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Molecules 2018, 23(5), 1083;

Chemical Constituents from Cimicifuga dahurica and Their Anti-Proliferative Effects on MCF-7 Breast Cancer Cells

State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
Hanoi Univerisity of Pharmacy, 13–15 Le Thanh Tong, Hanoi, Vietnam
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering (BME), Southeast University, Nanjing 210096, China
National Institute of Drug Quality Control (NIDQC), 48 Hai Ba Trung, Hoan Kiem, Hanoi, Vietnam
Author to whom correspondence should be addressed.
Received: 21 February 2018 / Revised: 15 April 2018 / Accepted: 25 April 2018 / Published: 4 May 2018
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This study was designed to search for novel anti-cancer compounds from natural plants. The 70% ethanolic extract from the rizhomes of Cimicifuga dahurica (Turcz.) Maxim. (Ranunculaceae) was found to possess significant in vitro anti-proliferative effects on MCF-7 breast cancer cells. A phytochemical investigation using assay-guided fractionation of the ethanolic extract of C. dahurica resulted in the isolation of one new phenolic amide glycoside 3, two new lignan glycosides 4 and 7, one new 9,19-cycloartane triterpenoid glycoside 6, and thirteen known constituents 1, 2, 5, and 817. The structures of 3, 4, 6, and 7 were established using contemporary NMR methods and from their HRESIMS data. The anti-proliferative effects of isolated compounds were evaluated using the BrdU-proliferation kit. Five among the 17 isolated compounds showed significant anti-proliferative effects (p ≤ 0.05), wherein compound 7 showed the most significant anti-proliferative and cell cycle arresting effect (p ≤ 0.05) which followed a dose dependent manner. Western blot protein expression analysis showed a down expression of c-Myc and cyclin D1 which further elucidated the anti-proliferation mechanism of compound 7 while apoptotic effects were found in association with Bcl-2 family protein expression variations. Conclusively this study reports the isolation and identification of 17 compounds from C. dahurica, including four novel molecules, in addition to the fact that compound 7 possesses significant anti-proliferative and apoptotic effects in vitro that may require further exploration. View Full-Text
Keywords: Cimicifuga dahurica; lignan; phenolic amide; triterpenoid glycoside; anti-proliferation Cimicifuga dahurica; lignan; phenolic amide; triterpenoid glycoside; anti-proliferation

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Huyen, C.T.T.; Luyen, B.T.T.; Khan, G.J.; Oanh, H.V.; Hung, T.M.; Li, H.-J.; Li, P. Chemical Constituents from Cimicifuga dahurica and Their Anti-Proliferative Effects on MCF-7 Breast Cancer Cells. Molecules 2018, 23, 1083.

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