SiO2 Obtained by Sol Gel Method for Lipase Immobilization and Enzymatic Activity

“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, 060021 Bucharest, Romania
Faculty of Biology, “Alexandru Ioan Cuza” University, 700505 Iasi, Romania
National Research and Development Institute for Chemistry and Petrochemistry-ICECHIM, 060021 Bucharest, Romania
Authors to whom correspondence should be addressed.
Received: 14 May 2018 / Revised: 1 June 2018 / Accepted: 1 June 2018 / Published: 5 June 2018
(This article belongs to the Special Issue Sol-Gel Chemistry. From Molecule to Functional Materials)
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A wide range of hybrid biomaterials has been designed in order to sustain bioremediation processes by associating sol-gel SiO2 matrices with various biologically active compounds (enzymes, antibodies). SiO2 is a widespread, chemically stable and non-toxic material; thus, the immobilization of enzymes on silica may lead to improving the efficiency of biocatalysts in terms of endurance and economic costs. Our present work explores the potential of different hybrid morphologies, based on hollow tubes and solid spheres of amorphous SiO2, for enzyme immobilization and the development of competitive biocatalysts. The synthesis protocol and structural characterization of spherical and tubular SiO2 obtained by the sol gel method were fully investigated in connection with the subsequent immobilization of lipase from Rhizopus orizae. The immobilization is conducted at pH 6, lower than the isoelectric point of lipase and higher than the isoelectric point of silica, which is meant to sustain the physical interactions of the enzyme with the SiO2 matrix. The morphological, textural and surface properties of spherical and tubular SiO2 were investigated by SEM, nitrogen sorption, and electrokinetic potential measurements, while the formation and characterization of hybrid organic-inorganic complexes were studied by UV-VIS, FTIR-ATR and fluorescence spectroscopy. The highest degree of enzyme immobilization (as depicted from total organic carbon) was achieved for tubular morphology and the hydrolysis of p-nitrophenyl acetate was used as an enzymatic model reaction conducted in the presence of hybrid lipase–SiO2 complex. View Full-Text
Keywords: SiO2; tubular and spherical morphology; lipase immobilization; enzymatic catalysis SiO2; tubular and spherical morphology; lipase immobilization; enzymatic catalysis

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Anastasescu, C.; Preda, S.; Rusu, A.; Culita, D.; Plavan, G.; Strungaru, S.; Calderon-Moreno, J.M.; Munteanu, C.; Gifu, C.; Enache, M.; Socoteanu, R.; Angelescu, D.; Anastasescu, M.; Gartner, M.; Balint, I.; Zaharescu, M. Tubular and Spherical SiO2 Obtained by Sol Gel Method for Lipase Immobilization and Enzymatic Activity. Molecules 2018, 23, 1362.

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