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Open AccessFeature PaperCommunication
Molecules 2018, 23(5), 1085; https://doi.org/10.3390/molecules23051085

Liposomes as Gene Delivery Vectors for Human Placental Cells

1
Chimie ParisTech, PSL Research University, University de Technologies Chimiques et Biologiques pour la Santé (UTCBS), F-75005 Paris, France
2
CNRS, UTCBS UMR 8258, F-75006 Paris, France
3
Paris Descartes University, Sorbonne-Paris-Cité, F-75006 Paris, France
4
INSERM, UTCBS U 1022, F-75006 Paris, France
5
INSERM, UMR-S1139, Faculty of pharmacy, F-75006 Paris, France
6
Laboratoire de Vectorisation Génétique, Faculty of pharmacy, Montreal University, Montreal, QC H3C 3J7, Canada
7
Foundation PremUp, F-75006 Paris, France
*
Author to whom correspondence should be addressed.
Academic Editor: Sophia G. Antimisiaris
Received: 29 March 2018 / Revised: 20 April 2018 / Accepted: 30 April 2018 / Published: 4 May 2018
(This article belongs to the Special Issue Liposomes as Drug Carriers)
Full-Text   |   PDF [1414 KB, uploaded 4 May 2018]   |  

Abstract

Nanomedicine as a therapeutic approach for pregnancy-related diseases could offer improved treatments for the mother while avoiding side effects for the fetus. In this study, we evaluated the potential of liposomes as carriers for small interfering RNAs to placental cells. Three neutral formulations carrying rhodamine-labelled siRNAs were evaluated on an in vitro model, i.e., human primary villous cytotrophoblasts. siRNA internalization rate from lipoplexes were compared to the one in the presence of the lipofectamine reagent and assessed by confocal microscopy. Results showed cellular internalization of nucleic acid with all three formulations, based on two cationic lipids, either DMAPAP or CSL-3. Moreover, incubation with DMAPAP+AA provided a rate of labelled cells as high as with lipofectamine (53 ± 15% and 44 ± 12%, respectively) while being more biocompatible. The proportion of cells which internalized siRNA were similar when using DMAPAP/DDSTU (16 ± 5%) and CSL-3 (22 ± 5%). This work highlights that liposomes could be a promising approach for gene therapy dedicated to pregnant patients. View Full-Text
Keywords: liposomes; siRNA; placenta; primary trophoblast cells liposomes; siRNA; placenta; primary trophoblast cells
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Valero, L.; Alhareth, K.; Espinoza Romero, J.; Viricel, W.; Leblond, J.; Chissey, A.; Dhotel, H.; Roques, C.; Campiol Arruda, D.; Escriou, V.; Mignet, N.; Fournier, T.; Andrieux, K. Liposomes as Gene Delivery Vectors for Human Placental Cells. Molecules 2018, 23, 1085.

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