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Open AccessArticle

Identification and Comparison of Constituents of Aurantii Fructus and Aurantii Fructus Immaturus by UFLC-DAD-Triple TOF-MS/MS

1
Guangdong Engineering & Technology Research Center for Quality and Efficacy Reevaluation of Post-Market Traditional Chinese Medicine, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
2
Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China
3
Key Laboratory of Ultrafine Granular Powder of Herbal Medicine technology and Application of state Administration, Zhongshan 528437, China
*
Authors to whom correspondence should be addressed.
Molecules 2018, 23(4), 803; https://doi.org/10.3390/molecules23040803
Received: 25 January 2018 / Revised: 23 March 2018 / Accepted: 23 March 2018 / Published: 30 March 2018
(This article belongs to the Section Analytical Chemistry)
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Abstract

Although Aurantii Fructus (AF) and Aurantii Fructus Immaturus (AFI) are both the fruits of the same rutaceae plant at different stages of growth, they exert similar yet distinct clinical effects. The chemical composition is crucial for quality control as well as therapeutic application. To address this concern, it is significant to evaluate the similarities and differences of the constituents in both AF and AFI. The extract of AF and AFI were comprehensively analyzed by ultra fast liquid chromatography-photodiode array detector-triple-time of flight-tandem mass spectrometry (UFLC-DAD-Triple TOF-MS/MS). Among the 40 compounds detected, 19 metabolites were detected in both the AF and AFI; whereas 13 compounds were only detected in AF and five constituents were exclusively detected in AFI. In particular, even in AFI, three compounds were only identified in AFI (Citrus aurantium’ L. and its cultivar). Among the 18 compounds confirmed by standard database, 13 compounds were reported in AF and AFI for the first time. Furthermore, the distinction was also revealed by the content of naringin, hesperidin, neohesperidin, and synephrine. The study directly contributed to the similarities and differences of AF and AFI. Herein, similarities and the differences in chemical profiles of AF and AFI could explain the current clinical applications. View Full-Text
Keywords: Aurantii Fructus; Aurantii Fructus Immaturus; qualitative; quantitative; UFLC-DAD-Triple TOF-MS/MS Aurantii Fructus; Aurantii Fructus Immaturus; qualitative; quantitative; UFLC-DAD-Triple TOF-MS/MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Bai, Y.; Zheng, Y.; Pang, W.; Peng, W.; Wu, H.; Yao, H.; Li, P.; Deng, W.; Cheng, J.; Su, W. Identification and Comparison of Constituents of Aurantii Fructus and Aurantii Fructus Immaturus by UFLC-DAD-Triple TOF-MS/MS. Molecules 2018, 23, 803.

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