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Molecules 2018, 23(4), 971; https://doi.org/10.3390/molecules23040971

Evaluation of Manganese Chloride’s Effect on Biosynthetic Properties of In Vitro Cultures of Eschscholzia californica Cham.

1
Department of Cell and Molecular Biology of Drugs, Faculty of Pharmacy, Comenius University, Kalinčiakova 8, 83232 Bratislava, Slovakia
2
Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 83232 Bratislava, Slovakia
3
Central NMR Laboratory, Faculty of Pharmacy, Comenius University, Odbojárov 10, 83232 Bratislava, Slovakia
*
Authors to whom correspondence should be addressed.
Received: 6 April 2018 / Revised: 18 April 2018 / Accepted: 19 April 2018 / Published: 21 April 2018
(This article belongs to the Section Natural Products Chemistry)
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Abstract

The basal production of secondary metabolites in medicinal plants is limited. One of the effective approaches that encourages plants to produce a remarkable amount of precious compounds is an application of elicitors. Our work was focused on the elicitation of Eschscholzia californica Cham. suspension cultures using various concentrations of MnCl2 (5; 10; 15 mg/L) with the aim of evaluating its effect on sanguinarine, chelerythrine, and macarpine production and gene expression of enzymes involved in the biosynthesis of mentioned secondary metabolites (BBE, 4′-OMT, CYP80B1) or in defense processes (LOX). Suspension cultures were exposed to elicitor for 24, 48, and 72 h. The content of alkaloids in phytomass was determined on the basis of their fluorescence properties. The relative mRNA expression of selected genes was analyzed using the ΔΔCt value method. PCR products were evaluated by melting curve analysis to confirm the specific amplification. Our results demonstrated that Eschscholzia californica Cham. cell suspension cultures evince sensitivity to the presence of MnCl2 in growth media resulting in the increased production of benzophenanthridine alkaloids and gene expression of selected enzymes. Manganese chloride seems to be a potential elicitor supporting natural biosynthetic properties in plant cell cultures and can be applied for the sustained production of valuable secondary metabolites. View Full-Text
Keywords: benzophenantridine alkaloids; berberine bridge enzyme; (S)-N-methylcoclaurine-3′-hydroxylase; 3′-hydroxy-N-methyl-(S)-coclaurine 4′-O-methyltransferase; lipoxygenase; Abiotic elicitation; Eschscholzia californica Cham.; manganese chloride benzophenantridine alkaloids; berberine bridge enzyme; (S)-N-methylcoclaurine-3′-hydroxylase; 3′-hydroxy-N-methyl-(S)-coclaurine 4′-O-methyltransferase; lipoxygenase; Abiotic elicitation; Eschscholzia californica Cham.; manganese chloride
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Balažová, A.; Urdová, J.; Bilka, F.; Holková, I.; Horváth, B.; Forman, V.; Mučaji, P. Evaluation of Manganese Chloride’s Effect on Biosynthetic Properties of In Vitro Cultures of Eschscholzia californica Cham.. Molecules 2018, 23, 971.

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