Miro1 Enhances Mitochondria Transfer from Multipotent Mesenchymal Stem Cells (MMSC) to Neural Cells and Improves the Efficacy of Cell Recovery
Abstract
:1. Introduction
2. Results
2.1. Transfer of Mitochondria from MMSC to Neural Cells
2.2. The Transfer of Mitochondria Can Occur through Tunneling Nanotubes
2.3. The Transport of Mitochondria Restores Cell Proliferation and Respiration
2.4. Effect of Miro1 Overexpression on Mitochondrial Transfer
3. Discussion
4. Materials and Methods
4.1. Preparation of Astroglial Cells
4.2. MMSC
4.3. Co-Cultivation of Rat Astrocytes and MMSC
4.4. Oxygen-Glucose Deprivation Protocol
4.5. PC12-ρ0 Cell Line
4.6. Confocal Microscopy
4.7. Proliferation and Bioenergetics Assays
4.8. Viral Constructs
4.9. Middle Cerebral Artery Occlusion Model of Focal Ischemia
4.10. Statistics
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Babenko, V.A.; Silachev, D.N.; Popkov, V.A.; Zorova, L.D.; Pevzner, I.B.; Plotnikov, E.Y.; Sukhikh, G.T.; Zorov, D.B. Miro1 Enhances Mitochondria Transfer from Multipotent Mesenchymal Stem Cells (MMSC) to Neural Cells and Improves the Efficacy of Cell Recovery. Molecules 2018, 23, 687. https://doi.org/10.3390/molecules23030687
Babenko VA, Silachev DN, Popkov VA, Zorova LD, Pevzner IB, Plotnikov EY, Sukhikh GT, Zorov DB. Miro1 Enhances Mitochondria Transfer from Multipotent Mesenchymal Stem Cells (MMSC) to Neural Cells and Improves the Efficacy of Cell Recovery. Molecules. 2018; 23(3):687. https://doi.org/10.3390/molecules23030687
Chicago/Turabian StyleBabenko, Valentina A., Denis N. Silachev, Vasily A. Popkov, Ljubava D. Zorova, Irina B. Pevzner, Egor Y. Plotnikov, Gennady T. Sukhikh, and Dmitry B. Zorov. 2018. "Miro1 Enhances Mitochondria Transfer from Multipotent Mesenchymal Stem Cells (MMSC) to Neural Cells and Improves the Efficacy of Cell Recovery" Molecules 23, no. 3: 687. https://doi.org/10.3390/molecules23030687